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作物学报 ›› 2013, Vol. 39 ›› Issue (06): 979-991.doi: 10.3724/SP.J.1006.2013.00979

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

基于叶绿体DNA序列的Ceratotropis亚属遗传进化研究

刘岩,程须珍*,王丽侠,王素华,白鹏   

  1. 中国农业科学院作物科学研究所,北京100081
  • 收稿日期:2012-11-16 修回日期:2013-01-15 出版日期:2013-06-12 网络出版日期:2013-03-20
  • 通讯作者: 程须珍, E-mail: chengxz@caas.net.cn, Tel: 010-62180535
  • 基金资助:

    本研究由北京市自然科学基金项目(6103025), 国家现代农业产业技术体系建设专项(CARS-09)和国家公益性行业(农业)科研专项(nyhyzx07-017)资助。

Genetic Evolution for Vigna Subgenus Ceratotropis Based on Chloroplast DNA Sequences

LIU Yan,CHENG Xu-Zhen*,WANG Li-Xia,WANG Su-Hua,BAI Peng   

  1. Genetic Evolution for Vigna Subgenus Ceratotropis Based on Chloroplast DNA Sequences
  • Received:2012-11-16 Revised:2013-01-15 Published:2013-06-12 Published online:2013-03-20
  • Contact: 程须珍, E-mail: chengxz@caas.net.cn, Tel: 010-62180535

摘要:

作为Ceratotropis亚属起源中心之一,中国有丰富的Ceratotropis亚属种质资源,但种间遗传进化研究相对落后。为探索该亚属各种间的亲缘关系及遗传演化规律,对Ceratotropis亚属18个种及亚种共110份材料的叶绿体基因组2个内含子(rpl16rps16)3个基因(psbA-trnHrpoB-trnCtrnL-trnF)的间隔区序列进行了研究。结果表明,各序列的信息位点百分率在3.63%~24.28%之间,其中rps16最高,rpoB-trnC最低。序列拼接的Kimura进化距离分析发现,各种间进化距离介于0~0.057之间,V. minima复合体(V. minimaV. riukinensisV. nakashime)V. subramaniana的进化距离最大。基于单个序列,利用MEGA5.1PAUP4.0软件构建的邻接树和最大简约树的结果均将Ceratotropis亚属18个种及亚种划分为2大支,其中第I类包含9个种和亚种,为3个组(AngularesCeratotropisAconitifoliae)的混合体,II类也包含9个种和亚种,全部属于Angulares组。系统发育树的分化同质性检验表明其拓扑一致性较高(P=0.87)psbA-trnH5个序列的分子系统进化研究对阐明Ceratotropis亚属种间亲缘关系和系统演化具有重要的理论和现实意义。

关键词: Ceratotropis亚属, 叶绿体DNA, 序列差异, 遗传进化

Abstract:

China has abundant germplasm resources of subgenus Ceratotropis, but the related study of species within this subgenus is much lagged. In order to investigate genetic relationship and evolution process among different species, 110 entries of Ceratotropis were analyzed by using intron rpl16, rps16 and intergenic regions psbA-trnH, rpoB-trnC, and trnL-trnF of chloroplast genome. The results revealed that parsim-info site percentage was from 3.63% to 24.28%, with the highest for rps16 and the lowest for rpoB-trnC. A higher level conservation of sequence was observed within species than inter-species. Kimura-2-parameter distance was between 0 and 0.057 calculated based on five chloroplast assembling sequences. V. minima complex (including V. minima, V. riukinensis and V. nakashime) and V. subramaniana had the longest distance. Phylogenetic trees constructed by neighbor-joining (NJ) and maximum parsimony (MP) methods in MEGA 5.1 and PAUP 4.0 revealed that eighteen species and subspecies were divided into two branches. The group I contained nine species and subspecies grouped into three sections complex, including Angulares, Ceratotropis and Aconitifoliae. The group II contained nine species and subspecies which completely belonged to section Angulares. Partition homogeneity test of phylogenetic trees of the five sequences showed that their topology was highly consistent (P=0.87). The present study provides the important information for clarifying genetic relationship Ceratotropis. among different species within

Key words: Subgenus Ceratotropis, Chloroplas DNA, Sequence difference, Genetic evolution

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