作物学报 ›› 2013, Vol. 39 ›› Issue (06): 979-991.doi: 10.3724/SP.J.1006.2013.00979
刘岩,程须珍*,王丽侠,王素华,白鹏
LIU Yan,CHENG Xu-Zhen*,WANG Li-Xia,WANG Su-Hua,BAI Peng
摘要:
作为Ceratotropis亚属起源中心之一,中国有丰富的Ceratotropis亚属种质资源,但种间遗传进化研究相对落后。为探索该亚属各种间的亲缘关系及遗传演化规律,对Ceratotropis亚属18个种及亚种共110份材料的叶绿体基因组2个内含子(rpl16、rps16)和3个基因(psbA-trnH、rpoB-trnC和trnL-trnF)的间隔区序列进行了研究。结果表明,各序列的信息位点百分率在3.63%~24.28%之间,其中rps16最高,rpoB-trnC最低。序列拼接的Kimura进化距离分析发现,各种间进化距离介于0~0.057之间,V. minima复合体(V. minima、V. riukinensis和V. nakashime)和V. subramaniana的进化距离最大。基于单个序列,利用MEGA5.1和PAUP4.0软件构建的邻接树和最大简约树的结果均将Ceratotropis亚属18个种及亚种划分为2大支,其中第I类包含9个种和亚种,为3个组(Angulares、Ceratotropis和Aconitifoliae)的混合体,第II类也包含9个种和亚种,全部属于Angulares组。系统发育树的分化同质性检验表明其拓扑一致性较高(P=0.87)。psbA-trnH等5个序列的分子系统进化研究对阐明Ceratotropis亚属种间亲缘关系和系统演化具有重要的理论和现实意义。
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