作物学报 ›› 2014, Vol. 40 ›› Issue (06): 1132-1139.doi: 10.3724/SP.J.1006.2014.01132
• 研究简报 • 上一篇
魏春燕1,邢永秀1,2,*,莫遥1,林丽2,杨丽涛1,2,3,胡春锦3,李杨瑞1,2,3,*
WEI Chun-Yan1,XING Yong-Xiu1,2,*,MO Yao1,LIN Li2,YANG Li-Tao1,2,3,HU Chun-Jin3,LI Yang-Rui1,2,3,*
摘要:
为了探索甘蔗固氮菌DX120E菌株侵入根的方式以及菌株在甘蔗植株内的定殖规律, 确定人工接种固氮菌适宜的侵染浓度和定殖特点, 采用绿色荧光蛋白(GFP)标记的固氮菌DX120E接种甘蔗组培苗, 以荧光显微镜观察其侵染和定殖情况, 以抗生素抗性标记法和平板分离计数法测定DX120E菌株的种群动态。结果表明, 菌株DX120E在2个甘蔗品种的根、叶鞘和叶内均能定殖, 定殖菌量依次均为根>叶鞘>叶; 不同接种浓度下, 最大定殖数量无显著差异, 102 CFU mL–1的接种量足够侵入甘蔗并积累定殖。DX120E从甘蔗根表面的裂隙、主根和侧根发生处及根的断裂处均可侵入, 主要在根表面细胞间隙和细胞内大量定殖, 同时也可迁移到叶片的叶肉细胞和维管束细胞中定殖。
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