作物学报 ›› 2020, Vol. 46 ›› Issue (7): 1128-1133.doi: 10.3724/SP.J.1006.2020.93056
• 研究简报 • 上一篇
摘要:
为填补我国在伏马毒素基体标准物质的空白, 本试验研制了以玉米粉为基体的伏马毒素FB1标准物质, 玉米样品经过筛、加标、冷冻干燥、磨粉、混匀、密封分装后, 用超高效液相色谱-串联质谱(UPLC-MS/MS)法检验样品的均匀性、稳定性, 并联合多家实验室对玉米粉中伏马毒素FB1含量定值, 同时分析样品的不确定度。样品均匀性经方差分析法表明F值为1.42, 小于临界值F0.05, 且伏马毒素FB1含量在规定时间6个月内无明显变化。结果表明, 均匀性与稳定性均符合标准物质的要求。样品定值为1475.56 μg kg-1, 不确定度为169.98 μg kg-1。该标准物质可替代进口标准物质, 用于伏马毒素检测过程中的仪器校准、实验室质量控制和操作人员的水平考核等。
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