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作物学报 ›› 2020, Vol. 46 ›› Issue (8): 1135-1145.doi: 10.3724/SP.J.1006.2020.03003

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

玉米种质资源大规模多年多点多病害的自然发病抗性鉴定

段灿星1,董怀玉2,李晓3,李红4,李春辉1,孙素丽1,朱振东1,王晓鸣1,*()   

  1. 1中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程, 北京100081
    2辽宁省农业科学院植物保护研究所, 辽宁沈阳110161
    3四川省农业科学院植物保护研究所, 四川成都 610066
    4吉林省农业科学院植物保护研究所, 吉林公主岭 136100
  • 收稿日期:2020-01-17 接受日期:2020-03-24 出版日期:2020-08-12 网络出版日期:2020-04-07
  • 通讯作者: 王晓鸣
  • 作者简介:E-mail: duancanxing@caas.cn
  • 基金资助:
    国家重点研发计划项目(2016YFD0100103);作物种质资源保护专项(2019NWB036-12)

A large-scale screening of maize germplasm for resistance to multiple diseases in multi-plot demonstration for several years under natural condition

DUAN Can-Xing1,DONG Huai-Yu2,LI Xiao3,LI Hong4,LI Chun-Hui1,SUN Su-Li1,ZHU Zhen-Dong1,WANG Xiao-Ming1,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China
    2Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, Liaoning, China
    3Institute of Plant Protection, Sichuan Academy of Agricultural Sciences, Chengdu 610066, Sichuan, China
    4Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin, China
  • Received:2020-01-17 Accepted:2020-03-24 Published:2020-08-12 Published online:2020-04-07
  • Contact: Xiao-Ming WANG
  • Supported by:
    National Key Research and Development Program of China(2016YFD0100103);Special Fund for Protection of Crop Germplasm Resources(2019NWB036-12)

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摘要：

病害是影响玉米生产的重要因素。利用品种的抗性是控制玉米病害的经济、安全和有效措施。2016—2019年间, 在黄淮海和东华北地区, 首次对2000份来源广泛且遗传背景丰富的玉米种质资源进行了多年多点多病害的田间自然发生条件下抗病性鉴定, 部分材料在西北地区也进行了田间鉴定, 重点调查了小斑病、茎腐病、瘤黑粉病、弯孢叶斑病、南方锈病、粗缩病、大斑病、灰斑病和丝黑穗病的抗病性。综合4年共10个不同环境的自然发病下抗性鉴定数据表明, 自然发病鉴定的结果受环境因素影响较大, 表现为年度间和地域间的差异。在所有鉴定的病害中, 小斑病在多个年份和多个鉴定点的发病均比较充分, 11份种质对该病害表现出稳定抗性; 茎腐病、大斑病和灰斑病, 在不同年份的部分鉴定点发病较为充分, 对这3种病害表现抗病的种质分别为440、356和423份, 综合抗性鉴定结果具有较大的参考价值; 弯孢叶斑病、瘤黑粉病和粗缩病仅在1个鉴定点发病较为充分, 南方锈病和丝黑穗病在所有鉴定点均发生较轻, 鉴定结果有待进一步验证。本研究筛选出一批在不同环境条件下对多种病害均具有稳定抗性的材料, 其中JN15、953、沈977、68122、K21、SC24-1、17MC7211、17MC7223、郑591、161191等种质的综合抗性突出, 对种质的利用及后续人工接种精准鉴定的种质选择具重要参考价值。

关键词: 玉米种质, 病害, 多年多点, 自然发病鉴定

Abstract:

Disease is a major factor affecting maize production. The utilization of resistant cultivars is the most economical, safe, and effective method for controlling maize diseases. This research was finally conducted using 2000 maize germplasm accessions with extensive sources and rich genetic background to identify and evaluate the resistance to multiple diseases in multi-plot demonstration for several years under natural condition during 2016-2019. Nine diseases, including southern corn leaf blight, stalk rot, common smut, Curvularia leaf spot, southern rust, maize rough dwarf, northern corn leaf blight, gray leaf spot and head smut were investigated and evaluated in non-inoculated fields. The experimental data at 10 different sites from 2016 to 2019 showed that resistance identification under natural conditions was sensitive to environmental factors, with distinct differences among years and regions. The incidence of southern corn leaf blight was severe at multiple sites over these years and 11 maize accessions with stable resistance were screened out. Stalk rot, northern corn leaf blight, and gray leaf spot were relatively serious at several sites in four years, and 440, 356, and 423 resistant germplasm were screened out from 2000 accessions, respectively. These integrated data had considerable reference values. Curvularia leaf spot, common smut, and maize rough dwarf occurred heavily at only one plot while southern rust and head smut were not serious at all experimental sites, which should be studied further more. All in all, some maize germplasm resources with stable resistance to diverse diseases under different environments were screened out, such as JN15, 953, Shen 977, 68122, K21, SC24-1, 17MC7211, 17MC7223, Zheng 591, 161191, and so on. These results provide a significant reference for utilization and selection of germplasm for further precise inoculated identification.

Key words: maize germplasm, disease, multi-plot demonstration for several years, non-inoculated resistance identification

表1

2016-2019年间11份种质在各鉴定点的小斑病病情级别与综合抗性水平"

种质名称
Name		 2016 2017 2018 2019 最高
病级Scale		 综合抗性Resistance
北京
BJ		 河南
HN		 山东
SD		 北京
BJ		 河南HN 山东
SD		 北京
BJ		 河南HN 山东
SD		 北京
BJ		 河南HN 山东
SD
H599a 5 5 5 5 3 5 3 3 5 3 3 3 5 MR
HRB16198 5 3 3 3 3 5 3 5 3 3 3 3 5 MR
MC7470 5 3 3 5 5 3 3 3 3 5 3 1 5 MR
MC7480 5 5 5 5 5 5 5 5 5 3 3 3 5 MR
MC7487 3 3 5 5 3 5 3 3 3 1 3 3 5 MR
MC7527 3 3 5 5 5 5 3 5 3 1 3 3 5 MR
MC7542 5 3 3 5 3 5 3 5 3 1 3 3 5 MR
MC7549 3 3 5 5 5 5 5 3 5 3 3 3 5 MR
W-25 3 3 5 5 3 5 3 3 3 1 3 3 5 MR
X324 5 3 3 5 3 5 3 5 3 1 3 3 5 MR
绵983 Mian 983 5 3 3 3 5 5 5 3 3 3 3 3 5 MR

表2

2016-2019年田间自然发病鉴定中对茎腐病表现高抗的部分种质"

名称
Name		 发病率
Incidence (%)		 名称
Name		 发病率
Incidence (%)		 名称
Name		 发病率
Incidence (%)		 名称
Name		 发病率
Incidence (%)
16SD001 4.2 HRB16210 0 Y378 4.5 辽20382 Liao 20382 4.5
16SD007 4.8 J005 4.8 丹1133 Dan 1133 0 辽2309 Liao 2309 0
16SD069 0 M44928 4.3 丹599 Dan 599 4.2 辽5090 Liao 5090 4.5
16SD082 4.3 M45638 4.2 吉资1046 Jizi 1046 0 辽785 Liao 785 0
16SD119 4.5 M5168 4.8 吉资1137 Jizi 1137 4.0 辽8821 Liao 8821 4.2
16SD122 0 MC7452 4.5 吉资1138 Jizi 1138 4.5 陇0988 Long 0988 4.8
16SD153 3.8 MC7470 4.5 冀SX32T29 Ji SX32T29 3.8 陇1103 Long 1103 0
16SD176 0 MC7473 0 冀SX32T31 Ji SX32T31 4.5 陇1307 Long 1307 0
214CTS 0 MC7494 0 冀资13W142 Jizi 13W142 0 陇1401 Long 1401 3.6
5N76 4.2 MC7504 4.2 冀资14W108 Jizi 14W108 4.2 陇M0304 Long M0304 0
C89 0 MC7549 4.2 冀资14W290 Jizi 14W290 3.6 沈3336 Shen 3336 3.8
D26 4.8 X102 0 冀资H635 Jizi H635 0 H06-71 4.2
DN6217 0 X324 0 冀资S187 Jizi S187 4.5 铁0255-1 Tie 0255-1 0
DXA001 4.2 Y1630 4.2 冀资S735 Jizi S735 4.5 铁20135 Tie 20135 4.0
FZ30 0 Y1632 0 R8-96 4.5 铁7922 Tie 7922 0
G285 0 Y1680 4.5 X8531 0 PH2VK 4.3
H050 0 Y1685 4.5 CH7-2-1 3.6 PH6JM 3.4
HRB16074 0 Y1687 4.3 Ds-12a 3.8 PHPMO 0
HRB16101 4.5 Y1711 4.8 X901 3.6 W314 3.8
HRB16109 0 Y1720 0. 浚248 Xun 248 3.8 X48B 4.8
HRB16134 3.8 Y1754 4.2 HRB16209 3.8 运系98-3 Yunxi 98-3 4.2
HRB16142 0 Y1755 4.2 辽0558 Liao 0558 0 辽106 Liao 106 0

表3

2016-2019年田间自然发病鉴定中对瘤黑粉病表现高抗的部分种质"

名称
Name		 发病率
Incidence (%)		 名称
Name		 发病率
Incidence (%)		 名称
Name		 发病率
Incidence (%)		 名称
Name		 发病率
Incidence (%)
16SD033 0 JLXH-3 0 P138 0 冀资B2042 Jizi B2042 0
16SD069 0 JMO17D 0 PH298 0 冀资R16 Jizi R16 0
16SD176 0 K12 0 PHP60 0 冀资S187 Jizi S187 0
16SD179 0 KP105 0 SF8 0 辽0567 Liao 0567 0
2015S1093 0 L98 0 T0294 0 辽106 Liao 106 0
81-4-2 0 ly5502 0 T24139 0 辽20382 Liao 20382 0
C89 0 M09N142 0 T33448 0 辽2572 Liao 2572 0
DN1722 0 M10NJ49 0 T5195 0 辽63114 Liao 63114 0
DXA105 0 M15131 0 T7309 0 陇0986 Long 0986 0
DXA119 0 M35276 0 T98131 0 陇1107 Long 1107 0
DXA168 0 M44928 0 TE350 0 陇1447 Long 1447 0
DXB030 0 M45482 0 W240 0 陇M0304 Long M0304 0
DXB060 0 M45845 0 W9813 0 陇M0801 Long M0801 0
DXB171 0 MC3361 0 X47B 0 陇M0818 Long M0818 0
DXB198 0 MC5836 0 Y1710 0 明2325 Ming 2325 0
F665 0 MC5843 0 Y1740 0 沈135 Shen 135 0
FZ30 0 MC6172 0 Y1741 0 沈3336 Shen 3336 0
HRB16003 0 MC6180 0 Y1747 0 绥系608 Suixi 608 0
HRB16006 0 MC7457 0 YCM 0 铁0519 Tie 0519 0
HRB16187 0 MC7546 0 合系15-17 Hexi 15-17 0 铁20135 Tie 20135 0
HRB16201 0 MC9292 0 吉资1040 Jizi 1040 0 郑32P75 Zheng 32P75 0
JLXH-11 0 MC9295 0 吉资1140 Jizi 1140 0 中451 Zhong 451 0
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