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作物学报 ›› 2021, Vol. 47 ›› Issue (8): 1624-1630.doi: 10.3724/SP.J.1006.2021.01069

• 研究简报 • 上一篇    

大麦籽粒发育进程中7种黄酮类化合物的积累特性分析

贺军与(), 钟伟, 陈云琼, 王卫斌, 熊静蕾, 蒋亚丽, 施辉蒙, 陈升位*()   

  1. 云南农业大学农学与生物技术学院, 云南昆明 650201
  • 收稿日期:2020-08-28 接受日期:2021-01-14 出版日期:2021-08-12 网络出版日期:2021-02-19
  • 通讯作者: 陈升位
  • 作者简介:E-mail: etfiee_coisini@126.com
  • 基金资助:
    国家自然科学基金项目(32060457)

Analysis on the accumulation characteristics of seven flavonoids at grain development stage in barley

HE Jun-Yu(), ZHONG Wei, CHEN Yun-Qiong, WANG Wei-Bin, XIONG Jing-Lei, JIANG Ya-Li, SHI Hui-Meng, CHEN Sheng-Wei*()   

  1. Collage of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, Yunnan, China
  • Received:2020-08-28 Accepted:2021-01-14 Published:2021-08-12 Published online:2021-02-19
  • Contact: CHEN Sheng-Wei
  • Supported by:
    National Natural Science Foundation of China(32060457)

摘要:

解析大麦籽粒中黄酮类化合物的积累特性有利于其含量检测、积累机制和生理功能研究。本文采用高效液相色谱测定了8个大麦材料籽粒、7个发育时期15种黄酮类化合物的含量。在7个时期, 15种化合物总含量呈上升—下降的变化趋势, 在花后10 d最高(335.71± 41.63) μg g-1, 成熟籽粒最低(165.52±38.63) μg g-1。花后10 d曲克芦丁、芹菜素、短柄花素和白杨素的含量最高, 分别为(155.75±30.76) μg g-1、(8.41±1.54) μg g-1、(10.71±1.53) μg g-1和(4.41±0.60) μg g-1; 花后5 d柚皮素查尔酮、花后15 d二氢槲皮素和花后25 d木犀草素的含量最高, 分别为(42.41±15.39) μg g-1、(17.92±6.60) μg g-1和(5.19±1.37) μg g-1。其中, 柚皮素查尔酮的含量变化趋势为下降—平台—下降; 曲克芦丁和白杨素的含量变化趋势为上升—下将—平台—下降; 二氢槲皮素、木犀草素、芹菜素和芒柄花素的含量变化趋势为上升—下降。在含量检测等研究中, 大麦籽粒曲克芦丁、芹菜素、短柄花素和白杨素的适宜取样时间均为花后10 d, 柚皮素查尔酮、二氢槲皮素和木犀草素的适宜取样时间分别为花后5、15和25 d。上述结果为大麦籽粒黄酮类化合物含量测定等研究提供了理论支持。

关键词: 大麦籽粒, 发育时期, 黄酮类化合物, 积累特性

Abstract:

The analysis on the accumulation characteristics of flavonoids in barley grain is helpful for the studies, such as the content detection, accumulation mechanism and physiological function of these compounds. In this study, the contents of 15 flavonoids of eight barley material grains during seven development stages were determined by HPLC. The total contents of 15 compounds were exhibited the trend of up-down variation, reaching the highest value (335.71±41.63) μg g-1 on the 10th day after anthesis, and the lowest (165.52±38.63) μg g-1 in mature grain. The highest contents of troxerutin, apigenin, formononetin, and chrysin were (155.75±30.76) μg g-1, (8.41±1.54) μg g-1, (10.71±1.53) μg g-1, and (4.41±0.60) μg g-1 on the 10th day after anthesis, respectively. The highest contents of naringenin chalcone, dihydroquercetin, and luteolin were (42.41±15.39) μg g-1, (17.92±6.60) μg g-1, and (5.19±1.37) μg g-1 on the 5th, 15th, and 25th day after anthesis, respectively. The content of naringenin chalcone was changed with down-platform-down trend during development of barley grain, but the contents of troxerutin and chrysin were changed with up-down-platform-down trend, and the contents of dihydroquercetin, luteolin, apigenin and formononetin were changed with up-down trend. In terms of flavonoids content detection, the appropriate sampling time of for troxerutin, apigenin, formononetin and chrysin in barley grains was 10 days after post-anthesis stage, and the appropriate time of naringenin chalcone, dihydroquercetin, and luteolin was 5, 15, and 25 days after post-anthesis stage. These results can provide the theoretical support for the content detection of flavonoids in barley grains.

Key words: barley grain, development stage, flavonoids, accumulation characteristics

表1

试验材料及其基本特性"

材料编号
Code of materials
材料名称
Name
籽粒颜色
Coat color of grains
材料类型
Material type
Yel1 Bowman 黄色籽粒Yellow grain coat 育成品种Improved variety
Yel2 Morex 黄色籽粒Yellow grain coat 育成品种Improved variety
Yel3 光头大麦Guangtoudamai 黄色籽粒Yellow grain coat 地方品种Landrace
Pur1 淳安六棱胭脂大麦Chunanliulengyanzhidamai 紫色籽粒Purple grain coat 地方品种Landrace
Pur2 紫青稞Purple highland barley 紫色籽粒Purple grain coat 育成品种Improved variety
Pur3 定海红绿黄Dinghaihonglyuhuang 紫色籽粒Purple grain coat 地方品种Landrace
Bla 云南高世代Yunnan gaoshidai 黑色籽粒Black grain coat 育成品系Developed line
Blu 北青7号Beiqing 7 蓝色籽粒Blue grain coat 育成品系Improved variety

图1

大麦籽粒7个发育时期15种化合物总含量曲线 T1~T7: 花后5、10、15、20、25、30 d和成熟籽粒。"

表2

在不同发育时期特定化合物的平均含量(μg g-1)及其差异"

化合物
Compound
T1 T2 T3 T4 T5 T6 T7
曲克芦丁
Troxerutin
91.75±23.78
bc ABC
155.75±30.76
a A
134.88±24.36
ab AB
65.23±27.44
cd B
56.07±12.95
cd BC
53.42±16.27
cd BC
21.64±6.37
d C
二氢槲皮素
Dihydroquercetin
6.52±2.37
cd BC
21.80±5.19
a A
17.92±6.60
ab AB
16.48±3.97
abc ABC
13.37±3.17
abcd ABC
9.55±2.82
bcd ABC
2.63±0.90
d C
木犀草素
Luteolin
0.60±0.36
b B
1.75±0.89
b AB
1.32±0.68
b AB
5.02±1.97
a A
5.19±1.37
a A
3.45±1.10
ab AB
3.49±0.87
ab AB
柚皮素查尔酮
Naringenin chalcone
42.41±15.39
a A
11.31±6.69
b AB
13.63±12.95
b AB
10.23±7.89
b AB
6.60±6.60
b B
0.00±0.00
b B
4.17±2.36
B B
芹菜素
Apigenin
7.31±1.81
ab A
8.41±1.54
a A
5.51±0.80
abc AB
5.81±0.94
ab AB
4.23±1.00
bcd AB
2.47±0.61
cd B
2.24±1.03
c B
芒柄花素
Formononetin
7.83±3.03
ab AB
10.71±1.53
a A
10.71±1.25
a A
6.67±0.59
ab ABC
5.84±0.43
b ABC
4.21±0.48
bc BC
1.44±0.71
c C
白杨素
Chrysin
1.48±0.53
cd BC
4.41±0.60
a A
2.90±0.30
b B
1.58±0.18
c BC
1.98±0.30
bc B
1.76±0.23
c BC
0.42±0.32
d C

图2

籽粒发育进程中7种化合物含量测定曲线 A~G: 柚皮素查尔酮、曲克芦丁、二氢槲皮素、木犀草素、芹菜素、短柄花素和白杨素; T1~T7: 花后5、10、15、20、25、30 d和成熟籽粒。"

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