作物学报 ›› 2024, Vol. 50 ›› Issue (9): 2179-2186.doi: 10.3724/SP.J.1006.2024.44030
孙现军1(), 胡正1, 姜雪敏2, 王世佳2, 陈向前2, 张惠媛1, 张辉1,*(
), 姜奇彦2,*(
)
SUN Xian-Jun1(), HU Zheng1, JIANG Xue-Min2, WANG Shi-Jia2, CHEN Xiang-Qian2, ZHANG Hui-Yuan1, ZHANG Hui1,*(
), JIANG Qi-Yan2,*(
)
摘要:
土壤盐渍化严重影响大豆品质与产量, 筛选耐盐大豆资源对开展盐碱地综合利用意义重大。为建立大豆苗期耐盐鉴定评价体系, 设置淡水和NaCl含量为0.9%~1.8%的10个等差梯度, 以蛭石为培养基质, 大豆2片真叶始现时开始盐处理。结果表明, 1.2%盐处理16 d时, 不同大豆种质资源耐盐等级四分位差值最大, 是大豆苗期耐盐鉴定评价的最适条件。利用大豆苗期耐盐鉴定评价体系对来自国内外的504份大豆种质资源进行苗期耐盐性鉴定评价, 耐盐等级为1级、2级、3级、4级、5级的大豆资源依次为46份、146份、157份、79份、76份。利用GmSALT3基因的分子标记对1级耐盐资源进行检测, 其中40份(86.96%)大豆材料扩增结果与GmSALT3基因的分子标记结果相符合。为分析大豆苗期鉴定过程中盐胁迫浓度的变化趋势, 确立了土壤含盐量(Y, %)与电导率(X, mS cm-1)的回归方程: Y=0.278X-0.0618, 预测精准度在95%以上。测定统计了从盐处理开始至调查结束时的培养基质含盐量变化趋势, 培养基质含盐量基本维持在13 mS cm-1左右。本研究为大豆苗期规模化耐盐性鉴定和培育耐盐新种质提供了技术体系和基础材料。
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