作物学报 ›› 1989, Vol. 15 ›› Issue (02): 141-145.
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卫俊智; 朱凤绥
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Wei Junzhi Zhu Fengsui
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摘要： AMD 法显带技术对一粒小麦的染色体进行染色体高分辨显带,经过放线菌素 D 预处理、Ohnuki′s 溶液低渗、固定液中捣碎涂片法制片、Wright-Giemsa 染色等显带程序,可以在一粒小麦的所有染色体上成功地显示丰富的高分辨带纹,并对其进行了分析与配对,平均每条染色体上有20多条深带,整个染色体组共显143条带纹。这将对一粒小麦染
关键词: 一粒小麦, 高分辨显带, AMD法,
Abstract: A new banding technique,named AMD(actinomycin D)method,has been carried out in chromosomes of Triticum monococcum.The technique mainly includes:pretreatment with AMD,hypotonic treatment in Ohnuki's solution,chromosomalpreparation by smash and smear,and staining with Wright-Giemsa.This methodcan prod
Key words: Triticum monococcum, High resolution banding, Actinomycin D
卫俊智; 朱凤绥. 一粒小麦染色体高分辨显带[J]. 作物学报, 1989, 15(02): 141-145.
Wei Junzhi Zhu Fengsui. High Resolution Banding in Chromosomes of Triticum monococcum[J]. Acta Agron Sin, 1989, 15(02): 141-145.
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