玉米获白×莱1029的悬浮细胞系的建立及其再生植株的研究

作物学报 ›› 1994, Vol. 20 ›› Issue (02): 168-175.

玉米获白×莱1029的悬浮细胞系的建立及其再生植株的研究

孙世孟;赛吉庆;谢友菊

北京农业大学生物学院，北京，100094

收稿日期:1992-09-10 修回日期:1993-03-26 出版日期:1994-03-12 网络出版日期:1994-03-12
通讯作者: 孙世孟

Study of Establishment of H × L Genotype Suspension Cell Lines Capable of Plant Regeneration in Maize

Sun Shi-meng; Sai Ji-qing; Xie You-ju

College of Biology, Beijing Agricultural University, Beijing,100094

Received:1992-09-10 Revised:1993-03-26 Published:1994-03-12 Published online:1994-03-12
Contact: Sun Shi-meng

摘要/Abstract

摘要： 利用玉米获白×莱1029的未成熟胚诱导的愈伤组织，系统地研究了愈伤组织的改良、悬浮细胞系的建立及其分化。结果表明，获白×莱1029的愈伤组织在高硝态氮的培养基上，经过调节2，4-D的浓度、渗透压和NaCl的浓度，选择得到了黄色、小颗粒状结构、不分泌粘液、快速生长和易于悬浮的松脆愈伤组织；在悬浮培养初期，经过高浓度2

关键词: 玉米悬浮细胞，组织培养，植株再生

Abstract: The whole establishment process of suspension cell lines, including callus induction, callus texture modification, establishment and differentiation of suspension celllines, was systematically studied with immature embryos from H×L genotype in maize. A kind of golden, non-mucilaginous, grainy textu

Key words: Maize suspension cells, Maize cell culture, Plant regeneratoin.

孙世孟;赛吉庆;谢友菊. 玉米获白×莱1029的悬浮细胞系的建立及其再生植株的研究[J]. 作物学报, 1994, 20(02): 168-175.

Sun Shi-meng; Sai Ji-qing; Xie You-ju. Study of Establishment of H × L Genotype Suspension Cell Lines Capable of Plant Regeneration in Maize[J]. Acta Agron Sin, 1994, 20(02): 168-175.

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