籼稻体细胞胚胎发生特异性蛋白质研究

作物学报 ›› 1994, Vol. 20 ›› Issue (04): 395-400.

籼稻体细胞胚胎发生特异性蛋白质研究

方继朝;张金渝;薛庆中;吴光南

江苏省农业科学院农业生物遗传生理研究所，农业部农业生物学实验室，江苏南京，210014

收稿日期:1992-09-04 修回日期:1993-03-28 出版日期:1994-07-12 网络出版日期:1994-07-12
通讯作者: 方继朝

Studies on Specific Proteins of Somatic Embryogenesis in Oryz Sativa L. subsp. Indica

Fang Ji-chao; Zhang Jin-ru; Xue-Qin-zhong; Wu Guang-nan

Laboratory of Agrobiology, Ministry of Agriculture. Institute of Agrobiological Genetics & Physiology, Jiangsu Academy of Agricultural Sciences. Nanjing 210014

Received:1992-09-04 Revised:1993-03-28 Published:1994-07-12 Published online:1994-07-12
Contact: Fang Ji-chao

摘要/Abstract

摘要： 以籼稻Oryza sativa L.subsp. indica品种“广陆矮4号”幼穗和种子为外植体，在含有2，4-D2.0mg/L的MS培养基上，分别形成胚性和非胚性愈伤组织，建立体细胞胚胎发生实验系统。应用Native/SDS双向凝胶电泳分析，结果表明，非胚性愈伤组织分化培养前后均存在一种分子量为45kD的非胚性蛋白质N1，另一种非胚性蛋白质N2（54kD）仅

关键词: 籼稻，体细胞胚胎发生，双向凝胶电泳，非胚性蛋白质，胚胎蛋白质

Abstract: An experimental system of somatic embryogenesis in Oryza sativa L. subsp. indica was established, which involved embryogenic calli induced from rudimentary panicles and nonembryogenic ones from mature seeds of Guangluai No.4(cv.), respectively, on a MS basal medium with 2.0 mg/L 2,4-D. Profiles of s

Key words: Oryza sativa L. subsp. indica, Somatic embryogenesis, Native/SDS 2D PAGE, Non-embryonic protein, Embryo-specific proteins

方继朝;张金渝;薛庆中;吴光南. 籼稻体细胞胚胎发生特异性蛋白质研究[J]. 作物学报, 1994, 20(04): 395-400.

Fang Ji-chao; Zhang Jin-ru; Xue-Qin-zhong; Wu Guang-nan. Studies on Specific Proteins of Somatic Embryogenesis in Oryz Sativa L. subsp. Indica[J]. Acta Agron Sin, 1994, 20(04): 395-400.

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