旱稻原生质体培养和植株再生的研究

作物学报 ›› 1994, Vol. 20 ›› Issue (04): 419-425.

旱稻原生质体培养和植株再生的研究

鲁雪华;刘中柱;林永烈;林大添;丁舒敏

福建省农业科学院，福建福州，350003

收稿日期:1992-04-28 修回日期:1993-03-28 出版日期:1994-07-12 网络出版日期:1994-07-12
通讯作者: 鲁雪华

Studies on Protoplast Culture of Upland Rice and Plant Regeneration

Lu Xue-hua; Liu Zhong-zhu; Lin Yong-lie; Lin Da-tian; Ding Shu-ming

Fujian Academy of Agricultural Sciences, Fuzhou,350003

Received:1992-04-28 Revised:1993-03-28 Published:1994-07-12 Published online:1994-07-12
Contact: Lu Xue-hua

摘要/Abstract

摘要： 旱稻仿野生87-707品系具有多年生趋势，是我院研究多年生旱稻的一个材料。我们用它进行原生质体培养，想为研究多年生旱稻基因改良打下基础。现用该品系的成熟胚诱导的愈伤组织，在含有氨基酸丰富的AA培养基中进行细胞悬浮培养。取该细胞悬浮培养物游离的原生质体，用琼脂糖包埋于改良的KM8P培养基中，发生了持续分裂。经一系

关键词: 旱稻，原生质体培养，渗透压，植株再生，琼脂糖包理培养，脱落酸

Abstract: Fang Ye 87-707 species of upland rice has the potential of perennial character, and thus serves as a material of the perennial upland rice in our Academy. We use it for protoplast culture and hope it lay a basis for studying genetic improvement of perennial upland rice. By suing the callus induction

Key words: Upland rice, Protoplast cultivation, Osmotic pressure, Plant regeneration, Agarose-embedding cultivation, ABA.

鲁雪华;刘中柱;林永烈;林大添;丁舒敏. 旱稻原生质体培养和植株再生的研究[J]. 作物学报, 1994, 20(04): 419-425.

Lu Xue-hua; Liu Zhong-zhu; Lin Yong-lie; Lin Da-tian; Ding Shu-ming. Studies on Protoplast Culture of Upland Rice and Plant Regeneration[J]. Acta Agron Sin, 1994, 20(04): 419-425.

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