磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化

作物学报 ›› 2003, Vol. 29 ›› Issue (04): 545-550.

磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化

王振英;程罗根;陈宏;彭永康

天津师范大学生物系,天津,300074

收稿日期:2002-05-16 修回日期:2002-12-03 出版日期:2003-07-12 网络出版日期:2003-07-12
通讯作者: 王振英

Induction of Abnormal Mitosis and Changes in Protein Compositions after Treatment of Root Meristems with Phosphoric Amide Herbicide APM in Allium

Wang Zhenying;Chen Luogen;Chen Hong;Peng Yongkang

Received:2002-05-16 Revised:2002-12-03 Published:2003-07-12 Published online:2003-07-12
Contact: Wang Zhenying

摘要/Abstract

摘要： 研究了不同浓度APM(4～6 μmol/L)和不同处理时间(4～6 h)对洋葱根尖细胞有丝分裂的影响.结果表明,当用5.6 μmol/L APM处理洋葱分生组织16 h后,中期细胞有丝分裂指数(Met.Ⅰ)从0.8(对照)分别提高至5.0、5.2.结果也同时表明,当APM浓度超过4 μmol/L时,严重影响细胞有丝分裂.在根尖细胞中,多极分裂细胞(尤其是3～4级),中期染色

关键词: 异常有丝分裂, 多级纺锤丝, APM, 2D-PAGE, 洋葱

Abstract: The effect of Amiprophose-methyl (APM) on mitotic cells of the root meristems of Allium cepa was studied at a range of concentrations (4-6 μmol/L), length of treatments (4-16 h). The results showed that the mitosis metaphase index (Met. I ) could be obviously improved from 0.8 (in control) to 5.0 o

Key words: Abnormal mitosis, Multipolar spindle, APM, 2D PAGE, Allium cepa

中图分类号:

S633

王振英;程罗根;陈宏;彭永康. 磷酰胺除草剂APM诱导洋葱根尖分生组织细胞异常有丝分裂和蛋白质组分变化[J]. 作物学报, 2003, 29(04): 545-550.

Wang Zhenying;Chen Luogen;Chen Hong;Peng Yongkang. Induction of Abnormal Mitosis and Changes in Protein Compositions after Treatment of Root Meristems with Phosphoric Amide Herbicide APM in Allium[J]. Acta Agron Sin, 2003, 29(04): 545-550.

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