关键词: 非组培转化, 花粉介导, 子房注射, 萌动种胚法

Abstract: At present, high velocity microprojectile and Agrobacterium tumeficiens infection are two main methods to transform cereal such as wheat and maize. The former is not only expensive, but also with low transgenic and high infertile frequency, and the later also takes immature embryo as the object, spending much time in tissue culture, and is with low transgenic frequency too. Although acetosyringone has been introduced into Agrobacterium tumeficiens infection, the transgenic frequency is still not high. At the same time, all these transgenic experiments can only be done in instrumented laboratory by few scientists. In this study, three non-tissue culture methods were used to transform foreign bar gene into maize. T₁ seeds were identified by Basta resistance, RCR amplification and Southern blotting, T₂ genetic rate and phenotype, in order to select a simple highly effective transgenic approach.

Key words: Non-tissue culture transformation method, Pollen mediation, Co-cultivation of germinated embryos with Agrobacterium tumefaciens, Ovary injection