马铃薯X病毒25 kD运动蛋白基因和外壳蛋白基因介导的抗病性研究

作物学报 ›› 2005, Vol. 31 ›› Issue (07): 827-832.

马铃薯X病毒25 kD运动蛋白基因和外壳蛋白基因介导的抗病性研究

刘晓玲;宋云枝;刘红梅;温孚江;朱常香;白庆荣

山东农业大学植物保护学院，山东泰安 271018

收稿日期:2004-09-03 修回日期:2004-12-29 出版日期:2005-07-12 网络出版日期:2005-07-12
通讯作者: 温孚江

Virus Resistance Mediated by the cDNAs Encoding for the Movement and Coat Proteins of Potato Virus X

LIU Xiao-Ling;SONG Yun-Zhi;LIU Hong-Mei;WEN Fu-Jiang;ZHU Chang-Xiang;BAI Qing-Rong

College of Plant Protection, Shandong Agricultural University, Tai’an 271018, Shandong

Received:2004-09-03 Revised:2004-12-29 Published:2005-07-12 Published online:2005-07-12
Contact: WEN Fu-Jiang

摘要/Abstract

摘要： 以马铃薯X病毒（potato virus X，PVX）的RNA为模板，应用反转录-聚合酶链式反应（RT-PCR）方法分别扩增出长度为681 bp的非翻译马铃薯X病毒25 kD运动蛋白基因（PVX-p25）和长度为714 bp的非翻译马铃薯X病毒外壳蛋白基因（PVX-CP）。并分别构建植物表达载体pROKⅡ-p25和pROKⅡ-CP。利用农杆菌介导方法转化烟草NC89。经卡那霉

关键词: 马铃薯X病毒, 运动蛋白, 外壳蛋白, RNA介导的病毒抗性

Abstract: According to the published nucleotide sequence of potato virus X （PVX）, the non-translational movement protein gene (681 bp in length) and the non-translational PVX coat protein (CP) gene (714 bp in length) of PVX were synthesized by reverse transcription-polymerase chain reaction (RT-PCR). The s

Key words: Potato virus X, Movement protein, Coat protein, RNA-mediated virus resistance

中图分类号:

Q943.2

刘晓玲;宋云枝;刘红梅;温孚江;朱常香;白庆荣. 马铃薯X病毒25 kD运动蛋白基因和外壳蛋白基因介导的抗病性研究[J]. 作物学报, 2005, 31(07): 827-832.

LIU Xiao-Ling;SONG Yun-Zhi;LIU Hong-Mei;WEN Fu-Jiang;ZHU Chang-Xiang;BAI Qing-Rong. Virus Resistance Mediated by the cDNAs Encoding for the Movement and Coat Proteins of Potato Virus X[J]. Acta Agron Sin, 2005, 31(07): 827-832.

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