欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1720-1726.doi: 10.3724/SP.J.1006.2013.01720

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗小麦黄矮病相关蛋白激酶TiDPK1与BYDV外壳蛋白的互作

汪信东,陈亮,张增艳*   

  1. 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程 / 农业部麦类生物学与遗传育种重点实验室, 北京 100081
  • 收稿日期:2013-02-05 修回日期:2013-04-22 出版日期:2013-10-12 网络出版日期:2013-08-01
  • 通讯作者: 张增艳, E-mail: zhangzengyan@ caas.cn, Tel: 010-82108781
  • 基金资助:

    本研究国家高技术研究发展计划(863计划)项目(2012AA10A309 and 2006AA100102)资助。

Interaction between Wheat Resistance-related Kinase TiDPK1 and BYDV Coat Protein

WANG Xin-Dong,CHEN Liang,ZHANG Zeng-Yan*   

  1. National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-02-05 Revised:2013-04-22 Published:2013-10-12 Published online:2013-08-01
  • Contact: 张增艳, E-mail: zhangzengyan@ caas.cn, Tel: 010-82108781

摘要:

小麦黄矮病是由大麦黄矮病毒(Barley yellow dwarf virus, BYDV)引起的小麦重要病毒病。分离于小麦中间偃麦草易位系的蛋白激酶编码基因TiDPK1, 是一个抗小麦黄矮病相关基因。本文报道利用酵母双杂交技术和双分子荧光互补技术对TiDPK1BYDV外壳蛋白(coat protein, CP)互作的研究结果。酵母双杂交分析结果表明, TiDPK1能够与BYDV-GAV-PAV株系的CP互作, 双分子荧光互补分析结果进一步表明, TiDPK1可与BYDVCP互作、产生双分子荧光互补信号, 说明TiDPK1确可与BYDV CP相互作用, 该结果对了解TiDPK1在小麦抗BYDV反应机制具有一定意义。

关键词: 小麦, 蛋白激酶TiDPK1, 大麦黄矮病毒外壳蛋白, 酵母双杂交, 双分子荧光互补, 蛋白互作

Abstract:

Yellow dwarf virus disease is one of the important diseases of wheat (Tritium aestivum L.) worldwide. It is caused by Barley yellow dwarf virus (BYDV) that is vectored by aphids. A kinase protein encoding gene TiDPK1, which is derived from Thinopyrum intermedium, is an important gene involved in BYDV resistance in wheat-T. intermedium translocation lines. In this study, we used yeast two-hybrid and bimolecular fluorescence complementation assays to explore the relationship between TiDPK1 and coat protein (CP) of BYDV (BYDV-CP). The results proved that the protein TiDPK1 interacted with BYDV-CP, which may offer an insight to the resistance mechanism of TiDPK1.

Key words: Wheat, Protein kinase TiDPK1, Coat protein of Barley yellow dwarf virus, Yeast two-hybrid, Bimolecular fluorescence complementation, Protein–protein interaction

[1]Zhang Z Y, Lin Z S, Xin Z Y. Research progress in BYDV resistance genes derived from wheat and its wild relatives. J Genet Genomics, 2009, 36: 567–573



[2]Singh R P. Genetic association of gene Bdv1 for tolerance to barley yellow dwarf virus with gene Lr34 and Yr18 for adult plant resistance to rusts in bread wheat. Plant Dis, 1993, 77: 1103–1106



[3]Xin Z Y, Xu H J, Chen X, Lin Z S, Zhou G H, Qian Y T, Cheng Z M, Larkin P J., Banks P, Appels R, Glarke B, Brettell R S I. Development of common wheat germplasm resistant to Barley yellow dwarf virus by biotechnology. Sci China (Sci B), 1991, 34(9): 1055–1062



[4]Sun S C. The approach and methods of breeding new varieties and new species from Agrotriticum hybrids. Acta Agron Sin (作物学报), 1981, 7(1): 51–55 (in Chinese with English abstract)



[5]Sharma H, Ohm H, Perry K L. Registration of Barley yellow dwarf virus resistant wheat germplasm line P29. Crop Sci, 1997, 37: 1032–1033



[6]Zhang Z, Xin Z, Ma Y, Chen X, Xu Q, Lin Z. 1999, Mapping of a BYDV resistance gene from Thinopyrum intermedium in wheat background by molecular markers. Sci China C (Life Sci.) 42: 663–668



[7]Milller W A, Rasochova L. Barley yellow dwarf viruses. Annu Rev Phytopathol, 1997, 35: 167–190



[8]Jarošová J, Chrpová J, Šíp V, Kundu J K. A comparative study of the Barley yellow dwarf virus species PAV and PAS: distribution, accumulation and host resistance. Plant Pathol, 2013, 62: 436–443



[9]Kvarnheden A. Viruses of field crops—an overview. Risk assessment/risk management, forecasting pests and diseases of field crops in a changing climate-control strategies for pests, diseases and weeds. In: NJF Seminar, Sweden, 2011. 446: 53–58



[10]Zhou G H, Zhang S X, Rochow W F. Identifiaction of a barley yellow dwarf luteovirus strain transmitted by Macrosiphum avenae and Schizaphis gramium. Acta Phytopatho Sin (植物病理学报), 1986, 16: 17–22 (in Chinese)



[11]Ueng P P, Vincent J R, Kawata E E, Lei C-H, Lister R M, Larkins B A. Nucleotide sequence analysis of the genomes of the MAV-PS1 and P-PAV isolates of Barley yellow dwarf virus. J Gen Virol, 1992, 73: 487–492



[12]Wu B L, Alexandra L B, Liu Y, Zhou G H, Wang X F, Elena S F. Dynamics of molecular evolution and phylogeography of Barley yellow dwarf virus-PAV. PLoS One, 2011, 6: e16896



[13]Vincent J R, Lister R M, Larkins B A. Nucleotide sequence analysis and genomic organization of the NY-RPV isolate of Barley yellow dwarf virus. J Gen Virol, 1991, 72: 2347–2355



[14]Jin Z B, Wang X F, Chang S, Zhou G H. The complete nucleotide sequence and its organization of the genome of Barley yellow dwarf virus-GAV. Sci. China Ser C ( Life Sci), 2004, 47: 175–182



[15]Zhang W W, Cheng Z M, Xu L, Wu M S, Waterhouse P, Zhou G H, Li S F. The complete nucleotide sequence of the barley yellow dwarf GPV isolate from China shows that it is a new member of the genus Polerovirus. Arch Virol, 2009, 154: 1125–1128



[16]Liu Y, Zhai H, Zhao K, Wu B B, Wang X F. Two suppressors of RNA silencing encoded by cereal-infecting members of the family Lutroviridae. J Gen Virol, 2012, 93: 1825–1830



[17]Song W Y, Wang G L, Chen L L, Kim H S, Pi L Y, Holsten T, Gardner, J, Wang B, Zhai W X, Zhu L H. A receptor kinase-like protein encoded by the rice disease resistance gene, Xa21. Science, 1995, 270: 1804–1806



[18]Zhou J M, Tang X Y, Martin G B. The Pto kinase conferring resistance to tomato bacterial speck disease interacts with proteins that bind a cis-element of pathogenesis-related genes. EMBO J, 1997, 16: 3207–3218



[19]Cao A, Xing L, Wang X, Yang X, Wang W, Sun Y, Qian C, Ni J, Chen Y, Liu D, Wang X E , Chen P. Serine/threonine kinase gene Stpk-V, a key member of powdery mildew resistance gene Pm21, confers powdery mildew resistance in wheat. Proc Natl Acad Sci USA, 2011, 108: 7727–7732



[20]Bendixen C, Gangloff S, Rothstein R. A yeast mating-selection scheme for detection of protein–protein interactions. Nucl Acids Res, 1994, 22: 1778–1779



[21]Hu C D, Chinenov Y, Kerppolal T. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Mol Cell, 2002, 9: 789–798



[22]Ito H, Fukuda Y, Murata K, Kimura A. Transformation of intact yeast cells treated with alkali cations. J Bacteriol, 1983, 162: 1142–1150



[23]Jones J D, Dangl J L. The plant immune system. Nature, 2006, 444: 323–332



[24]Stephen T, Chisholm, Gitta C, Brad D, Brain J. Host-microbe interactions: shaping the evolution of the plant immune response. Cell, 2006, 124: 803–814



[25]Deslandes L, Olivier J, Peeters N, Dong X F, Khounlotham M, Boucher C, Somssich I, Genin S, Marcos Y. Physical interaction between RRS1-R, a protein conferring resistance to bacterial wilt, and PopP2, a type III effector targeted to the plant nucleus. Proc Natl Acad Sci USA, 2003, 100: 8024–8029



[26]Mackey D, Holt B F, Wiig A, Dangl J L. RIN4 interacts with Pseudomonas syringae type III effector molecules and is required for RPM1-mediated disease resistance in Arabidopsis. Cell, 2002, 108: 743–754



[27]Kodama Y, Hu C D. Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. BioTechniques, 2012, 53: 285–298



[28]Bar M, Sharfman M, Ron M, Avni A. BAK1 is required for the attenuation of ethylene-inducing xylanase (Eix)-induced defense responses by the decoy receptor LeEix1. Plant J, 2000, 63: 791–800



[29]Burch-Smith T M, Schiff M, Caplan J L, Tsao J, Czymmek K, Dinesh-Kumar S P. A novel role for the TIR domain in association with pathogen-derived elicitors. PLoS Biol, 2007, 5: e68



[30]Wang W M, Ma X F, Zhang Y, Luo M C, Wang G L, Bellizzi M, Xiong X Y, Xiao S Y. PAPP2C interacts with the atypical disease resistance protein RPW8.2 and negatively regulates salicylic acid-dependent defense responses in Arabidopsis. Mol Plant, 2012, 5: 1125–1137

[1] 崔致远, 秦晨展, 刘星雨, 张海, 曾康, 黄国强, 徐景升. 甘蔗类四跨膜蛋白ScTSPAN18与6K2互作应答SCMV侵染研究[J]. 作物学报, 2026, 52(6): 1618-1630.
[2] 翟胜男, 曹新有, 李豪圣, 李吉虎, 李法计, 刘金栋, 夏先春, 吕莹莹, 马瑞峰, 王颖, 耿洪伟, 刘建军. 小麦Pod-A1Pod-D1Pod-2D位点等位变异对籽粒过氧化物酶活性的遗传效应分析[J]. 作物学报, 2026, 52(6): 1593-1603.
[3] 习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617.
[4] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[5] 胡川, 赵凯男, 黄修利, 吴金芝, 任开明, 王贺正, 付国占, 黄明, 李友军. 一次灌溉下耕作方式和氮肥用量对旱地小麦产量和品质的影响[J]. 作物学报, 2026, 52(6): 1830-1846.
[6] 陈雪燕, 何华川, 李政嘉, 董新盼, 李藕琪, 刘小云, 李丹萍, 陈志伟, 刘国霞, 吕胜源, 吴印莹, 赵振东, 曹新有, 万何平. 水培盐碱复合胁迫下‘济麦60’苗期根系有机酸分泌动态变化及其转录调控机制[J]. 作物学报, 2026, 52(6): 1859-1875.
[7] 左同鸿, 张贺翠, 曾静, 朱利泉. 甘蓝自交不亲和相关基因BoPUB3L的克隆与表达分析[J]. 作物学报, 2026, 52(6): 1698-1710.
[8] 高沛阳, 李瑾璇, 董宇奎, 石玉, 张振, 张永丽. 测墒补灌下小麦分蘖发生和成穗对施氮量的响应[J]. 作物学报, 2026, 52(6): 1847-1858.
[9] 张献丰, 郭利建, 李康春, 孔斌雪, 刘玉芳, 车卓, 杨德龙. 小麦ABHD6基因家族鉴定与粒重功能标记开发[J]. 作物学报, 2026, 52(6): 1711-1727.
[10] 王壮壮, 武紫君, 张永新, 张芯源, 袁丽雪, 陈如雪, 刘世举, 段剑钊, 冯伟, 王同朝, 王永华. 豫东南黏壤潮土区水氮优化协同提高冬小麦产量和氮素利用效率[J]. 作物学报, 2026, 52(5): 1501-1521.
[11] 何万龙, 耿洪伟, 张飞飞, 米克热阿依·阿巴白克热, 罗紫洋, 李鹏程, 周钊宇, 程宇坤. 基于深度学习的小麦重要病害图像识别系统的研究[J]. 作物学报, 2026, 52(5): 1401-1417.
[12] 张振, 冯连杰, 石玉, 于振文, 张永丽. 节水补灌下不同穗型小麦产量形成差异研究[J]. 作物学报, 2026, 52(5): 1522-1535.
[13] 侯思宇, 王国璀, 韦金贵, 谢玮欣, 殷文, 樊志龙, 柴强, 胡发龙. 绿肥配施化学氮肥对西北干旱灌区小麦干物质积累及产量形成的影响[J]. 作物学报, 2026, 52(4): 1208-1219.
[14] 尚云秋, 赵竹, 陈欢, 丁永刚, 乔玉强, 李玮, 张向前, 曹承富, 杜世州. 长期定位耕作方式对雨养小麦籽粒灌浆和产量形成的影响[J]. 作物学报, 2026, 52(4): 1236-1250.
[15] 乔宇馨, 李程越, 康晓玉, 张鑫琪, 贾绍辉, 刘倩, 曹亚丽, 史鑫蕊, 郝兴宇, 李萍. 基于APSIM模型的长期免耕秸秆覆盖对旱地小麦增产效应研究[J]. 作物学报, 2026, 52(4): 1181-1192.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!