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作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1727-1738.doi: 10.3724/SP.J.1006.2013.01727

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米再生相关基因ZmLEC1的序列变异及其与胚性愈伤组织形成能力的关联分析

李钊1,2,张登峰2,孙永华2,吴迅2,李永祥2,石云素2,宋燕春2,杨德光1,*,王天宇2,黎裕2,*   

  1. 1 东北农业大学农学院,黑龙江哈尔滨 150030;2 中国农业科学院作物科学研究所,北京 100081
  • 收稿日期:2013-01-28 修回日期:2013-06-02 出版日期:2013-10-12 网络出版日期:2013-08-01
  • 通讯作者: 杨德光, E-mail: ydgl@tom.com; 黎裕, E-mail: liyu03@caas.cn, Tel: 010-62131196
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2011ZX08010-004)资助。

Sequence Diversity of ZmLEC1 and Association Analysis of Embryogenic calli Formation Ability in Maize

LI Zhao1,2,ZHANG Deng-Feng2,SUN Yong-Hua2,WU Xun2,LI Yong-Xiang2,SHI Yun-Su2,SONG Yan-Chun2,YANG De-Guang1,*,WANG Tian-Yu2,LI Yu2,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China;2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-28 Revised:2013-06-02 Published:2013-10-12 Published online:2013-08-01
  • Contact: 杨德光, E-mail: ydgl@tom.com; 黎裕, E-mail: liyu03@caas.cn, Tel: 010-62131196

摘要:

95份玉米微核心种质和3份常用玉米转基因受体材料(A188HiII和综31)组成的关联作图群体,对玉米再生相关候选基因ZmLEC1进行序列变异分析,并利用候选基因关联分析策略揭示该基因与胚性愈伤组织形成能力的关系,发掘提高胚性愈伤组织形成能力的有益等位变异。结果表明,不同材料之间的幼胚胚性愈伤组织形成能力和再生能力有显著差异,其中粤267-1-1诱导的愈伤组织和国际上普遍利用的HiII极为相似,胚性愈伤组织率达到98.48%,可以用于幼胚的遗传转化。ZmLEC1基因多态性分析表明,在852 bp编码区内共发现33SNPs9INDELsLD衰减距离为300 bp (R2=0.1)ZmLEC1基因中4个多态性位点与胚性愈伤组织形成能力存在显著关联。

关键词: 玉米, ZmLEC1, 胚性愈伤组织, 关联分析

Abstract:

A maize association mapping population consisted of a mini core collection of ninety-five maize inbred lines and three elite maize accessions (A188, HiII, and Zong 31)usually for genetic transformation was used to analyze the sequence diversity and linkage disequilibrium (LD) of ZmLEC1, a candidate gene of regeneration ability in maize. A candidate gene association strategy was used to reveal the relationship between this gene and embryogenic calli formation ability and discover favorable alleles and genotypes enhancing the embryogenic calli formation ability. The results showed that there existed significant differences in abilities of embryogenic calli formation and regeneration among these accessions. The calli induced from Yue267-1-1 were very similar to those of HiII, the popularly used genotype in maize transformation. Yue267-1-1 had the highest ability of embryogenic calli formation and regeneration and could be a new germplasm for immature embryo-based genetic transformation. The result of sequence polymorphism analysis of ZmLEC1 showed that there were thirty-three SNPs and nine InDels in the coding region of 852 bps. The LD between all of the informative polymorphisms decayed rapidly to about 300 bp at R2=0.1. Totally four polymorphic sites in the ZmLEC1 gene were significantly associated with embryogenetic calli formation ability.

Key words: Zea mays, ZmLEC1, Embryogenetic callus, Association analysis

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