作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1727-1738.doi: 10.3724/SP.J.1006.2013.01727
李钊1,2,张登峰2,孙永华2,吴迅2,李永祥2,石云素2,宋燕春2,杨德光1,*,王天宇2,黎裕2,*
LI Zhao1,2,ZHANG Deng-Feng2,SUN Yong-Hua2,WU Xun2,LI Yong-Xiang2,SHI Yun-Su2,SONG Yan-Chun2,YANG De-Guang1,*,WANG Tian-Yu2,LI Yu2,*
摘要:
以95份玉米微核心种质和3份常用玉米转基因受体材料(A188、HiII和综31)组成的关联作图群体,对玉米再生相关候选基因ZmLEC1进行序列变异分析,并利用候选基因关联分析策略揭示该基因与胚性愈伤组织形成能力的关系,发掘提高胚性愈伤组织形成能力的有益等位变异。结果表明,不同材料之间的幼胚胚性愈伤组织形成能力和再生能力有显著差异,其中粤267-1-1诱导的愈伤组织和国际上普遍利用的HiII极为相似,胚性愈伤组织率达到98.48%,可以用于幼胚的遗传转化。ZmLEC1基因多态性分析表明,在852 bp编码区内共发现33个SNPs和9个INDELs,LD衰减距离为300 bp (R2=0.1),ZmLEC1基因中4个多态性位点与胚性愈伤组织形成能力存在显著关联。
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