欢迎访问作物学报,今天是

作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1727-1738.doi: 10.3724/SP.J.1006.2013.01727

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

玉米再生相关基因ZmLEC1的序列变异及其与胚性愈伤组织形成能力的关联分析

李钊1,2,张登峰2,孙永华2,吴迅2,李永祥2,石云素2,宋燕春2,杨德光1,*,王天宇2,黎裕2,*   

  1. 1 东北农业大学农学院,黑龙江哈尔滨 150030;2 中国农业科学院作物科学研究所,北京 100081
  • 收稿日期:2013-01-28 修回日期:2013-06-02 出版日期:2013-10-12 网络出版日期:2013-08-01
  • 通讯作者: 杨德光, E-mail: ydgl@tom.com; 黎裕, E-mail: liyu03@caas.cn, Tel: 010-62131196
  • 基金资助:

    本研究由国家转基因生物新品种培育重大专项(2011ZX08010-004)资助。

Sequence Diversity of ZmLEC1 and Association Analysis of Embryogenic calli Formation Ability in Maize

LI Zhao1,2,ZHANG Deng-Feng2,SUN Yong-Hua2,WU Xun2,LI Yong-Xiang2,SHI Yun-Su2,SONG Yan-Chun2,YANG De-Guang1,*,WANG Tian-Yu2,LI Yu2,*   

  1. 1 College of Agriculture, Northeast Agricultural University, Harbin 150030, China;2 Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2013-01-28 Revised:2013-06-02 Published:2013-10-12 Published online:2013-08-01
  • Contact: 杨德光, E-mail: ydgl@tom.com; 黎裕, E-mail: liyu03@caas.cn, Tel: 010-62131196

摘要:

95份玉米微核心种质和3份常用玉米转基因受体材料(A188HiII和综31)组成的关联作图群体,对玉米再生相关候选基因ZmLEC1进行序列变异分析,并利用候选基因关联分析策略揭示该基因与胚性愈伤组织形成能力的关系,发掘提高胚性愈伤组织形成能力的有益等位变异。结果表明,不同材料之间的幼胚胚性愈伤组织形成能力和再生能力有显著差异,其中粤267-1-1诱导的愈伤组织和国际上普遍利用的HiII极为相似,胚性愈伤组织率达到98.48%,可以用于幼胚的遗传转化。ZmLEC1基因多态性分析表明,在852 bp编码区内共发现33SNPs9INDELsLD衰减距离为300 bp (R2=0.1)ZmLEC1基因中4个多态性位点与胚性愈伤组织形成能力存在显著关联。

关键词: 玉米, ZmLEC1, 胚性愈伤组织, 关联分析

Abstract:

A maize association mapping population consisted of a mini core collection of ninety-five maize inbred lines and three elite maize accessions (A188, HiII, and Zong 31)usually for genetic transformation was used to analyze the sequence diversity and linkage disequilibrium (LD) of ZmLEC1, a candidate gene of regeneration ability in maize. A candidate gene association strategy was used to reveal the relationship between this gene and embryogenic calli formation ability and discover favorable alleles and genotypes enhancing the embryogenic calli formation ability. The results showed that there existed significant differences in abilities of embryogenic calli formation and regeneration among these accessions. The calli induced from Yue267-1-1 were very similar to those of HiII, the popularly used genotype in maize transformation. Yue267-1-1 had the highest ability of embryogenic calli formation and regeneration and could be a new germplasm for immature embryo-based genetic transformation. The result of sequence polymorphism analysis of ZmLEC1 showed that there were thirty-three SNPs and nine InDels in the coding region of 852 bps. The LD between all of the informative polymorphisms decayed rapidly to about 300 bp at R2=0.1. Totally four polymorphic sites in the ZmLEC1 gene were significantly associated with embryogenetic calli formation ability.

Key words: Zea mays, ZmLEC1, Embryogenetic callus, Association analysis

[1]Hecht V, Vielle-Calzada J P, Hartog M V, Schmidt E D L, Boutilier K, Grossniklaus U, de Vries S C. The Arabidopsis SOMATIC EMBRYOGENESIS KINASE 1 is expressed in developing ovules and embryos and enhances embryogenic competence in culture. Plant Physiol, 2001, 127: 803–816



[2]Nishimura A, Ashikari M, Lin S Y, Takashi T, Angeles E R, Yamamoto T, Matsuoka M. Isolation of a rice regeneration quantitative trait loci gene and its application to transformation systems. Proc Natl Acad Sci USA, 2005, 102:11940–11944



[3]Ozawa K, Kawahigashi H. Positional cloning of the nitrite reductase gene associated with good growth and regeneration ability of calli and establishment of a new selection system for Agrobacterium-mediated transformation in rice (Oryza sativa L.). Plant Sci, 2006, 170:384–393



[4]Tromas A, Paponov I, Perrot-Rechenmann C. AUXIN BINDING PROTEIN 1: functional and evolutionary aspects. Trends Plant Sci, 2010, 15:436–446



[5]Sauter M, Wiegen P, Lörz H, Kranz E. Cell cycle regulatory genes from maize are differentially controlled during fertilization and first embryonic cell division. Sexual Plant Reprod, 1998, 11: 41–48



[6]Meinke D W,Franzmann L H,Nickle T C,Yeung E C.Leafy cotyledon mutants of Arabidopsis. Plant Cell, 1994, 6:1049–1064



[7]Lotan T, Ohto M, Yee K M, West M A L, Lo R, Kwong R W, Yamagishi K, Fischer R L, Goldberg R B, Harada J J. Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells. Cell, 1998, 93:1195–1205



[8]Zhang S, Wong L, Meng L, Lemaux P G. Similarity of expression patterns of knotted1 and ZmLEC1 during somatic and zygotic embryogenesis in maize ( Zea mays L.). Planta, 2002, 215:191–194



[9]Duncan D R, Williams M E, Zehr B E, Widholm J M. The production of callus capable of plant regeneration from immature embryos of numerous Zea mays genotypes. Planta. 1985, 165:322–332



[10]Bolibok H, Rakoczy-Trojanowska M. Genetic mapping of QTLs for tissue culture response in plants. Euphytica, 2006, 149: 73–83_



[11]Armstrong C L , Romero-Severson J, Hodges T K. Improved tissue culture response of an elite maize inbred through back cross breeding , and identification of chromosomal regions important f or regene ration by RFLP analysis. Theor Appl Genet, 1992, 84: 755–762



[12]Krakowsky M D , Lee M, Garay L. Quantitative trait loci for callus initiation in maize (Zea mays L.). Theor Appl Genet, 2006, 113: 821–830 _



[13]Zhang H-W(张红伟), Liu Y-J(刘亚娟), Guo X-L(郭晓琳), Zhang F(张峰),Li J-S(李建生), Chen G(陈刚),Sun D-F(孙东发), Tan Z-B(谭振波). QTL mapping for callus induction and plant regeneration in maize immature embryos. Acta Agrono Sin(作物学报), 2006, 32(3): 385–389 (in Chinese with English abstract)



[14]Ye X-G(叶兴国), She M-Y(佘茂云), Wang K(王轲), Du L-P(杜丽璞), XU H-J(徐惠君). Identification, cloning, and potential application of genes related to somatic embryogenesis in plant tissue culture. Acta Agrono Sin(作物学报), 2012, 38(2): 191–201(in Chinese with English abstract)



[15]Yang X-H(杨小红), Yan J-B(严建兵), Zheng Y-P(郑艳萍), Yu J-M(余建明), Li J-S(李建生). Reviews of association analysis for quantitative traits in plants. Acta Agrono Sin(作物学报), 2007, 33(4): 523–530(in Chinese with English abstract)



[16]Wang R-H(王荣焕), Wang T-Y(王天宇), Li Y(黎裕). Linkage disequilibrium in plant genomes. Hereditas(遗传), 2007, 29(11): 1317–1321(in Chinese with English abstract)



[17]Thornsberry J M, Goodman M M, Doebley J, Kresovich S, Nielsen D, Buckler E S. Dwarf8 polymorphisms associate with variation in flowering time. Nat Genet, 2001, 28: 286–289



[18]Palaisa K A, Morgante M, Williams M, Rafalski A. Contrasting effects of selection on sequence diversity and linkage disequilibrium at two phytoene synthase loci. Plant Cell, 2003, 15: 1795–1806



[19]Wilson L M, Whitt S R, Iba´n˜ez A M, Rocheford T R, Goodman M M, Buckler E S. Dissection of maize kernel composition and starch production by candidate gene association. Plant Cell, 2004, 16: 2719–2733



[20]Szalma S J, Buckler E S, Snook M E, McMullen M D. Association analysis of candidate genes for maysin and chlorogenic acid accumulation in maize silks. Theor Appl Genet, 2005, 110: 1324–1333



[21]Andersen J R, Zein I, Wenzel G, Krützfeldt B, Eder J, Ouzunova M, Lübberstedt T. High levels of linkage disequilibrium and associations with forage quality at a Phenylalanine Ammonia-Lyase locus in European maize (Zea mays L.) inbreds. Theor Appl Genet, 2006, 114: 307–319



[22]Yan J B, Brutnel T, Kandianis C B, Harjes C E, Bai L,Kim E H, Yang X H, Skinner D J, Fu Z Y, Mitchell S, Li Q, Fernandez M G S, Zaharieva M, Babu R,Fu Y, Palacios N, Li J S, DellaPenna D, Brutnell T, Buckler E S, Warburton M L, Rocheford T. Rare genetic variation at Zea mays crtRB1 increases carotene in maize grain. Nat Genet, 2010, 42: 322–327



[23]Wang R H, Yu Y T, Zhao J R, Shi Y S, Song Y C, Wang T Y, Li Y. Population structure and linkage disequilibrium of a mini core set of maize inbred lines in China. Theor Appl Genet, 2008, 117: 1141–1153 



[24]Ishida Y, Hiei Y, Komari T. Agrobacterium-mediated transformation of maize. Nat Protoc, 2007, 2: 1614–1621



[25]Armstrong C L, Green C E. Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline. Planta, 1985, 164: 207–214



[26]Thompson J D, Gibson T J, Plewniak F, Jeanmougin F, Higgins D G. The ClustalX windows interface: flexible strategies of multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 1997, 25: 4876–4882



[27]Rozas J, Sanchez-DelBarrio J C, Messeguer X. DnaSP, DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 2003, 19:2496–2497



[28] Bradbury P J, Zhang Z W, Kroon D E, Casstevens T M, Ramdoss Y, Buckler E S. TASSEL: software for association mapping of complex traits in diverse samples. Bioinform Appl Note, 2007, 23: 2633–2635 



[29] Zhao Z-Y, Gu W N, Cai T S, Tagliani L, Hondred D, Bond D, Schroeder S, Rudert M, Pierce D. High throughput genetic transformtion mediated by Agrobacterum trmefaciens in maize. Mol Breed, 2001, 8: 323–333



[30] Ishida Y, Saito H, Hiei Y, Komari T. Improved protocol for transformation of maize(Zea Maize L.) mediated by Agrobacterium tumefaciens. Plant Biotechnol, 2003, 20: 57–66



[31] Wang Z-Y(王章英). Isolation and Characterization of Maize Endosperm AGPase Mutants and Improving Maize Starch Content by Using Genetic Engineering. PhD Dissertation of China Agricultura University, 2006(in Chinese with English abstract)



[32] Liang G-D(梁广东), Di H(邸宏), Lu C-H(卢翠华), Zhang L(张林), Dong L(董玲), Wang Z-H(王振华), Jiang L-L(姜丽丽), Zhou Y(周羽). Study onimmature embryos regeneration of maize inbred lines. J Northeast Agric Univ(东北农业大学学报), 2010, 41(2): 11–14(in Chinese with English abstract)



[33] Hu Y-M(胡彦民), Tang J-H(汤继华), Liu Z-H(刘宗华), Ji H-Q(季洪强), Shi H-L(史红丽), Ji L-Y(季良越). Selection of the genotypes of high plant regeneration frequency from immature embryo calli in maize. Henan Sci(河南科学), 2004, 22(1): 63–66(in Chinese with English abstract)



[34] Wang H-N(王汉宁), Zhang J-W(张金文), Kong W-P(孔维萍), Feng Y-L(冯玉兰). Callus initiation and regeneration from immture embryos of maize. J Maize Sci(玉米科学), 2006, 14(5): 71–73(in Chinese with English abstract)



[35] Wu H(吴红), Xie S-Z(谢树章), Lin Q(林清), Lei K-R(雷开荣),  Qiu Z-G(邱正高), Zhang Y-Q(张亚勤), Wang N(王楠), Zhou Y-K(周幼昆). Study on callus induction and plantlet regeneration from immature embyro among different maize inbreds.Acta Agric Southwest (西南农业学报), 2012, 25(2): 385–389(in Chinese with English abstract)



[36] Mu G-Q(母贵琴), Pan G-T(潘光堂), Liu Y-Z(刘玉贞), Xia Y-L(夏燕莉). Preliminary study on maize genotypes and the establishment of embryogenic callus. J Sichuan Agric Univ(四川农业大学学报), 2003, 21(1): 13–17(in Chinese with English abstract)



[37] Ching A, Caldwell K S, Jung M, Dolan M, Smith O S, Tingey S, Morgante M, Rafalski A J. SNP frequency haplotype structure and linkage disequilibrium in elite maize inbred lines. BMC Genet, 2003, 3: 1–14



[38] Jung M, Ching A, Bhattramakki D, Dolan M, Tingey S, Morgante M, Rafalski A. Linkage disequilibrium and sequence diversity in a 500-kb region around the adh1 locus in elite maize germplasm. Theor Appl Genet, 2004, 109: 681–689



[39] Nordborg M. Linkage disequilibrium, gene trees and sel?ng: an ancestral recombination graph with partial self-fertilization. Genetics ,2000, 154: 923–929



[40] Remington D L, Thornsberry J M, Matsuoka Y, Wilson L M, Whitt S R, Doebley J, Kresovich S, Goodman M M, Buckler E S. Structure of linkage disequilibrium and phenotypic associations in the maize genome. Proc Natl Acad Sci USA, 2001, 98: 11479–11484



[41] Tenaillon M I, Sawkins M C, Long A D, Long R L, Doebley J F, Gaut B S. Patterns of DNA sequence polymorphism along chromosome 1 of maize (Zea mays ssp. mays L.). Proc Natl Acad Sci USA, 2001, 98: 9161–9166

[1] 习千辉, 徐梓瑗, 刘梦梦, 王宏艺, 郎凯琳, 井震海, 陈锋, 赵磊. 小麦籽粒铜含量的全基因组关联分析及候选基因预测[J]. 作物学报, 2026, 52(6): 1604-1617.
[2] 毛嘉琦, 黄朋雨, 赵佳佳, 郑兴卫, 武棒棒, 郝宇琼, 屈非, 刘成, 马朋涛, 郑军. 山西小麦品种白粉病抗性评价及抗病基因分子检测[J]. 作物学报, 2026, 52(6): 1669-1681.
[3] 刘恩波, 陈静, 李红星, 于宁宁, 任佰朝, 赵斌, 刘鹏, 张吉旺. 遮阴改变源-库平衡和调节碳水化合物代谢进而抑制夏玉米幼穗发育[J]. 作物学报, 2026, 52(6): 1891-1901.
[4] 梁进宇, 尹嘉德, 王红丽, 张国平, 侯慧芝, 董博, 马明生. 基于无人机高光谱和机器学习的旱地饲用玉米叶片氮含量估测[J]. 作物学报, 2026, 52(6): 1788-1801.
[5] 唐宽强, 李公允, 宋美毅, 赵雪, 常春玲. 大豆株高性状全基因组关联分析及预测模型构建[J]. 作物学报, 2026, 52(6): 1743-1756.
[6] 杨欣雨, 崔文涛, 迪力尼格尔·阿力木, 汪凯翔, 吴鹏昊, 任姣姣. 玉米穗上叶片数全基因组关联分析和全基因组选择[J]. 作物学报, 2026, 52(5): 1573-1590.
[7] 韩亚鑫, 何冠华, 张小琼, 张登峰, 李永祥, 刘旭洋, 王天宇, 黎裕, 邹华文, 李春辉. 基于RNA-Seq和BSA-Seq联合分析挖掘玉米侧根密度基因资源[J]. 作物学报, 2026, 52(5): 1341-1352.
[8] 闫安, 蒋昆炜, 王蓉圆, 田林, 张璐, 王韵, 徐建龙. 水稻剑叶小维管束数基因SVN7的鉴定与克隆[J]. 作物学报, 2026, 52(5): 1364-1372.
[9] 孙淑凤, 许振南, 黄嘉鑫, 翁建峰, 李新海. 玉米MAPK家族全基因组鉴定及其对拟轮枝镰孢菌感染的响应[J]. 作物学报, 2026, 52(5): 1291-1308.
[10] 张宁宁, 滕雨菲, 任娜娜, 魏兴卓, 闫书豪, 樊可心, 王永宏, 陈文康, 张兴华, 朱万超, 徐淑兔, 薛吉全. 201份玉米自交系抗旱表型评价及可塑性分析[J]. 作物学报, 2026, 52(5): 1309-1325.
[11] 杨扬, 常诗惠, 田红丽, 易红梅, 王璐, 任洁, 范亚明, 刘亚维, 王凤格, 赵久然. 不同生态区国审玉米品种的遗传多样性分析[J]. 作物学报, 2026, 52(5): 1352-1364.
[12] 张鸿蓉, 王菲儿, 李盼, 仇海龙, 朱静, 赵连豪, 南运有, 何蔚, 樊志龙, 胡发龙, 柴强, 殷文. 减量20%灌水与25%有机肥替代化肥提高青贮玉米产量的光合特性[J]. 作物学报, 2026, 52(5): 1487-1500.
[13] 蔡宏玮, 于爱忠, 姜科强, 王鹏飞, 王玉珑, 霍建喆, 庞小能, 尹波, 尚永盼. 干旱灌区有机肥替代部分化肥促进甜玉米产量提升的关键机制[J]. 作物学报, 2026, 52(4): 1166-1180.
[14] 田红丽, 杨扬, 范亚明, 易红梅, 郭丹丹, 王凤格, 赵久然. 适于玉米品种鉴定的一套三等位变异SNP新型标记组合[J]. 作物学报, 2026, 52(4): 993-1005.
[15] 杨亚莉, 徐明睿, 马越飞, 海艺蕊, 刘凯栋, 刘万茂, 孙颖. 玉米根尖及整根响应缺铁的转录组比较研究[J]. 作物学报, 2026, 52(4): 1006-1021.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!