作物学报 ›› 2013, Vol. 39 ›› Issue (10): 1739-1745.doi: 10.3724/SP.J.1006.2013.01739
盖江涛,赵团结,李艳*,盖钧镒*
GAI Jiang-Tao,ZHAO Tuan-Jie,LI Yan*,GAI Jun-Yi*
摘要:
腺苷酸激酶(adenylate kinase,ADK)催化ATP+AMP⇔2ADP的可逆反应,是维持细胞能量动态平衡的关键酶,在植物中参与调节生长发育和逆境应答等过程。目前有关大豆ADK的研究还未见报道。本文通过RT-PCR方法,从耐盐大豆品种南农1138-2的叶中克隆到一个腺苷酸激酶基因,命名为GmADK。GmADK的编码区序列(coding DNA sequence, CDS)长804 bp,编码267个氨基酸。预测其蛋白结构含有典型的腺苷酸激酶功能域ATP-AMP(Ap5A)结合位点和AMP结合位点。蛋白序列比对和进化树分析表明,大豆与菜豆(Phaseolus vulgaris)和蒺藜苜蓿(Medicago truncatula)中的ADK序列相似性最高,亲缘关系最近。组织表达显示GmADK基因的表达量在大豆叶和根中高于茎中。荧光定量PCR分析表明GmADK的表达受盐胁迫的调节,且在耐盐(南农1138-2)和盐敏感(科丰1号)品种间存在差异。在叶和根中, 200 mmol L–1 NaCl处理6、12和24 h后,GmADK的表达量在盐敏感品种中比未处理对照有所降低,但在耐盐品种中却比未处理对照升高,因此推测GmADK可能参与大豆对盐胁迫的响应。
| [1]Tan Z-W(谭志文), Liu J(刘俊), Zhang X-F(张学芳), Meng F-G(孟凡国), Zhang Y-Z(张耀洲). Expression, purification and enzymatic characterization of adenylate kinase of Thermus thermophilus HB27 in Escherichia coli. J Southern Med Univ (南方医科大学学报), 2010, 30(1): 1–6 (in Chinese with English abstract)[2]Declerck P J, Muller M. Hydrogenosomal ATP:AMP phosphotransferase of Trichomonas vaginalis. Comp Biochem Physiol B, 1987, 88: 575–580[3]Chen B, Sysoeva T A, Chowdhury S, Guo L, Nixon B T. ADPase activity of recombinantly expressed thermotolerant ATPases may be caused by copurification of adenylate kinase of Escherichia coli. FEBS J, 2009, 276: 807–815[4]Goldberg R N, Tewari Y B, Bhat T N. Thermodynamics of enzyme-catalyzed reactions—a database for quantitative biochemistry. Bioinformatics, 2004, 20: 2874–2877[5]Pradet A, Raymond P. Adenine nucleotide ratios and adenylate energy charge in energy metabolism. Annu Rev Plant Biol, 1983, 34: 199–224[6]Atkinson D E. The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers. Biochemistry, 1968, 7: 4030–4034[7]Schlattner U, Wagner E, Greppin H, Bonzon M. Chloroplast adenylate kinase from tobacco: Purification and partial characterization. Phytochemistry, 1996, 42: 589–594[8]Ching T M, Crane J M. Adenylate energy pool and energy charge in maturing rape seeds. Plant Physiol, 1974, 54: 748–751[9]Raymond P, Pradet A. Stabilization of adenine nucleotide ratios at various values by an oxygen limitation of respiration in germinating lettuce (Lactuca sativa) seeds. Biochem J, 1980, 190: 39–44[10]Birkenhead K, Walker D, Foyer C. The intracellular distribution of adenylate kinase in the leaves of spinach, wheat and barley. Planta, 1982, 156: 171–175[11]Schlattner U, Wagner E, Greppin H, Bonzon M. Adenylate kinase in tobacco cell cultures: II. Variability and regulation of isoform activity patterns in different cell lines. J Plant Physiol, 1994, 144: 400–409[12]Kleczkowski L A, Randall D D. Equilibration of adenylates by maize leaf adenylate kinase: effects of magnesium on apparent and true equilibria. J Exp Bot, 1991, 42: 537–540[13]Regierer B, Fernie A R, Springer F, Perez-Melis A, Leisse A, Koehl K, Willmitzer L, Geigenberger P, Kossmann J. Starch content and yield increase as a result of altering adenylate pools in transgenic plants. Nat Biotechnol, 2002, 20: 1256–1260[14]Zancani M, Casolo V, Vianello A, Francesco Macr??. Involvement of apyrase in the regulation of the adenylate pool by adenylate kinase in plant mitochondria. Plant Sci, 2001, 161: 927–933[15]Kawai M, Uchimiya H. Biochemical properties of rice adenylate kinase and subcellular location in plant cells. Plant Mol Biol, 1995, 27: 943–951[16]Xu B-N(徐柏年), Zhang T-Y(张添元), Luo J-X(罗进贤), Wu Q(吴青), Wu J-X(吴江雪). Expression of ADK Gene and ATP Synthesis in Saccharomyces cerevisiae. Acta Sci Nat Univ Sunyatseni (中山大学学报?自然科学版), 2000, (5): 1–4 (in Chinese with English abstract)[17]Carrari F, Coll-Garcia D, Schauer N, Lytovchenko A, Palacios-Rojas N, Balbo I, Rosso M, Fernie A R. Deficiency of a plastidial adenylate kinase in Arabidopsis results in elevated photosynthetic amino acid biosynthesis and enhanced growth. Plant Physiol, 2005, 137: 70–82[18]Peterson T A, Nieman R H, Clark R A. Nucleotide Metabolism in Salt-Stressed Zea mays L. Root Tips: I. Adenine and Uridine Nucleotides. Plant Physiol, 1987, 85: 984–989[19]Gong P, Zhang J, Li H, Yang C, Zhang C, Zhang X, Khurram Z, Zhang Y, Wang T, Fei Z, Ye Z. Transcriptional profiles of drought-responsive genes in modulating transcription signal transduction, and biochemical pathways in tomato. J Exp Bot, 2010, 61: 3563–3575[20]Chen H-T(陈华涛), Chen X(陈新), Yu D-Y(喻德跃). Inheritance analysis and mapping quantitative trait loci (QTLs) associated with salt tolerance during seedling growth in soybean. Chin J Oil Crop Sci (中国油料作物学报), 2011, 33(3): 231–234 (in Chinese with English abstract)[21]Berry M B, Phillips G N Jr. Crystal structures of Bacillus stearothermophilus adenylate kinase with bound Ap5A, Mg2+ Ap5A, and Mn2+ Ap5A reveal an intermediate lid position and six coordinate octahedral geometry for bound Mg2+ and Mn2+. Proteins, 1998, 32: 276–288[22]Birkenhead K, Walker D, Foyer C H. The intracellular distribution of adenylate kinase in the leaves of spinach, wheat and barley. Planta, 1982, 156: 171–175[23]Hampp R, Goller M, Ziegler H. Adenylate levels, energy charge, and phosphorylation potential during dark-light and light-dark transition in chloroplasts, mitochondria, and cytosol of mesophyll protoplasts from Avena sativa L. Plant Physiol, 1982, 69: 448– 455[24]Stitt M, Lilley R M, Heldt H W. Adenine nucleotide levels in the cytosol, chloroplasts, and mitochondria of wheat leaf protoplasts. Plant Physiol, 1982, 70: 971–977[25]Lange P R, Geserick C, Tischendorf G, Zrenner R. Functions of chloroplastic adenylate kinases in Arabidopsis. Plant Physiol, 2008, 146: 492–504[26]Zhou S P, Wei S, Boone B, Levy S. Microarray analysis of genes affected by salt stress in tomato. Afr J Environ Sci Technol, 2007, 1: 014–026 |
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