作物学报 ›› 2022, Vol. 48 ›› Issue (3): 580-589.doi: 10.3724/SP.J.1006.2022.11015
付美玉1,2(), 熊宏春2, 周春云2, 郭会君2, 谢永盾2, 赵林姝2, 古佳玉2, 赵世荣2, 丁玉萍2, 徐延浩1,*(), 刘录祥2,*()
FU Mei-Yu1,2(), XIONG Hong-Chun2, ZHOU Chun-Yun2, GUO Hui-Jun2, XIE Yong-Dun2, ZHAO Lin-Shu2, GU Jia-Yu2, ZHAO Shi-Rong2, DING Yu-Ping2, XU Yan-Hao1,*(), LIU Lu-Xiang2,*()
摘要:
倒伏易引发小麦严重减产, 发掘和利用优异矮秆基因是培育高产抗倒伏小麦新品种的关键。本研究以京411 (WT)及其经EMS诱变获得的产量相关性状优良的矮秆突变体je0098为试验材料, 对其株高进行遗传分析, 结合外显子捕获测序和遗传连锁分析定位矮秆基因。3年田间株高数据统计分析表明, je0098与WT相比株高降低15 cm, 组织细胞学观察结果显示, je0098与WT相比节间细胞长度缩短18%, 暗示je0098的矮化是由于节间细胞长度变短所致; 赤霉素敏感性分析表明, je0098为赤霉素敏感型矮秆突变体。利用WT和je0098杂交构建的由344个单株组成的F2分离群体, 结合F2:3家系表型数据, 选取矮秆纯合和高秆单株构建混池, 对两亲本和子代混池分别进行外显子捕获测序, 在2D染色体上定位到一个具有降秆效应的数量性状位点(QTL)。结合全基因组重测序所得SNP位点, 在2D染色体开发了6个KASP分子标记, 对F2单株进行基因分型。利用QTL IciMapping作图软件构建遗传连锁图谱, 结合3年田间表型数据, 将矮秆基因定位在20.77~28.84 Mb区间内, 遗传距离为11.48 cM。本研究结果为突变体je0098矮秆基因的功能研究以及育种利用奠定了基础。
[1] |
Fan M, Shen J, Yuan L, Jiang R, Chen X, Davies W J, Zhang F. Improving crop productivity and resource use efficiency to ensure food security and environmental quality in China. J Exp Bot, 2012, 63:13-24.
doi: 10.1093/jxb/err248 |
[2] |
Wan J. Genetic Crop Improvement: a guarantee for sustainable agricultural production. Engineering, 2018, 4:431-432.
doi: 10.1016/j.eng.2018.07.019 |
[3] |
Peng J, Richards D E, Hartley N M, Murphy G P, Devos K M, Flintham J E, Beales J, Fish L J, Worland A J, Pelica F, Sudhakar D, Christou P, Snape J W, Gale M D, Harberd N P. ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature, 1999, 400:256-261.
doi: 10.1038/22307 |
[4] | 蒋梦婷, 渠慎春. DELLA蛋白在植物生长发育中的作用. 西北植物学报, 2018, 38:1952-1960. |
Jiang M T, Qu S C. DELLA and its functions in plant growth and development. Acta Bot Boreal-occident Sin, 2018, 38:1952-1960 (in Chinese with English abstract). | |
[5] |
Hauvermale A L, Ariizumi T, Steber C M. Gibberellin signaling: a theme and variations on DELLA repression. Plant Physiol, 2012, 160:83-92.
doi: 10.1104/pp.112.200956 pmid: 22843665 |
[6] |
Daviere J M, Achard P. A pivotal role of DELLAs in regulating multiple hormone signals. Mol Plant, 2016, 9:10-20.
doi: 10.1016/j.molp.2015.09.011 |
[7] |
Hedden P. The genes of the green revolution. Trends Genet, 2003, 19:5-9.
pmid: 12493241 |
[8] |
Würschum T, Langer S M, Longin C F. Genetic control of plant height in European winter wheat cultivars. Theor Appl Genet, 2015, 128:865-874.
doi: 10.1007/s00122-015-2476-2 pmid: 25687129 |
[9] |
Chen G, Zheng Q, Bao Y, Liu S, Wang H, Li X. Thinopyrum ponticum chromatin Thinopyrum ponticum chromatin. J Biosci, 2012, 37:149-155.
doi: 10.1007/s12038-011-9175-1 |
[10] |
Zhao K, Xiao J, Liu Y, Chen S, Yuan C, Cao A, You F M, Yang D, An S, Wang H, Wang X. Rht23 (5Dq') likely encodes a Q homeologue with pleiotropic effects on plant height and spike compactness. Theor Appl Genet, 2018, 131:1825-1834.
doi: 10.1007/s00122-018-3115-5 |
[11] |
Peng Z S, Li X, Yang Z J, Liao M L. A new reduced height gene found in the tetraploid semi-dwarf wheat landrace Aiganfanmai. Gen Mol Res, 2011, 10:2349-2357.
doi: 10.4238/2011.October.5.5 |
[12] | Yang T Z, Zhang X K, Liu H W, Wang Z H. Rht21 in common wheat variety—XN0004 Rht21 in common wheat variety—XN0004. Acta Univ Agric Boreali-Occident, 1993, 21:13-17. |
[13] |
Wu J, Kong X, Wan J, Liu X, Zhang X, Guo X, Zhou R, Zhao G, Jing R, Fu X, Jia J. Dominant and pleiotropic effects of a GAI gene in wheat results from a lack of interaction between DELLA and GID1. Plant Physiol, 2011, 157:2120-2130.
doi: 10.1104/pp.111.185272 pmid: 22010107 |
[14] |
Pearce S, Saville R, Vaughan S P, Chandler P M, Wilhelm E P, Sparks C A, Al-Kaff N, Korolev A, Boulton M I, Phillips A L, Hedden P, Nicholson P, Thomas S G. Rht-1 dwarfing genes in hexaploid wheat Rht-1 dwarfing genes in hexaploid wheat. Plant Physiol, 2011, 157:1820-1831.
doi: 10.1104/pp.111.183657 |
[15] |
Wu Q, Chen Y, Xie J, Dong L, Wang Z, Lu P, Wang R, Yuan C, Zhang Y, Liu Z. A 36 Mb terminal deletion of chromosome 2BL is responsible for a wheat semi-dwarf mutation. Crop J, 2020, 9:873-881.
doi: 10.1016/j.cj.2020.06.015 |
[16] |
Ellis M H, Rebetzke G J, Azanza F, Richards R A, Spielmeyer W. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theor Appl Genet, 2005, 111:423-430.
pmid: 15968526 |
[17] |
Bazhenov M S, Divashuk M G, Amagai Y, Watanabe N, Karlov G I. Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker. Mol Breed, 2015, 35:213.
doi: 10.1007/s11032-015-0407-1 |
[18] |
Daba S D, Tyagi P, Brown-Guedira G, Mohammadi M. Genome-wide association study in historical and contemporary U.S. winter wheats identifies height-reducing loci. Crop J, 2020, 8:243-251.
doi: 10.1016/j.cj.2019.09.005 |
[19] | Gale M D, Youssefian S. Dwarfing Genes in Wheat. England: Plant Breeding Institute, 1985. |
[20] |
Sun L, Yang W, Li Y, Shan Q, Ye X, Wang D, Yu K, Lu W, Xin P, Pei Z, Guo X, Liu D, Sun J, Zhan K, Chu J, Zhang A. Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line Rht12, which reduces stem cell length and affects gibberellic acid synthesis, is a 5AL terminal deletion line. Plant J, 2019, 97:887-900.
doi: 10.1111/tpj.2019.97.issue-5 |
[21] |
Wang Y, Du Y, Yang Z, Chen L, Condon A G, Hu Y G. Rht13 and Rht8 on plant height and some agronomic traits in common wheat Rht13 and Rht8 on plant height and some agronomic traits in common wheat. Field Crops Res, 2015, 179:35-43.
doi: 10.1016/j.fcr.2015.04.010 |
[22] |
Vikhe P, Venkatesan S, Chavan A, Tamhankar S, Patil R. Rht14 in durum wheat and its effect on seedling vigor, internode length and plant height Rht14 in durum wheat and its effect on seedling vigor, internode length and plant height. Crop J, 2019, 7:187-197.
doi: 10.1016/j.cj.2018.11.004 |
[23] |
Ford B A, Foo E, Sharwood R, Karafiatova M, Vrana J, Macmillan C, Nichols D S, Steuernagel B, Uauy C, Dolezel J, Chandler P M, Spielmeyer W. Rht18 semi-dwarfism in wheat is due to increased GA 2-oxidaseA9 expression and reduced GA content. Plant Physiol, 2018, 177:168-180.
doi: 10.1104/pp.18.00023 |
[24] |
Mo Y, Vanzetti L S, Hale I, Spagnolo E J, Guidobaldi F, Al-Oboudi J, Odle N, Pearce S, Helguera M, Dubcovsky J. Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development Rht25, a locus on chromosome arm 6AS affecting wheat plant height, heading time, and spike development. Theor Appl Genet, 2018, 131:2021-2035.
doi: 10.1007/s00122-018-3130-6 |
[25] |
Chen S, Gao R, Wang H, Wen M, Xiao J, Bian N, Zhang R, Hu W, Cheng S, Bie T, Wang X. Rht23) regulating panicle morphology and plant architecture in bread wheat Rht23) regulating panicle morphology and plant architecture in bread wheat. Euphytica, 2014, 203:583-594.
doi: 10.1007/s10681-014-1275-1 |
[26] |
Würschum T, Langer S M, Longin C F H, Tucker M R, Leiser W L. A modern green revolution gene for reduced height in wheat. Plant J, 2017, 92:892-903.
doi: 10.1111/tpj.13726 |
[27] |
Wang M, Wang S, Xia G. From genome to gene: a new epoch for wheat research? Trends Plant Sci, 2015, 20:380-387.
doi: 10.1016/j.tplants.2015.03.010 |
[28] | 陈昊, 谭晓风. 基于第二代测序技术的基因资源挖掘. 植物生理学报, 2014, 50:1089-1095. |
Chen H, Tan X F. Excavation of genic resources based on next generation sequencing technologies. Acta Phytophysiol Sin, 2014, 50:1089-1095 (in Chinese with English abstract). | |
[29] |
Winfield M O, Wilkinson P A, Allen A M, Barker G L, Coghill J A, Burridge A, Hall A, Brenchley R C, D'amore R, Hall N, Bevan M W, Richmond T, Gerhardt D J, Jeddeloh J A, Edwards K J. Targeted re-sequencing of the allohexaploid wheat exome. Plant Biotechnol J, 2012, 10:733-742.
doi: 10.1111/j.1467-7652.2012.00713.x pmid: 22703335 |
[30] |
Paux E, Roger D, Badaeva E, Gay G, Bernard M, Sourdille P, Feuillet C. Characterizing the composition and evolution of homoeologous genomes in hexaploid wheat through BAC-end sequencing on chromosome 3B. Plant J, 2006, 48:463-474.
doi: 10.1111/tpj.2006.48.issue-3 |
[31] |
Jordan K W, Wang S, Lun Y, Gardiner L J, Maclachlan R, Hucl P, Wiebe K, Wong D, Forrest K L, Consortium I, Sharpe A G, Sidebottom C H, Hall N, Toomajian C, Close T, Dubcovsky J, Akhunova A, Talbert L, Bansal U K, Bariana H S, Hayden M J, Pozniak C, Jeddeloh J A, Hall A, Akhunov E. A haplotype map of allohexaploid wheat reveals distinct patterns of selection on homoeologous genomes. Genome Biol, 2015, 16:1-18.
doi: 10.1186/s13059-014-0572-2 |
[32] | 许达兴. 小麦茎秆快速发育基因qd1的遗传定位与转录组学分析. 中国农业科学院硕士学位论文, 北京, 2018. |
Xu D X. Genetic Mapping of the qd1 Gene of Wheat Stem Quick Development and Transcriptome Analysis in Wheat. MS Thesis of Chinese Academy of Agricultural Sciences, Beijing, China, 2018 (in Chinese with English abstract). | |
[33] |
Robert K, Nicholas B, Ricardo R G, Coghill J A, Archana P, Keywan H P, Cristobal U, Phillips A L. Mutation scanning in wheat by exon capture and next-generation sequencing. PLoS One, 2015, 10:e0137549.
doi: 10.1371/journal.pone.0137549 |
[34] |
Hill J T, Demarest B L, Bisgrove B W, Gorsi B, Su Y C, Yost H J. MMAPPR: mutation mapping analysis pipeline for pooled RNA-seq. Genome Res, 2013, 23:687-697.
doi: 10.1101/gr.146936.112 |
[35] | Lincoln S E, Daly M J, Lander E. Constructing Genetic Linkage Maps with MAPMAKER/EXP version 3.0: a Tutorial and Reference Manual, Technical Report, 3rd edn. USA: Whitehead Institute for Biomedical Research, 1993. |
[36] |
Xiong H C, Li Y T, Guo H J, Xie Y D, Zhao L S, Gu J Y, Zhao S R, Ding Y P, Liu L X. Genetic mapping by integration of 55K SNP array and KASP markers reveals candidate genes for important agronomic traits in hexaploid wheat. Front Plant Sci, 2021, 12:628478.
doi: 10.3389/fpls.2021.628478 |
[37] | 张在宝, 李婉杰, 李九丽, 张弛, 胡梦辉, 程琳, 袁红雨. 植物RNA结合蛋白研究进展. 中国农业科学, 2018, 15:4007-4019. |
Zhang Z B, Li W J, Li J L, Zhang C, Hu M H, Cheng L, Yuan H Y. The research progress of plant RNA binding proteins. Sci Agric Sin, 2018, 15:4007-4019 (in Chinese with English abstract). | |
[38] | 王天一, 王应祥, 尤辰江. 植物PHD结构域蛋白的结构与功能特性. 遗传, 2021, 43:323-339. |
Wang T Y, Wang Y X, You C J. Structural and functional characteristics of plant PHD domain-containing proteins. Hereditas, 2021, 43:323-339 (in Chinese with English abstract). | |
[39] |
Hofmann K. A superfamily of membrane-bound O-acyltransferases with implications for Wnt signaling. Trends Biochem Sci, 2000, 25:111-112.
pmid: 10694878 |
[40] | 马小凤, 刘子金, 郑超星, 王星, 武宇, 李洪杰, 张根发. 植物烯醇化酶基因ENO2的功能研究进展. 植物遗传资源学报, 2018, 19:1030-1037. |
Ma X F, Liu Z J, Zheng C X, Wang X, Wu Y, Li H J, Zhang G F. Status and progress on functions of plant enolase gene ENO2. J Plant Genet Resour, 2018, 19:1030-1037 (in Chinese with English abstract). | |
[41] | 钟明志, 魏淑红, 彭正松, 杨在君. 小麦Rht矮秆基因研究和应用综述. 分子植物育种, 2018, 16:6670-6677. |
Zhong M Z, Wei S H, Peng Z S, Yang Z J. A review of the research and application of Rht dwarf genes in wheat. Mol Plant Breed, 2018, 16:6670-6677 (in Chinese with English abstract). | |
[42] | Worland A J, Sayers E J, Korzun V. Rht8 locus and its significance in international breeding programs Rht8 locus and its significance in international breeding programs. Euphytica, 2001, 119:155-159. |
[43] |
Kowalski A M, Gooding M, Ferrante A, Slafer G A, Orford S, Gasperini D, Griffiths S. Rht8 in contrasting nitrogen treatments and water regimes Rht8 in contrasting nitrogen treatments and water regimes. Field Crops Res, 2016, 191:150-160.
doi: 10.1016/j.fcr.2016.02.026 |
[44] |
Tian X, Wen W, Xie L, Fu L, Xu D, Fu C, Wang D, Chen X, Xia X, Chen Q, He Z, Cao S. Rht24 in bread wheat Rht24 in bread wheat. Front Plant Sci, 2017, 8:1379.
doi: 10.3389/fpls.2017.01379 |
[45] |
Korzun V, Roder M S, Ganal M W, Worland A J, Law C N. Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I: Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat(Triticum aestivum L.). Theor Appl Genet, 1998, 96:1104-1109.
doi: 10.1007/s001220050845 |
[46] |
Chai L, Chen Z, Bian R, Zhai H, Cheng X, Peng H, Yao Y, Hu Z, Xin M, Guo W, Sun Q, Zhao A, Ni Z. Triticum aestivum L.) Triticum aestivum L.). Theor Appl Genet, 2019, 132:1815-1831.
doi: 10.1007/s00122-019-03318-z |
[47] |
Miura K, Rus A, Sharkhuu A, Yokoi S, Karthikeyan A S, Raghothama K G, Baek D, Koo Y D, Jin J B, Bressan R A, Yun D J, Hasegawa P M. Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses Arabidopsis SUMO E3 ligase SIZ1 controls phosphate deficiency responses. Proc Natl Acad Sci USA, 2005, 102:7760-7765.
doi: 10.1073/pnas.0500778102 |
[48] | 唐娜, 姜莹, 何蓓如, 胡银岗. 赤霉素敏感性不同矮秆基因对小麦胚芽鞘长度和株高的效应. 中国农业科学, 2009, 42:3774-3784. |
Tang N, Jiang Y, He B R, Hu Y G. Effects of dwarfing genes of Rht-B1b, Rht-D1b and Rht8 with different response to GA3 on coleoptile length and plant height of wheat. Sci Agric Sin, 2009, 42:3774-3784 (in Chinese with English abstract). | |
[49] |
Hedden P, Sponsel V. A century of gibberellin research. J Plant Growth Regul, 2015, 34:740-760.
doi: 10.1007/s00344-015-9546-1 |
[50] |
Gasperini D, Greenland A, Hedden P, Dreos R, Harwood W, Griffiths S. Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids. J Exp Bot, 2012, 63:4419-4436.
doi: 10.1093/jxb/ers138 pmid: 22791821 |
[1] | 张一铎, 李国强, 孔忠新, 王玉泉, 李小利, 茹振钢, 贾海燕, 马正强. 基因聚合选育抗赤霉病小麦新品系百农4299[J]. 作物学报, 2022, 48(9): 2221-2227. |
[2] | 谭照国, 苑少华, 李艳梅, 白建芳, 岳洁茹, 刘子涵, 张天豹, 赵福永, 赵昌平, 许本波, 张胜全, 庞斌双, 张立平. 小麦TaPIP1基因克隆及其在花药开裂中潜在功能分析[J]. 作物学报, 2022, 48(9): 2242-2254. |
[3] | 冯子恒, 李晓, 段剑钊, 高飞, 贺利, 杨天聪, 戎亚思, 宋莉, 尹飞, 冯伟. 基于特征波段选择和机器学习的小麦白粉病高光谱遥感监测[J]. 作物学报, 2022, 48(9): 2300-2314. |
[4] | 曹际玲, 曾青, 朱建国. 不同品种小麦灌浆期旗叶光合特性及光合基因表达对臭氧浓度升高的响应[J]. 作物学报, 2022, 48(9): 2339-2350. |
[5] | 李永波, 崔德周, 黄琛, 隋新霞, 樊庆琦, 楚秀生. 高度特异性小麦ATG8抗体的研制及其在细胞自噬检测中的应用[J]. 作物学报, 2022, 48(9): 2390-2399. |
[6] | 王云奇, 高福莉, 李傲, 郭同济, 戚留冉, 曾寰宇, 赵建云, 王笑鸽, 高国英, 杨佳鹏, 白金泽, 马亚欢, 梁月馨, 张睿. 小麦花后穗部温度变化规律及其与产量的关系[J]. 作物学报, 2022, 48(9): 2400-2408. |
[7] | 惠志明, 徐建飞, 简银巧, 卞春松, 段绍光, 胡军, 李广存, 金黎平. 基于2b-RAD测序的四倍体马铃薯熟性相关的分子标记开发[J]. 作物学报, 2022, 48(9): 2274-2284. |
[8] | 委刚, 陈单阳, 任德勇, 杨宏霞, 伍靖雯, 冯萍, 王楠. 水稻细长秆突变体sr10的鉴定与基因定位[J]. 作物学报, 2022, 48(8): 2125-2133. |
[9] | 徐云碧, 王冰冰, 张健, 张嘉楠, 李建生. 应用分子标记技术改进作物品种保护和监管[J]. 作物学报, 2022, 48(8): 1853-1870. |
[10] | 王沙沙, 黄超, 汪庆昌, 晁岳恩, 陈锋, 孙建国, 宋晓. 小麦籽粒大小相关基因TaGS2克隆及功能分析[J]. 作物学报, 2022, 48(8): 1926-1937. |
[11] | 杜启迪, 郭会君, 熊宏春, 谢永盾, 赵林姝, 古佳玉, 赵世荣, 丁玉萍, 宋希云, 刘录祥. 小麦顶端小穗退化突变体asd1基因定位[J]. 作物学报, 2022, 48(8): 1905-1913. |
[12] | 冯亚娟, 李廷轩, 蒲勇, 张锡洲. 不同镉积累类型小麦各器官镉积累分布规律及机理分析[J]. 作物学报, 2022, 48(7): 1761-1770. |
[13] | 刘阿康, 马瑞琦, 王德梅, 王艳杰, 杨玉双, 赵广才, 常旭虹. 覆膜和补施氮肥对晚播冬小麦冬前植株生长及群体质量的影响[J]. 作物学报, 2022, 48(7): 1771-1786. |
[14] | 王娟, 刘翼, 姚丹妤, 邹景伟, 肖世和, 孙果忠. 小麦生殖发育阶段对低温的敏感性鉴定[J]. 作物学报, 2022, 48(7): 1721-1729. |
[15] | 张少华, 段剑钊, 贺利, 井宇航, 郭天财, 王永华, 冯伟. 基于无人机平台多模态数据融合的小麦产量估算研究[J]. 作物学报, 2022, 48(7): 1746-1760. |
|