Ceratotropis亚属基于SSR标记和ITS序列的分类与进化分析

doi:10.3724/SP.J.1006.2010.01585

摘要/Abstract

摘要： 利用SSR分子标记和ITS序列分析研究Ceratotropis亚属已鉴定的93份种质以及Vigna亚属的2份地方种和Glycine属的1份野生种，共8个种及亚种的96份材料的遗传差异。初步筛选出分布于11个连锁群(linkage group, LG)的74对小豆引物及14对黒吉豆引物和第9连锁群的5对绿豆引物，得到分辨力强的27对引物，平均PIC值为0.4914；UPGMA聚类分析，在DICE系数0.021处，将95份参试材料(除HAI_103)划分为两大类，第I类包括Ceratotropis组所有种质和3个“过渡”种质，第II类包括Angulares组所有种质；且小豆引物通用性最高：23对小豆引物的转移能力达88%以上。对参试材料中的28份典型种质进行ITS测序分析，比较序列间Kimura进化距离发现，V. minima和 V. umbellata种间距离最小(0~0.005)，V. radiata种内分化程度最高；利用MEGA4.0构建最大简约树(maximum parsimony, MP)和邻接树(neighbor-joining, NJ)，将参试种质划分为3大类，这与形态学和SSR标记分类一致。

关键词: Ceratotropis亚属, 分类, SSR标记, ITS序列, 进化

Abstract: Ninety-three materials in subgenus Ceratotropis, two landraces ofsubgenus Vigna and one accession of Glycine soja from various regions of China were analyzed by using simple sequence repeat (SSR) makers and sequencing nrDNA internal transcribed spacer (ITS) for the genetic evaluation. Twenty-sevenspecific SSR primers were selected by screening 74 azuki bean primers and 14 blackgram primers that distributed in 11 linkage groups and five mungbean primers in linkage group 9 with the average polymorphic information content (PIC) value 0.4914. In UPGMA cluster analysis, 95 materials excluding HAI_103 (G. soja) were divided into Group I (including section Ceratotropis and three “transitional” materials) and Group II (section Angulares). And the transferability of 23 primers from azuki bean reached 88%. As for ITS analysis, Kimura-2-parameter distance was calculated based on ITS sequences of 28 representing materials. The shortest distance was found between V. minima and V. umbellata with 0–0.005 and V. radiata was the most intra-specific divergent. Phylogenetic trees constructed by maximum parsimony (MP) and neighbor-joining (NJ) methods with MEGA 4.0 showed that 28 materials were divided into three groups. This result was consistent with the classification results by phenotypes and SSR makers. Therefore, it is anticipated that more information for classification of subgenus Ceratotropis can be provided by these two molecular methods and also a system for evaluation combined by morphological characteristics can be established.

Key words: Subgenus Ceratotropis, Classification,SSR markers, ITS sequences, Evolution

马燕玲,程须珍,王丽侠,王素华,赵丹. Ceratotropis亚属基于SSR标记和ITS序列的分类与进化分析[J]. 作物学报, 2010, 36(09): 1585-1595.

MA Yan-Ling, CHENG Xu-Zhen, WANG Li-Xia, WANG Su-Hua, ZHAO Dan. Classification and Evolution Analysis for Vigna Subgenus Ceratotropis Based on SSR Markers and ITS Sequences[J]. Acta Agron Sin, 2010, 36(09): 1585-1595.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2010.01585

https://zwxb.chinacrops.org/CN/Y2010/V36/I09/1585

参考文献

[1] Marechal R, Mascherpa J M, Stainier F. Etude taxonomique dun groupe complexe despeces des genres Phaseolus et Vigna (Pap ilionaceae) sur la base de donneesmorphologiques et polliniques, traitees par lanalyse informatique. Boissiera, 1978, 28: 1-237
[2] Baudoin J P, Marechal R. Taxonomy and evolution of the genus Vigna. In: Shanmugasundaram S, McLean B T, eds. Proceedings of the Second International Mungbean Symposium. Asian Vegetable Research and Development Center, Shanhua, Tainan, 1988. pp 2-12
[3] Tomooka N, Vaughan D A, Moss H. The Asian Vigna: Genus Vigna Subgenus Ceratotropis Genetic Resources. London: Kluwer Academic Publishers, 2002. pp 92-184
[4] Tomooka N, Egawa Y, Kaga A. Biosystematics and genetic resources of the genus Vigna subgenus Ceratotropis. In: 7thMAFF International Workshop on Genetic Resources, Wild Legumes. Tsukuba, Japan, 2000. pp 37-62
[5] Dong Y-C(董玉琛), Liu X(刘旭). Chinese Crops and Wild Relatives: Volume of Crops (中国作物及其野生近缘植物: 粮食作物卷). Beijing: China Agriculture Press, 2007. pp 412-426 (in Chinese)
[6] Tomooka N, Kaga A, Isemura T, Vaughan. Vigna genetic resources. In: 14th MAFF International Workshop on Genetic Resources. Tsukuba, Japan, 2009
[7] Maekawa F. Topo-morphological and taxonomical studies in Phaseoleae, Leguminosae. Jpn J Bot, 1955, 15: 103-116
[8] Egawa Y, Nakagahra M, Fernandez G C J. Cytogenetical analysis of tetraploid Vigna glabrescens by interspecific hybridization involving diploid Asian Vigna species. In: Proceedings of the Second International Mungbean Symposium, 1988. pp 200-204
[9] Chen N C, Baker L R, Honma S. Interspecific crossability among four species of Vigna food legumes
[J].Euphytica
[10] Tomooka N, Yoon M S, Doi K, Kaga A, Vaughan D. AFLP analysis of diploid species in the genus Vigna subgenus Ceratotropis
[J].Genet Resour Crop Evol
[11] Doi K, Kaga A, Tomooka N, Vaughan D. Molecular phylogeny of genus Vigna subgenus Ceratotropis based on rDNA ITS and atpB-rbcL intergenic spacer of cpDNA sequences
[J].Genetica
[12] Dixit A, Ma K H, Yu J W, Cho E G, ParkY J. Reverse transcriptase domain sequences from mungbean (Vigna radiata) LTR retrotransposons: sequence characterization and phylogenetic analysis
[J].Plant Cell Rep
[13] Saini A, Jawali N. Molecular evolution of 5S rDNA region in Vigna subgenus Ceratotropis and its phylogenetic implications. Plant Syst Evol, 2009, 280: 187–206
[14] He H-H(贺浩华), Luo X-J(罗小金), Zhu C-L(朱昌兰), He X-P(贺晓鹏), Fu J-R(傅军如), Sun J-L(孙俊立), Zhang H-L(张洪亮), Li Z-C(李自超). Classification for some sterile lines and their restorers of hybrid rice with SSR Markers. Acta Agron Sin (作物学报), 2006, 32(2): 169-175 (in Chinese with English abstract)
[15] Wu X-L(吴晓雷), He C-Y(贺超英), Chen S-Y(陈受宜). Genetic diversity and evolution relationships of genus Glycine. Prog Nat Sci (自然科学进展), 2001, 11(7): 689-698 (in Chinese)
[16] Dikshit H K, Jhang T, Singh N K. Genetic differentiation of Vigna species by RAPD, URP and SSR markers
[J].Biol Plant
[17] Li X-Y(李学营), Peng J-Y(彭建营), Bai R-X(白瑞霞). Use of ITS sequence on nuclear rDNA for phylogenetic research of seed plants. Acta Bot Boreali-Occident Sin 西北植物学报), 2005, 25(4): 829-834 (in Chinese with English abstract)(
[18] Goel S, Raina S N, Ogihara Y. Molecular evolution and Phylogenetic implications of internal transcribed spacer sequences of nuclear ribosomal DNA in the Phaseolus-Vigna complex. Mol Phylogenet Evol, 2002, 22: 12-19
[19] Liang H-W(梁宏伟), Wang C-Z(王长忠), Li Z(李忠), Luo X-Z(罗相忠), Zou G-W(邹桂伟). Improvement of the silver-stained technique of polyacrylamide gel Electrophoresis. Hereditas (遗传), 2008, 30(10): 1379-1382 (in Chinese with English abstract)
[20] Saini A, Reddy S K, Jawali N. Intra-individual and intra-species heterogeneity in nuclear rDNA ITS region of Vigna species from subgenus Ceratotropis
[J]. Genet Res Camb
[21] Wang L-X(王丽侠), Cheng X-Z(程须珍), Wang S-H(王素华), Liu C-Y(刘长友), Liang H(梁辉). Transferability of SSR from adzuki bean to mungbean. Acta Agron Sin (作物学报), 2009, 35(5): 816−820 (in Chinese with English abstract)
[22] Hou X(侯鑫), Liu J-E(刘俊娥), Zhao Y-Z(赵一之), Zhao L-Q(赵利清). Interspecific relationships of Caragana microphylla, C. davazamcii and C. korshinskii (Leguminosae) based on ITS and trnL-F data sets. Acta Phytotaxonomica Sin (植物分类学报), 2006, 44(2): 126-134 (in Chinese with English abstract)
[23] Vir R, Bhat K V, Lakhanpau S. Transferability of sequences tagged microsatellite sites (STMS) primers to pulse yielding taxa belonging to Phaseolae. Intl J Interg Biol, 2009, 5: 62-66
[24] Gopinathan M C, Babu C R. Structural diversity and its adaptive significance in seeds of Vigna minima (Roxb.) Ohwi & Ohashi and its allies (Leguminosae-Papilionoideae).Ann Bot,1985, 56: 723-732
[25] Lin R-F(林汝法), Chai Y(柴岩), Liao M(廖敏), Sun S-X(孙世贤). Minor Grain Crops in China. Beijing: China Agricultural and Technical Press, 2002. pp 193-208 (in Chinese)
[26] Tateishi Y. A Revision of the Azuki Bean Group, the Subgenus Ceratotropis of the Genus Vigna (Leguminosae). PhD Dissertation of Tohoku University, 1985 (in Japanese)
[27] Wang Z-C(王子成), Li Z-A(李忠爱), Li S-P(李锁平). The technology of MSAP and its applications in plant. Biotechnol Bull (生物技术通报), 2006, (suppl): 195-196 (in Chinese with English abstract)
[28] Yang X-Y(杨秀英), Gao Y(高英), Shen M(沈敏). Comparative analysis of chromosomal karyotype for three species of Vigna savi
[J]. Plant J Tianjin Norm Univ (Nat Sci Edn) (天津师范大学学报·自然科学版.2003, 23(4):23-26
[29] Stebbins G L. Chromosomal Evolution in Higher Plants. London: Edward Arnold Ltd, 1971. pp 85-104

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