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作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1766-1774.doi: 10.3724/SP.J.1006.2012.01766

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

水稻DUS测试标准品种丛矮2号矮化多分蘖表型的遗传基础

王涛1,袁守江2,尹亮2,赵金凤1,万建民1,3,李学勇1,*   

  1. 1中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程, 北京 100081; 2山东省水稻研究所, 山东济南 250100; 3 南京农业大学作物遗传与种质创新国家重点实验室 / 江苏省植物基因工程技术研究中心, 江苏南京 210095
  • 收稿日期:2012-03-01 修回日期:2012-06-10 出版日期:2012-10-12 网络出版日期:2012-07-27
  • 通讯作者: 李学勇, E-mail: xueyong.li@caas.net.cn; Tel: 010-82109716
  • 基金资助:

    本研究由国家转基因生物新品种培育科技重大专项(2011ZX08009-003)和留学人员科技活动择优资助项目“农作物分枝机理及应用研究”资助。

Genetic Basis of the High-Tillering Dwarf Trait in the Rice DUS Test Standard Variety Cong’ai 2

WANG Tao1,YUAN Shou-Jiang2,YIN Liang2,ZHAO Jin-Feng1,WAN Jian-Min1,3,LI Xue-Yong1,*   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Shandong Rice Research Institute, Jinan 250100, China; 3 National Key Laboratory for Crop Genetics and Germplasm Enhancement, Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-03-01 Revised:2012-06-10 Published:2012-10-12 Published online:2012-07-27
  • Contact: 李学勇, E-mail: xueyong.li@caas.net.cn; Tel: 010-82109716

摘要:

水稻DUS测试标准品种之一丛矮2号(cl2)具有矮化多分蘖的表型特征,遗传分析表明该性状由1对隐性核基因控制,已将其定位在第4染色体长臂InDel标记C4-CL5和C4-CL4之间。对这两个标记之间一个已报道的多分蘖基因D17/HTD1进行测序,发现cl2中的D17/HTD1基因编码区第1 796个碱基由C突变为T,从而导致第599位的氨基酸由脯氨酸变成亮氨酸。同时对另一个来源于粳稻品种日本晴的矮化多分蘖突变体S1-40进行测序,发现D17/HTD1第3内含子3’端拼接点由AG突变为AA,导致mRNA产生2种错误的剪接形式。D17/HTD1编码类胡萝卜素裂解双加氧酶7 (Carotenoid Cleavage Dioxygenase7, CCD7),参与新型植物激素独脚金内酯(Strigolactones, SLs)的合成。利用SLs的人工合成类似物GR24处理cl2,其多分蘖表型得到抑制。系统进化树分析发现CCD7在几乎所有植物都有同源蛋白,水稻CCD7蛋白与同属禾本科的玉米、高粱和短柄草同源性最高。Real-time RT-PCR结果显示D17/HTD1基因在植物所有组织都有表达,尤以茎部最高。

关键词: 丛矮2号, 矮化多分蘖, 独脚金内酯, 类胡萝卜素裂解双加氧酶

Abstract:

Cong’ai 2 (cl2), one of the standard varieties of rice DUS test in China, shows typical high-tillering dwarf phenotype.Genetic analysis showed that this trait is controlled by a single recessive nucleus gene, which was mapped between two InDel markers C4-CL5 and C4-CL4 on the long arm of chromosome 4. Between these two markers, there is a known gene D17/HTD1, mutations in which caused high-tillering dwarf phenotype. Sequencing analysis of the D17/HTD1 allele in cl2 revealed that the 1796th base was substituted from C to T, changing the 599th amino acid from proline to leucine. Another high-tillering dwarf mutant S1-40 was obtained from japonica variety Nipponbare mutagenized by EMS. The 3' splicing site of the 3rd intron of D17/HTD1 was substituted from AG to AA,which caused the formation of two aberrant transcripts in S1-40. D17/HTD1 encodes the carotenoid cleavage dioxygenase 7 (CCD7), which is a key enzyme involved in the biosynthesis of a new plant hormone strigolactones (SLs). Exogenous application of GR24, a synthetic analogue of SLs, inhibited the tillering phenotype of cl2. Phylogenetic analysis revealed that CCD7 homologs existed in almost all plant species. Real-time RT-PCR showed D17/HTD1 was expressed in all rice tissues examined, with the highest expression in stems.

Key words: Cong&rsquo, ai 2, High-tillering dwarf, Strigolactones, Carotenoid-cleavage dioxygenase

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