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作物学报

作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1775-1781.doi: 10.3724/SP.J.1006.2012.01775

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

以标记辅助选择改良江苏主栽粳稻品种“淮稻5号”黑条矮缩病抗性

李爱宏1,*，潘存红1,**，戴正元1，肖宁1，余玲1，李育红1，张小祥1，张洪熙1，潘学彪2   

  1. 1 江苏里下河地区农业科学研究所 / 国家水稻改良中心南京分中心扬州试验站，江苏扬州225007; 2 扬州大学植物功能基因组学教育部重点实验室，江苏扬州225009
  • 收稿日期:2012-02-13 修回日期:2012-06-20 出版日期:2012-10-12 网络出版日期:2012-07-27
  • 通讯作者: 李爱宏, E-mail: yzlah@126.com
  • 基金资助:

    本研究由国家自然科学基金项目(30900886), 江苏省自然科学基金项目(BK2011428), 江苏省农业科技自主创新资金项目(CX(11)1022, 4001), 江苏省“333工程”科研资助项目(BRA2011080)和江苏省“六大人才高峰”项目和扬州市科技攻关项目(yz2009029, yz2010042)资助。

Using Marker-Assisted Selection to Improve the Resistance to Rice Black-Streaked Dwarf Viral Disease of Huaidao 5, an Elite Japonica Rice Cultivar in Jiangsu

LI Ai-Hong1,*,PAN Cun-Hong1,**,DAI Zheng-Yuan1,XIAO Ning1,YU Ling1,LI Yu-Hong1,ZHANG Xiao-Xiang1,ZHANG Hong-Xi1,PAN Xue-Biao2   

  1. 1 Lixiahe Agricultural Research Institute of Jiangsu Province/Yangzhou Station of Nanjing Sub-center of National Rice Improvement Center, Yangzhou 225007, China; 2 Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2012-02-13 Revised:2012-06-20 Published:2012-10-12 Published online:2012-07-27
  • Contact: 李爱宏, E-mail: yzlah@126.com

PDF

1020

被引次数

9

摘要：

水稻黑条矮缩病是由灰飞虱传播的一种病毒病，最近在江苏、浙江等省暴发给水稻生产造成严重威胁。本研究在前期对水稻黑条矮缩病抗性基因初步定位的基础上，针对第6染色体短臂上紧密连锁的两个抗性QTL，以抗性亲本“明恢63”为供体，感病亲本“淮稻5号”为轮回亲本，通过标记辅助选择，构建了携带目标抗性QTL的系列近等基因系。自然接种和人工接种鉴定表明新培育的近等基因系其发病率较对照轮回亲本下降25%左右，抗性得到显著改良。农艺性状调查结果显示大多数近等基因系其生育期、株高及产量构成性状等与轮回亲本相似，表明目标区段存在较少的累赘连锁。此外，对有关如何实现目标QTL的精细定位，以及利用标记辅助选择聚合多个抗性QTL培育抗病品种的策略也进行了探讨。

关键词: 水稻黑条矮缩病, 淮稻5号, 标记辅助选择, 近等基因系, 抗性评价

Abstract:

Rice black-streaked dwarf viral disease (RBSDV), a kind of viral disease transmitted by small plant hopper, broke out recently in Jiangsu and Zhejiang provinces of China and posed a serious threat to rice production. Based on mapping of the QTLsfor RBSDV resistance previously, we used the resistant parent Minghui 63 as donor, the susceptible japonica cultivar Huaidao 5 as recurrent parent, and bred a series of Near-isogenic lines (NILs) aiming at two closely linked QTLs on the short arm of Chromosome 6 in rice. Natural inoculation and artificial inoculation results showed that RBSDV incidence rate of NILs was about 25% lower than that of recurrent parent and the resistance of NILs was improved significantly. Agronomic traits of most NILs, such as heading date, plant height, and yield components were similar to those of recurrent parent, indicating that the target chromosome region contains few linkage drags. The strategies for fine mapping the target QTLs and pyramiding more QTLs in the way of breed improvement were also discussed.

Key words: RBSDV, Huaidao 5, MAS, NILs, Resistance evaluation

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