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作物学报 ›› 2012, Vol. 38 ›› Issue (10): 1775-1781.doi: 10.3724/SP.J.1006.2012.01775

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

以标记辅助选择改良江苏主栽粳稻品种“淮稻5号”黑条矮缩病抗性

李爱宏1,*，潘存红1,**，戴正元1，肖宁1，余玲1，李育红1，张小祥1，张洪熙1，潘学彪2   

  1. 1 江苏里下河地区农业科学研究所 / 国家水稻改良中心南京分中心扬州试验站，江苏扬州225007; 2 扬州大学植物功能基因组学教育部重点实验室，江苏扬州225009
  • 收稿日期:2012-02-13 修回日期:2012-06-20 出版日期:2012-10-12 网络出版日期:2012-07-27
  • 通讯作者: 李爱宏, E-mail: yzlah@126.com
  • 基金资助:

    本研究由国家自然科学基金项目(30900886), 江苏省自然科学基金项目(BK2011428), 江苏省农业科技自主创新资金项目(CX(11)1022, 4001), 江苏省“333工程”科研资助项目(BRA2011080)和江苏省“六大人才高峰”项目和扬州市科技攻关项目(yz2009029, yz2010042)资助。

Using Marker-Assisted Selection to Improve the Resistance to Rice Black-Streaked Dwarf Viral Disease of Huaidao 5, an Elite Japonica Rice Cultivar in Jiangsu

LI Ai-Hong1,*,PAN Cun-Hong1,**,DAI Zheng-Yuan1,XIAO Ning1,YU Ling1,LI Yu-Hong1,ZHANG Xiao-Xiang1,ZHANG Hong-Xi1,PAN Xue-Biao2   

  1. 1 Lixiahe Agricultural Research Institute of Jiangsu Province/Yangzhou Station of Nanjing Sub-center of National Rice Improvement Center, Yangzhou 225007, China; 2 Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China
  • Received:2012-02-13 Revised:2012-06-20 Published:2012-10-12 Published online:2012-07-27
  • Contact: 李爱宏, E-mail: yzlah@126.com

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1019

被引次数

9

摘要：

水稻黑条矮缩病是由灰飞虱传播的一种病毒病，最近在江苏、浙江等省暴发给水稻生产造成严重威胁。本研究在前期对水稻黑条矮缩病抗性基因初步定位的基础上，针对第6染色体短臂上紧密连锁的两个抗性QTL，以抗性亲本“明恢63”为供体，感病亲本“淮稻5号”为轮回亲本，通过标记辅助选择，构建了携带目标抗性QTL的系列近等基因系。自然接种和人工接种鉴定表明新培育的近等基因系其发病率较对照轮回亲本下降25%左右，抗性得到显著改良。农艺性状调查结果显示大多数近等基因系其生育期、株高及产量构成性状等与轮回亲本相似，表明目标区段存在较少的累赘连锁。此外，对有关如何实现目标QTL的精细定位，以及利用标记辅助选择聚合多个抗性QTL培育抗病品种的策略也进行了探讨。

关键词: 水稻黑条矮缩病, 淮稻5号, 标记辅助选择, 近等基因系, 抗性评价

Abstract:

Rice black-streaked dwarf viral disease (RBSDV), a kind of viral disease transmitted by small plant hopper, broke out recently in Jiangsu and Zhejiang provinces of China and posed a serious threat to rice production. Based on mapping of the QTLsfor RBSDV resistance previously, we used the resistant parent Minghui 63 as donor, the susceptible japonica cultivar Huaidao 5 as recurrent parent, and bred a series of Near-isogenic lines (NILs) aiming at two closely linked QTLs on the short arm of Chromosome 6 in rice. Natural inoculation and artificial inoculation results showed that RBSDV incidence rate of NILs was about 25% lower than that of recurrent parent and the resistance of NILs was improved significantly. Agronomic traits of most NILs, such as heading date, plant height, and yield components were similar to those of recurrent parent, indicating that the target chromosome region contains few linkage drags. The strategies for fine mapping the target QTLs and pyramiding more QTLs in the way of breed improvement were also discussed.

Key words: RBSDV, Huaidao 5, MAS, NILs, Resistance evaluation

[1]Milne R G, Lovisolo O. Maize rough dwarf and related viruses. Adv Virus Res, 1977, 21: 267–341



[2]Azuhata F, Uyeda I, Kimura I, Shikata E. Close similarity between genome structures of rice black-streaked dwarf and maize rough dwarf viruses. J Gen Virol, 1993, 74: 1227–1232



[3]Wang H D, Chen J P, Wang A G, Jiang X H, Adams M J. Studies on the epidemiology and yield losses from rice black-streaked dwarf disease in a recent epidemic in Zhejiang province, China. Plant Pathol, 2009, 58: 815-825



[4]Chen S-X(陈声祥), Wu H-L(吴惠玲), Liao X-G(廖璇刚), Lü Y-P(吕永平), Shen S-F(沈升法), Wang O-F(王藕芳), Jin M-S(金梅松). The prevalent reason of rice black-streaked dwarf viral disease in the middle of Zhejiang. Zhejiang Agric Sci (浙江农业科学), 2000, (6): 287–289 (in Chinese)



[5]Qiu X-P(仇学平), Cheng L-P(程来品), Yang J(杨军), Zhang L-C(张连成), Hu Y(胡毓). Investigation of susceptibility of different rice varieties to rice black-streaked dwarf virus disease. Shanghai Agric Sci (上海农业科技), 2010, (4): 131 (in Chinese)



[6]Ren S-M(任世民), Hu W-Y(胡务义). The prevalent characteristics and control techniques of rice black-streaked dwarf viral diseases in Chunan. Hangzhou Agric Sci (杭州农业科技), 2007, (4): 7–9 (in Chinese)



[7]Li A-H(李爱宏), Dai Z-Y(戴正元), Ji H-J(季红娟), Zhang X-X(张小祥), Li Y-H(李育红), Pan C-H(潘存红), Zhang H-X(张洪熙), Pan X-B(潘学彪). Preliminary analysis on resistance of rice black-streaked dwarf viral disease for germplasms with different gene-types. J Yangzhou Univ (Agric & Life Sci Edn)(扬州大学学报•农业与生命科学版), 2008, 29(3): 18–22 (in Chinese with English abstract)



[8]Wang B-X(王宝祥), Jiang L(江玲), Chen L-M(陈亮明), Lu B-G(卢百关), Wang Q(王琦), Li G-Q(黎光泉), Fan J-W(樊继伟), Cheng X-N(程遐年), Zhai H-Q(翟虎渠), Xu D-Y(徐大勇), Wan J-M(万建民). Screeing of rice resources against rice black-streaked dwarf virus and mapping of resistant QTL. Acta Agron Sin (作物学报), 2010, 36(8): 1258–1264 (in Chinese with English abstract) 



[9]Pan C-H(潘存红), Li A-H(李爱宏), Chen Z-X(陈宗祥), Wu L-B(吴林波), Dai Z-Y(戴正元), Zhang H-X(张洪熙), Huang N-S(黄年生), Chen X-J(陈夕军), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Pan X-B(潘学彪). Detection of QTL for resistance to rice black-streaked dwarf viral disease. Acta Agron Sin (作物学报), 2009, 35(12): 2213–2217 (in Chinese with English abstract)



[10]Hospital F, Chevalet C, Mulsant P. Using markers in gene introgression breeding programs. Genetics, 1992, 132: 1199–1210



[11]Lu Y-J(卢扬江), Zheng K-L(郑康乐). A simple method for extracting rice DNA. Chin J Rice Sci(中国水稻科学), 1992, 6(1): 47–48 (in Chinese with English abstract)



[12]Lü Y-P(吕永平), Lei J-L(雷娟利), Jin D-D(金登迪), Chen S-X(陈声祥). Detect RBSDV with RT-PCR. Acta Agric Zhejiangensis (浙江农业学报), 2002, 14(2): 117–119 (in Chinese)



[13]Wu A-G(吴爱国), Zhang S-C(张守成), Gu K-L(顾开联), Zhang R-Q(张如群), Ma L(马林). Screening for resistant rice varieties in Lixiahe region. Chin Agric Tech Exten (中国农技推广), 2006, (4): 18–19 (in Chinese)



[14]Li A-H(李爱宏), Dai Z-Y(戴正元), Ji H-J(季红娟), Zhang X-X(张小祥), Li Y-H(李育红), Pan C-H(潘存红), Zhang H-X(张洪熙), Pan X-B(潘学彪). Analysis on the combining ability of resistance to rice black-streaked dwarf viral disease (RBSDV) in indica hybrid rice. J Yangzhou Univ (Agric & Life Sci Edn)(扬州大学学报•农业与生命科学版), 2009, 30(1): 18–22 (in Chinese with English abstract)



[15]Chen S-X(陈声祥), Yu J-B(余舰斌), Qin W-S(秦文胜), Liu L(刘力), Gao D-M(高东明), Xu J(徐静), Wang Y-F(王一风). Studies on stunt disease of hybrid rice in Wenzhou. Virol Sin (中国病毒学), 1993, 8(4): 373–378 (in Chinese with English abstract)



[16]Wang H-D(王华弟), Zhu Z-R(祝增荣), Chen J-P(陈剑平), Wang E-G(汪恩国), Li B-F(李宝福). Epidemics, monitoring and key control techniques of the rice black-streaked dwarf viral disease. Acta Agric Zhejiangensis (浙江农业学报), 2007, 19(3): 141–146 (in Chinese with English abstract)



[17]Pan X-B(潘学彪), Zhang Y-F(张亚芳), Zuo S-M(左示敏), Chen Z-X(陈宗祥). Discussion on QTLs identification and application for important quantitative traits in crops. J Yangzhou Univ (Agric & Life Sci Edn)(扬州大学学报•农业与生命科学版), 2005, 26(2): 50–55 (in Chinese with English abstract)



[18]Luo L-G(罗林广), Xu J-F(徐俊峰), Zhai H-Q(翟虎渠), Wan J-M(万建民). Analysis of photoperiod-sensitivity genes in Minghui 63, a restorer line of indica rice (Oryza sativa L.). Acta Genet Sin (遗传学报), 2003, 30(9): 804–810 (in Chinese with English abstract)



[19]Wei X J, Jiang L, Xu J F, Liu X, Liu S J, Zhai H Q, Wan J M. The distribution of japonica rice cultivars in the lower region of the Yangtze River valley is determined by its photoperiod-sensitivity and heading date genotypes. J Integr Plant Biol, 2009, 51: 922–932



[20]Yano M, Katayose Y, Ashikari M, Yamanouchi U, Monna L, Fuse T, Baba T,Yamanoto K, Umehara Y, Nagamura Y, and Sasaki T. Hd1, a major photoperiod sensitivity quantitative trait locus in rice, is closely related to the Arabidopsis flowering time gene CONSTANS. Plant Cell, 12: 2473–2483
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