大豆叶柄角的QTL定位分析

doi:10.3724/SP.J.1006.2020.94056

摘要/Abstract

摘要：

叶柄角是大豆株型的重要构成因素, 影响大豆冠层结构、光合作用效率以及最终产量。解析大豆叶柄角的遗传基础对提升大豆产量具有重要意义。本研究以2个叶柄角具有显著差异的亲本BLA和SLA以及它们衍生的RIL群体为材料, 构建高密度的遗传图谱, 对大豆不同部位的叶柄角进行QTL分析, 并利用近等基因系验证部分QTL。遗传分析结果显示, 叶柄角呈连续正态分布, 符合数量性状遗传特征。利用GBS技术构建了包含859个Bin标记的大豆高密度遗传图谱, 总遗传长度为2326.9 cM, 标记间平均距离为2.763 cM; 共检测到14个调控叶柄角的QTL, LOD值在2.58~4.80之间, 可解释遗传变异范围在6.9%~12.4%之间, 其中5个QTL定位在第12染色体上且成簇存在; 构建的qLA12和qLA18的近等基因系表型结果显示, 叶柄角在同一对近等基因家系间差异显著, 表明qLA12和qLA18是2个可信的QTL。本研究为进一步克隆调控叶柄角功能基因奠定了基础, 为大豆理想株型育种提供了遗传材料。

关键词: 大豆株型, 叶柄角, QTL定位, 近等基因系

Abstract:

Leafstalk angle is one of the most important elements for shoot architecture of soybean, affecting canopy architecture, photosynthetic efficiency and final grain yield. Exploring genetic basis of soybean leafstalk angle is significant to improve soybean yield. In this study, two soybean accessions BLA and SLA, contrasting in leafstalk angle, and their derived RIL population were used for high resolution genetic map construction and QTL detection for leafstalk angle, further the near-isogenic lines (NIL) were constructed to validate partial QTLs. Genetic analysis results showed that values of leafstalk angle performed serial and normal distribution which coincides with genetic characteristics of quantitative traits. Additionally, a high resolution genetic map consisting of 859 bin markers was constructed by using GBS method. The linkage map covered 2326.9 cM of genetic distance and the average distance between two markers was 2.763 cM. A total of 14 QTLs for regulating leafstalk angle were detected, with explained 6.9%-12.4% of genetic variation, and their LOD values varied from 2.58 to 4.80, and five of them were clustered together on Chromosome 12. The phenotype of the NILs for qLA12 and qLA18 revealed that leafstalk angle performed significant difference between same pair of NILs which strongly suggested that qLA12 and qLA18 are two believable QTLs. In summary, our results lay a foundation for cloning functional genes of regulating leafstalk angle and provide genetic resources for breeding elite soybean varieties with ideal shoot architecture.

Key words: soybean shoot architecture, leafstalk angle, QTL mapping, near-isogenic lines (NIL)

王存虎,刘东,许锐能,杨永庆,廖红. 大豆叶柄角的QTL定位分析[J]. 作物学报, 2020, 46(01): 9-19.

WANG Cun-Hu,LIU Dong,XU Rui-Neng,YANG Yong-Qing,LIAO Hong. Mapping of QTLs for leafstalk angle in soybean[J]. Acta Agronomica Sinica, 2020, 46(01): 9-19.

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链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2020.94056

https://zwxb.chinacrops.org/CN/Y2020/V46/I01/9

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性状 Trait	亲本Parents		重组自交系群体RILs							遗传力 h²_b
性状 Trait	BLA±SE	SLA±SE	均值 Mean	标准差SD	变异系数 CV (%)	最小值Min.	最大值Max.	峰度 Skew.	偏度 Kurt.	遗传力 h²_b
LA-H	36.60±1.36	36.60±2.72	37.25	6.57	17.64	20	75	0.78	2.29	0.70
LA-M	53.30±1.36	51.67±3.60	56.61	8.82	15.58	30	85	0.56	0.52	0.72
LA-L	95.00±2.36	73.30±2.72	74.83	12.17	16.26	20	110	-1.22	3.16	0.90

染色体 Chr.	遗传距离 Genetic distance (cM)	物理长度 Physical length	标记数量 Number of bin markers	平均覆盖距离 Average coverage distance (cM)
Chr01	132.25	56,510,449	45	2.94
Chr02	149.48	47,706,575	39	3.83
Chr03	82.60	45,730,193	38	2.17
Chr04	125.97	51,405,255	50	2.52
Chr05	99.11	41,919,006	34	2.91
Chr06	128.98	50,892,625	44	2.93
Chr07	113.88	43,534,570	44	2.59
Chr08	166.04	47,709,553	45	3.69
Chr09	157.61	50,173,153	32	4.93
Chr10	85.61	51,542,279	25	3.42
Chr11	158.21	34,671,664	53	2.99
Chr12	123.75	40,030,102	47	2.63
Chr13	135.19	45,514,498	45	3.00
Chr14	87.35	47,841,279	44	1.99
Chr15	106.51	51,710,377	48	2.22
Chr16	74.30	37,192,783	46	1.62
Chr17	90.05	41,348,852	45	2.00
Chr18	113.71	57,961,359	46	2.47
Chr19	103.79	50,522,110	46	2.26
Chr20	92.53	47,868,276	43	2.15
合计Total	2326.90	941,784,958	859	55.26

性状 Trait	QTL	染色体 Chr.	标记区间 Markers interval	遗传位置 Genetic position (cM)	LOD	加性效应Add	表型变异PVE (%)
上位 LA-H	qLA12a	Gm12	Chr12.3021337-Chr12.4783251	20.466	3.55	1.76	9.3
	qLA12b	Gm12	Chr12.6175142-Chr12.6947560	32.213	3.43	1.61	9.0
	qLA12c	Gm12	Chr12.2086945-Chr12.2529118	7.231	2.70	1.46	7.2
	qLA01	Gm01	Chr01.55523848-Chr01.56419281	131.054	3.85	-1.67	10.1
	qLA11	Gm11	Chr11.10213098-Chr11.24373759	57.808	2.58	1.55	6.9
中位LA-M	qLA09	Gm09	Chr09.4074635-Chr09.4606081	34.147	3.97	-2.36	10.4
	qLA12d	Gm12	Chr12.1868502-Chr12.2529118	6.231	3.86	2.30	10.1
	qLA12a	Gm12	Chr12.3021337-Chr12.4783251	17.466	3.19	2.33	8.4
	qLA14	Gm14	Chr14.6976251-Chr14.43836321	60.580	2.70	-2.70	7.2
	qLA17	Gm17	Chr17.37570037-Chr17.36696957	49.792	2.62	-1.87	7.0
下位LA-L	qLA18	Gm18	Chr18.46032220-Chr18.47375074	63.343	4.80	3.98	12.4
	qLA06	Gm06	Chr06.11495976-Chr06.15321801	70.098	4.21	-4.69	11.0
	qLA15	Gm15	Chr15.11748320-Chr15.47611763	83.523	2.94	-3.20	7.8
	qLA07	Gm07	Chr07.7016819-Chr07.8103486	29.912	2.71	3.02	7.2

引物编号 Primer name	SNP位点 SNP loci	内切酶 Enzyme	引物序列 Primer sequence (5°-3°)	预期片段大小 Expected product size (bp)
dCAPS-Chr12-1	3465298	Spe I	F: AGTGCTAAAAACAATCCCCG	250/(226+24)
dCAPS-Chr12-1	3465298	Spe I	R: GAGCCCGAATGAAGTGGGTGAGGACTAG	250/(226+24)
dCAPS-Chr12-2	3513670	Bsm I	F: GCATAGCCTCTCCAATCCAT	229/(198+31)
dCAPS-Chr12-2	3513670	Bsm I	R: GCATTTGAATGAAGACTTGTGGAGGAATG	229/(198+31)
dCAPS-Chr12-3	3577958	Bmr I	F: CTGAGGATGTGATCCTGAGAAATGCTACTG	194/(161+33)
dCAPS-Chr12-3	3577958	Bmr I	R: CTCCACCCATCATCAAAATA	194/(161+33)
dCAPS-Chr12-4	3593743	Acl I	F: TGCCAACATTCCCTCATCAG	186/(160+26)
dCAPS-Chr12-4	3593743	Acl I	R: TTAGATGAAGCAAATGATTCAAATAACGT	186/(160+26)
dCAPS-Chr12-5	3953314	SnaB I	F: CTTAAACCACCCGGTGTCTCTTGCGGTACG	234/(205+29)
dCAPS-Chr12-5	3953314	SnaB I	R: GTGTGTTACTATCTCTCTCT	234/(205+29)
dCAPS-Chr12-6	4183623	Bsm I	F: GGAAACTTGGTTAATAGCTAAGATCCTTGA	215/(183+32)
dCAPS-Chr12-6	4183623	Bsm I	R: GATTGGAGGAAAGTGGAAGA	215/(183+32)
dCAPS-Chr12-7	4546007	Sma I	F: ACTCCCTTCTTGTTGCCTTG	169/(141+28)
dCAPS-Chr12-7	4546007	Sma I	R: AATTGCTTGATAAGGTCCTGCTAGGCCCG	169/(141+28)
dCAPS-Chr18-1	45231954	Taq I	F: CTCTCATCTCAAACAAGTCT	209/(181+28)
dCAPS-Chr18-1	45231954	Taq I	R: GAGTTAATCCTAGTTTAAAATGTCATTTCG	209/(181+28)
dCAPS-Chr18-2	46986384	Sna I	F: GAAGTAACAACAGACTACGACGGTTGTAT	230/(205+25)
dCAPS-Chr18-2	46986384	Sna I	R: CTAAGACGGACCTACAAAGC	230/(205+25)
dCAPS-Chr18-3	47034338	Asu I	F: GTAGACACCGGCGATCGGGGACTTTGGTC	204/(181+23)
dCAPS-Chr18-3	47034338	Asu I	R: CAGCAAGTCCACCAGCATCT	204/(181+23)
引物编号 Primer name	SNP位点 SNP loci	内切酶 Enzyme	引物序列 Primer sequence (5°-3°)	预期片段大小 Expected product size (bp)
dCAPS-Chr18-4	48080921	Taq II	F: GTCGTGAAATTTGAGCACCACGTTCGCCA	247/(221+26)
			R: TATCTCGCAAATCCCAACGT
dCAPS-Chr18-5	48514645	Mnl I	F: TGGTTGTTCTGTTTTTCTTTGTTCTACCT	244/(215+29)
dCAPS-Chr18-5	48514645	Mnl I	R: GTCGACTTCTATCTACAAAC	244/(215+29)
dCAPS-Chr18-6	50618122	Xmn I	F: CTATACTCGGTCACTTATTG	195/(167+28)
dCAPS-Chr18-6	50618122	Xmn I	R: CTTCTTAGTAACCCTTTCCAAGTGAAAACA	195/(167+28)
dCAPS-Chr18-7	51595218	Dpn I	F: GGTCACGTTGTTTTGTAAGAATGTTCCGA	242/(213+29)
dCAPS-Chr18-7	51595218	Dpn I	R: AACCACAAGCGTAATGAGAT	242/(213+29)