燕麦SNP高密度遗传图谱构建及β-葡聚糖含量QTL定位

doi:10.3724/SP.J.1006.2024.31060

摘要/Abstract

摘要：

β-葡聚糖是燕麦发挥保健作用的主要功能因子，提高其含量对优质燕麦生产有着重要意义。为促进高β-葡聚糖燕麦种质资源的有效利用和相关基因发掘，本研究以高β-葡聚糖品种夏莜麦和低β-葡聚糖品种赤38组配衍生的219个家系RIL8群体为材料，利用重测序技术构建了包含21个连锁群，5032个bin标记的遗传连锁图谱，图谱总长2045.09 cM，平均图距0.42 cM。利用标准酶法和近红外法对4个环境的RIL群体家系β-葡聚糖含量进行测定，结合测定结果，利用完备区间作图法对β-葡聚糖含量进行QTL定位分析，结果显示不同环境条件下RIL群体β-葡聚糖含量呈正态分布，并出现超亲后代家系，4个环境下群体β-葡聚糖含量变异系数介于9.06%~16.63%之间。QTL定位检测到7个与燕麦β-葡聚糖含量相关的QTL，分布于2D、3D、4C和4D染色体上，其中贡献率最高为14.73%，在2个环境中检测到同一个QTL，其标记区间为Chr4C_mark8361257-Chr4C_mark8384831。研究结果将为燕麦β-葡聚糖分子标记辅助育种提供重要的理论依据。

关键词: 燕麦, SNP, 遗传图谱, β-葡聚糖, QTL定位

Abstract:

β-glucan is the main functional component of oats for health care, and improving its content is of great significance to the production of high-quality oats. In this study, in order to promote the effective utilization of high β-glucan oat germplasm resources and the discovery of related genes, a genetic linkage map containing 21 linkage groups and 5032 bin markers was constructed by using resequencing technology with the 223 RIL8 populations derived from the high β-glucan variety Xiayoumai and the low β-glucan resource Chi38. The total length of the map was 2045.09 cM, and the average plot distance was 0.42 cM. The β-glucan content of RIL populations in 4 environments was determined by standard enzyme method and near infrared method. Combined with the determination results, QTL analysis of β-glucan content was performed by complete interval mapping method. The results showed that the β-glucan content of RIL populations was normally distributed under different environmental conditions, and there were superparent descendants. The coefficient of variation of β-glucan content in the four environments ranged from 9.06% to 16.63%. Seven QTLs related to β-glucan content in oat were detected by QTL mapping, distributed on 2D, 3D, 4C, and 4D chromosomes, with the highest contribution rate of 14.73%. The same QTL was detected in two environments with a marker interval of Chr4C_mark8361257–Chr4C_mark8384831. The results of this study provide an important theoretical basis for molecular marker-assisted breeding of oat β-glucan.

Key words: oat, SNP, genetic map, β-glucan, QTL mapping

韩丽, 汤胜胜, 李佳, 胡海斌, 刘龙龙, 吴斌. 燕麦SNP高密度遗传图谱构建及β-葡聚糖含量QTL定位[J]. 作物学报, 0, (): 0-.

HAN Li, TANG Sheng-Sheng, LI Jia, HU Hai-Bin, LIU Long-Long, WU Bin. Construction of SNP high-density genetic map and localization of QTL for β-glucan content in oats[J]. Acta Agronomica Sinica, 0, (): 0-.

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