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玉米果穗相关性状QTL定位及重要候选基因分析

郑雪晴1，王兴荣2，张彦军2，龚佃明1,*，邱法展1,*   

  1. 1华中农业大学作物遗传改良全国重点实验室 / 湖北洪山实验室，湖北武汉430070; 2甘肃省农业科学院作物研究所, 甘肃兰州730070
  • 收稿日期:2023-10-25 修回日期:2024-01-12 接受日期:2024-01-12 网络出版日期:2024-02-19
  • 基金资助:
    本研究由科技兴蒙行动重点专项项目(2022EEDSKJXM011, 2022EEDSKJXM011-1)和山东省重点研发计划项目(2022CXPT014)资助。

Mapping of QTLs for ear-related traits and prediction of key candidate genes in maize

ZHENG Xue-Qing1,WANG Xing-Rong2,ZHANG Yan-Jun2,GONG Dian-Ming1,*,QIU Fa-Zhan1,*   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Hubei Hongshan Laboratory, Wuhan, 430070, China; 2 Gansu Academy of Agricultural Sciences, Lanzhou 730070, Gansu, China
  • Received:2023-10-25 Revised:2024-01-12 Accepted:2024-01-12 Published online:2024-02-19
  • Supported by:
    This study was supported by the Key Program of Action Plan to Revitalize Inner Mongolia through Science and Technology (2022EEDSKJXM011, 2022EEDSKJXM011-1) and the National Key Research and Development Program of Shandong Province (2022CXPT014).

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摘要：

玉米果穗相关性状与产量直接相关，其遗传基础解析对于指导玉米遗传改良意义重大。本研究对3年6个环境下的168份高代回交重组自交系(AB-RILs)的穗长、穗行数和百粒重等8个性状进行表型鉴定，结合玉米10 K芯片产生的覆盖全基因组的11,407个SNP(Single Nucleotide Polymorphisms)标记对8个性状进行QTL定位。共鉴定到32个与8个果穗性状相关的QTL，其中包含5个环境一致性的QTL，3个多效性QTL。进一步利用507份关联群体的基因型与表型数据对主效QTL候选区间进行关联分析，鉴定到19个可能与果穗性状相关的重要候选基因，结合候选基因的进化分析和表达分析等，初步确定其中4个为关键候选基因。以上结果为玉米育种中果穗性状的遗传改良提供了重要的标记信息，同时也为果穗性状相关基因克隆提供指导。

关键词: 玉米, AB-RIL群体, 果穗, 籽粒, QTL定位, 候选区间关联分析

Abstract:

Maize ear related traits are directly related to yield, and the analysis of their genetic basis is of great significance for guiding maize genetic improvement. In this study, the phenotypic characteristics of eight traits were identified in 168 high generation backcross recombinant inbred lines (AB-RILs) in six environments over three years. QTLs for eight traits were mapped with 11,407 SNP markers generated by 10 K liquid chip in maize. A total of 32 QTL related to eight ear traits were identified in this study, including five environmentally consistent QTLs and three pleiotropic QTL. Further, we used the genotypic and phenotypic data of 507 maize inbred lines to analyze the candidate regions of major QTL and identified 19 candidate genes that might be related to ear shape. We finally speculated four genes as candidate genes based on the analysis of evolution and expression of the genes. These results provide the important marker information for the genetic improvement of ear traits in maize breeding and offered guidance for the cloning of genes related to ear traits. 

Key words: maize, AB-RIL population, ear, kernel, QTL mapping, candidate regional association analysis

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