欢迎访问作物学报，今天是
作物学报

作物学报

• •    

两个RIL群体中小麦籽粒品质相关性状QTL定位及KASP标记开发

毕俊鸽1,2,**，曾占奎1,2,**，李琼1,2，洪壮壮1,2，颜群翔1,2，赵越1,2，王春平1, 2,*   

  1. 1河南科技大学农学院 / 河南省旱地作物种质资源利用工程研究中心, 河南洛阳 471000; 2神农种业实验室, 河南郑州 450000
  • 收稿日期:2023-11-30 修回日期:2024-01-31 接受日期:2024-01-31 网络出版日期:2024-03-02
  • 通讯作者: 毕俊鸽, E-mail: bijungee@163.com; 曾占奎, E-mail: zengzhankui0301@163.com
  • 基金资助:
    本研究由河南省重大科技专项“小麦营养基因组学解析及功能食品创制与产业化”项目(231100110300)和神农种业实验室“一流课题”项目(SN01-2022-01)资助。

This study was supported by the Major Science and Technology of Henan Province Project “The Wheat Nutrigenomics Analysis and Functional Food Creation and Industrialization Fund Project” (231100110300), and the Shennong Laboratory “first-class subject” project (SN01-2022-01).

QTL mapping and KASP marker development of grain quality-relating traits in two wheat RIL populations   

  1. 1 College of Agriculture, Henan University of Science and Technology / Henan Dryland Crop Germplasm Resources Utilization Engineering Research Center, Luoyang 471000, Henan, China; 2Shennong Laboratory, Zhengzhou 45000, Henan, China
  • Received:2023-11-30 Revised:2024-01-31 Accepted:2024-01-31 Published online:2024-03-02
  • Supported by:

    This study was supported by the Major Science and Technology of Henan Province Project “The Wheat Nutrigenomics Analysis and Functional Food Creation and Industrialization Fund Project” (231100110300), and the Shennong Laboratory “first-class subject” project (SN01-2022-01).

PDF

12

摘要：

本研究利用小麦55K SNP (55K single-nucleotide polymorphism)芯片和DArT (diversity array technology)标记对Avocet/Chilero和Avocet/Huites构建的两个F6重组自交系群体(recombinant inbred line, RIL)进行了小麦籽粒蛋白质含量(grain protein content, GPC)、湿面筋含量(wet gluten content, WGC)和沉降值(sedimentation value, SV)的QTL(quantitative trait loci)定位。共鉴定到68个与小麦籽粒蛋白质含量、湿面筋含量和沉降值相关的QTL，表型贡献率为3.60%~22.53%，其中，位于3A(2)、4D、5D(2)、6A(8)和7B染色体上的14个QTL可在多环境下被重复检测到。此外，在3A、3D、4B、5D、6A(2)和7B染色体上检测到7个QTL簇，位于3AS染色体9.32~60.01 Mb和6AS染色体38.47~82.95 Mb的稳定QTL簇C3A和C6A.2，同时与小麦籽粒蛋白质含量、湿面筋含量和沉降值显著相关，分别解释了6.55%~14.21%和3.83%~22.53%的表型变异。同时，在两个QTL簇中筛选到16个可能与籽粒蛋白质含量相关的候选基因，并根据候选基因开发了可供育种利用的KASP标记CGPC-6A-KASP-1和CGPC-6A-KASP-2。本研究为小麦籽粒品质相关性状的遗传改良提供了新的QTL位点和功能标记，为分子标记辅助育种提供依据与参考。

关键词: 小麦, 籽粒蛋白质含量, QTL定位, KASP标记开发

Abstract:

We utilized a 55K single-nucleotide polymorphism (55K SNP) array and diversity array technology (DArT) to identify QTLs for grain protein content (GPC), wet gluten content (WGC), and sedimentation value (SV) in two F6 recombinant inbred lines derived from Avocet/Chilero and Avocet/Huites. A total of QTL was identified related to grain protein content, wet gluten content, and settlement value, explaining 3.60%–22.53% of the phenotypic variances. 14 QTL were found to be present in multiple environments, located on chromosomes 3A(2), 4D, 5D(2), 6A(8), and 7B, respectively. Additionally, seven QTL clusters were detected on chromosomes 3A, 3D, 4B, 5D, 6A(2), and 7B, respectively. Two stable QTL clusters, C3A and C6A.2, identified in the physical intervals of 9.32–60.01 Mb and 38.47–82.95 Mb, respectively, were significantly associated with grain protein content and wet gluten content. These clusters accounted for 6.55%–14.21% and 3.83%–22.53% of the phenotypic variances for grain protein content, wet gluten content, and sedimentation value. Meanwhile, a total of 16 candidate genes associated with grain protein content were predicted within the two stable QTL clusters. Moreover, two KASP marker, CGPC-6A-KASP-1 and CGPC-6A-KASP-2, were developed based on the candidate genes. The findings of this study provide support for the identification of new QTL and functional markers that can contribute to the genetic improvement of grain quality-related traits in wheat. These results also offer valuable insights for marker-assisted breeding in wheat.

Key words: wheat, grain protein content, QTL mapping, KASP tag development

[1] 张振, 赵俊晔, 石玉, 张永丽, 于振文. 不同播幅对小麦花后叶片光合特性和产量的影响[J]. 作物学报, 2024, 50(4): 981-990.
[2] 许乃银, 金石桥, 晋芳, 刘丽华, 徐剑文, 刘丰泽, 任雪贞, 孙全, 许栩, 庞斌双. 基于SNP标记的小麦品种遗传相似度及其检测准确度分析[J]. 作物学报, 2024, 50(4): 887-896.
[3] 黄宏胜, 张馨月, 居辉, 韩雪. 大气CO2浓度升高背景下冬小麦冠层光谱特征和地上生物量估算[J]. 作物学报, 2024, 50(4): 991-1003.
[4] 王添宁, 冯雅岚, 琚吉浩, 吴毅, 张均, 马超. 小麦及其祖先物种GRF转录因子家族鉴定与表达分析[J]. 作物学报, 2024, 50(4): 897-813.
[5] 齐学礼, 李莹, 李春盈, 韩留鹏, 赵明忠, 张建周. 基于转录组探究外源水杨酸对条锈菌侵染小麦幼苗的缓解效应及差异表达基因分析[J]. 作物学报, 2024, 50(4): 1080-1090.
[6] 琚吉浩, 马超, 王添宁, 吴毅, 董钟, 方美娥, 陈钰姝, 张均, 付国占. 小麦TaPOD家族的全基因组鉴定及表达分析[J]. 作物学报, 2024, 50(3): 779-792.
[7] 张宝华, 刘佳静, 田晓, 田旭钊, 董阔, 武郁洁, 肖凯, 李小娟. 小麦TaSPX1基因的克隆、表达及耐低氮逆境的功能研究[J]. 作物学报, 2024, 50(3): 576-589.
[8] 张月, 王志慧, 淮东欣, 刘念, 姜慧芳, 廖伯寿, 雷永. 花生含油量的遗传基础与QTL定位研究进展[J]. 作物学报, 2024, 50(3): 529-542.
[9] 郝倩琳, 杨廷志, 吕新茹, 秦慧敏, 王亚林, 贾晨飞, 夏先春, 马武军, 徐登安. 小麦胚芽鞘长度QTL定位和GWAS分析[J]. 作物学报, 2024, 50(3): 590-602.
[10] 赵荣荣, 丛楠, 赵闯. 基于Landsat 8影像提取豫中地区冬小麦和夏玉米分布信息的最佳时相选择[J]. 作物学报, 2024, 50(3): 721-733.
[11] 范子培, 李龙, 史雨刚, 孙黛珍, 李超男, 景蕊莲. 小麦TabHLH112-2B基因克隆及每穗小穗数相关功能标记开发[J]. 作物学报, 2024, 50(2): 403-413.
[12] 张康, 聂志刚, 王钧, 李广. 温度升高下APSIM模型春小麦籽粒生长参数敏感性分析及优化[J]. 作物学报, 2024, 50(2): 464-477.
[13] 谭丹, 陈家婷, 郜钰, 张晓军, 李欣, 闫贵云, 李锐, 陈芳, 常利芳, 张树伟, 郭慧娟, 畅志坚, 乔麟轶. 小麦穗型相关生长素通路基因发掘及TaARF23-A与小穗数关联分析[J]. 作物学报, 2024, 50(2): 506-513.
[14] 李艳, 方宇辉, 王永霞, 彭超军, 华夏, 齐学礼, 胡琳, 许为钢. 不同磷胁迫处理转OsPHR2小麦的转录组学分析[J]. 作物学报, 2024, 50(2): 340-353.
[15] 谢炜, 贺鹏, 马宏亮, 雷芳, 黄秀兰, 樊高琼, 杨洪坤. 秋闲期秸秆覆盖与施磷对冬小麦氮素吸收利用的影响[J]. 作物学报, 2024, 50(2): 440-450.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2