芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析

doi:10.3724/SP.J.1006.2025.44175

作物学报 ›› 2025, Vol. 51 ›› Issue (5): 1166-1177.doi: 10.3724/SP.J.1006.2025.44175

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析

张金泽¹，周庆国²，肖莉晶¹，金海润¹，欧阳青静¹，龙旭¹，晏中彬¹，田恩堂^1,*

1贵州大学农学院, 贵州贵阳550025; 2安徽鼎科种业有限责任公司, 安徽合肥230001

收稿日期:2024-10-20 修回日期:2025-01-23 接受日期:2025-01-23 出版日期:2025-05-12 网络出版日期:2025-02-17
基金资助:
本研究由贵州省科技支撑计划项目(黔科合支撑[2022]重点031), 国家自然科学基金项目(32160483, 32360497)和贵州大学国家自然科学基金后补助项目([2023]093号)。

QTL mapping and candidate gene analysis of glucosinolate content in various tissues of Brassica juncea

ZHANG Jin-Ze¹,ZHOU Qing-Guo²,XIAO Li-Jing¹,JIN Hai-Run¹,OU-YANG Qing-Jing¹,LONG Xu¹,YAN Zhong-Bin¹,TIAN En-Tang^1,*

1 College of Agriculture, Guizhou University, Guiyang 550025, Guizhou, China; 2Anhui Dingke Seed Industry Co, Ltd., Hefei 230001, Anhui, China

Received:2024-10-20 Revised:2025-01-23 Accepted:2025-01-23 Published:2025-05-12 Published online:2025-02-17
Supported by:
This study was supported by the Guizhou Provincial Science and Technology Support Program (Guizhou Kehe Support [2022] Key 031), the National Natural Science Foundation of China (32160483, 32360497), and the Post-Funded Project for National Natural Science Foundation of Guizhou University ([2023] No. 093）.

摘要/Abstract

摘要：

硫苷是重要的次生代谢物，在油菜生长发育、病虫害防御等方面都具有重要作用。本研究以芥菜型油菜品系YufengZC和Qichi881为亲本创建的包含197个RIL-F₈株系的作图群体(QY-RIL群体)为研究材料，测定了全部株系的叶片、茎秆、花蕾和种子的硫苷含量。结果表明，同一株系不同组织间硫苷含量存在显著差异，而不同株系间相同组织的硫苷含量也存在较大变异，且总体呈正态分布。相关性分析发现，花蕾、茎秆与种子间硫苷含量呈显著正相关关系，而叶片的硫苷含量仅与花蕾呈显著正相关关系。此外，本研究还对叶片、茎秆、花蕾和种子硫苷含量的调控基因进行了QTL定位，分别鉴定到9、10、9和18个QTL，包含6个在多组织中共同检测到的cQTL (consensus QTL)。结合cQTL区间序列信息及所含基因的表达分析结果，初步筛选出6个候选基因，其中BjuB018426 (GTR1)和BjuB020498 (GTR2)参与硫苷从营养组织向生殖组织的转运，可能是造成本研究中硫苷含量差异分布的重要调控基因。本研究结果可为解析芥菜型油菜不同组织的硫苷合成与分配机制及选育不同硫苷含量的多功能品种奠定基础。

关键词: 芥菜型油菜, 硫苷含量, QTL定位, 候选基因

Abstract:

Glucosinolates are critical secondary metabolites that play a pivotal role in the growth and development of rapeseed, as well as in its defense against diseases and pests. In this study, a mapping population comprising 197 recombinant inbred lines (RILs) of Brassica juncea was utilized to investigate glucosinolate distribution and genetic regulation. The glucosinolate contents in leaves, stems, buds, and seeds were quantified across all lines. The results revealed significant differences in glucosinolate content among different tissues within the same line and considerable variation across lines within the species, exhibiting an overall normal distribution. Correlation analysis showed a strong positive correlation in glucosinolate content among buds, stems, and seeds, as well as a notable positive correlation between leaves and flower buds. To further elucidate the genetic regulation of glucosinolate content, quantitative trait locus (QTL) mapping was conducted, resulting in the identification of 9, 10, 9, and 18 QTLs associated with glucosinolate content in leaves, stems, buds, and seeds, respectively. By integrating QTL interval sequence information with gene expression data, six candidate genes were preliminarily identified. Among them, BjuB018426 (GTR1) and BjuB020498 (GTR2) were implicated in the transport of glucosinolates from vegetative tissues to reproductive tissues, suggesting their potential roles as key regulatory genes underlying the differential distribution of glucosinolates observed in this study. These findings provide valuable insights into the mechanisms governing glucosinolate synthesis and distribution across various tissues in Brassica juncea. Moreover, they offer a foundational basis for breeding multifunctional varieties with tailored glucosinolate profiles to meet diverse agricultural and industrial needs.

Key words: Brassica juncea, glucosinolate contents, QTL mapping, candidate genes

张金泽, 周庆国, 肖莉晶, 金海润, 欧阳青静, 龙旭, 晏中彬, 田恩堂. 芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析[J]. 作物学报, 2025, 51(5): 1166-1177.

ZHANG Jin-Ze, ZHOU Qing-Guo, XIAO Li-Jing, JIN Hai-Run, OU-YANG Qing-Jing, LONG Xu, YAN Zhong-Bin, TIAN En-Tang. QTL mapping and candidate gene analysis of glucosinolate content in various tissues of Brassica juncea[J]. Acta Agronomica Sinica, 2025, 51(5): 1166-1177.

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芥菜型油菜不同组织硫苷含量的QTL定位与候选基因分析

QTL mapping and candidate gene analysis of glucosinolate content in various tissues of Brassica juncea

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