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作物学报 ›› 2021, Vol. 47 ›› Issue (11): 2080-2090.doi: 10.3724/SP.J.1006.2021.01089

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系

黄义文1(), 代旭冉1, 刘宏伟1, 杨丽1, 买春艳2, 于立强3, 于广军3, 张宏军1,*(), 李洪杰1,*(), 周阳1,*()   

  1. 1中国农业科学院作物科学研究所 / 作物分子育种国家工程实验室, 北京 100081
    2新乡县矮败小麦育种技术创新中心, 河南新乡 453731
    3石家庄市农林科学研究院赵县实验基地, 河北赵县 051530
  • 收稿日期:2020-11-20 接受日期:2021-03-19 出版日期:2021-11-12 网络出版日期:2021-04-01
  • 通讯作者: 张宏军,李洪杰,周阳
  • 作者简介:E-mail: 18838916683@163.com
  • 基金资助:
    河北省现代种业科技专项(19226351D);国家重点研发计划项目(2017YFD0101000);中国农业科学院创新工程项目

Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat

HUANG Yi-Wen1(), DAI Xu-Ran1, LIU Hong-Wei1, YANG Li1, MAI Chun-Yan2, YU Li-Qiang3, YU Guang-Jun3, ZHANG Hong-Jun1,*(), LI Hong-Jie1,*(), ZHOU Yang1,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Engineering Laboratory for Crop Molecular Breeding, Beijing 100081, China
    2Center for Technological Innovation of Dwarf-male-sterile Wheat Breeding (Xinxiang), Xinxiang 453731, Henan, China
    3Zhaoxian Experiment Station, Shijiazhuang Academy of Agricultural and Forestry Sciences, Zhaoxian 051530, Hebei, China
  • Received:2020-11-20 Accepted:2021-03-19 Published:2021-11-12 Published online:2021-04-01
  • Contact: ZHANG Hong-Jun,LI Hong-Jie,ZHOU Yang
  • Supported by:
    Science and Technology Project for Modern Seed Industry of Hebei(19226351D);National Key Research and Development Program of China(2017YFD0101000);Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences

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摘要：

Ppo-A1和Ppo-D1是控制小麦多酚氧化酶(polyphenol oxidase, PPO)活性的关键基因。有报道PPO活性与穗发芽抗性有关, 但Ppo-A1和Ppo-D1位点不同等位变异对穗发芽抗性的影响尚不明确。本研究利用248份我国主栽小麦品种3年发芽指数, 结合Ppo-A1和Ppo-D1位点等位变异分型结果, 研究两个位点不同等位变异及其等位变异组合与穗发芽抗性的关系。结果表明, 发芽指数主要受年份、Ppo-A1位点和Ppo-A1 × Ppo-D1互作共同影响。在Ppo-A1位点, 携带低PPO活性等位变异Ppo-A1b的小麦品种发芽指数显著低于携带高PPO活性等位变异Ppo-A1a的品种, 平均发芽指数相差5.22%; 相反, 在Ppo-D1位点携带低PPO活性等位变异Ppo-D1a品种的发芽指数高于携带高PPO活性等位变异Ppo-D1b的品种, 但差异不显著。在4种等位变异组合中, Ppo-A1bPpo-D1b组合类型品种的发芽指数最低。上述Ppo-A1位点等位变异与穗发芽抗性的关系在轮选13 × 济麦20 F2及F2:3分离群体中得到验证。PPO活性和Ppo-A1相对表达量均与发芽指数呈显著正相关。本研究表明, Ppo-A1b等位变异的分子标记可以有效地用于穗发芽抗性辅助选择。

关键词: 小麦, 抗穗发芽育种, 多酚氧化酶, Ppo-A1b等位变异, 分子标记辅助选择

Abstract:

Ppo-A1 and Ppo-D1 are the major genes that control the activity of polyphenol oxidase (PPO) in wheat. It has been reported that the activity of polyphenol oxidase affects pre-harvest sprouting (PHS) resistance, but the effect of different alleles/allelic combinations at the Ppo-A1 and Ppo-D1 loci on PHS resistance remains unclear. The current study was carried out to elucidate the effects based on the germination index obtained from 248 Chinese wheat cultivars in a three-year trial in combination with genotypic data at the Ppo-A1 and Ppo-D1 loci. Analysis of variation for the Ppo-A1 and Ppo-D1 loci showed that year, Ppo-A1 locus and Ppo-A1 × Ppo-D1 interaction had significant effects on germination index. At locus Ppo-A1, germination index of cultivars carrying the allele Ppo-A1b of low PPO activity was 5.22% lower than that carrying the allele Ppo-A1a of high PPO activity on average. In contrast, the cultivars carrying the allele Ppo-D1a of low PPO activity had higher germination index than that carrying the allele Ppo-D1b of high PPO activity at locus Ppo-D1, but no significant differences between two alleles. Among the four allelic combinations, the cultivars with the Ppo-A1bPpo-D1b had the lowest germination index. The relationship between the alleles at locus Ppo-A1 and PHS resistance had been verified in the Lunxuan 13 × Jimai 20 F2 and F2:3 segregation populations. There were significantly positive correlations between PPO activity / relative expression level of Ppo-A1 gene and germination index. This study suggests that functional markers of allele Ppo-A1b can be effectively applied in marker-assisted selection for PHS resistance.

Key words: Triticum aestivum, the breeding for resistance to pre-harvest sprouting, polyphenol oxidase, Ppo-A1b allele, molecular marker-assisted selection

表1

Ppo-A1和Ppo-D1位点不同等位变异检测的功能标记信息"

位点
Locus		 标记
Marker		 引物序列
Primer sequence (5′-3′)		 等位基因
Allele		 扩增片段
Amplified fragment (bp)		 退火温度
Annealing temperature (℃)		 参考文献
Reference
Ppo-A1 PPO18 F: AACTGCTGGCTCTTCTTCCCA Ppo-A1a 685 60 [25]
R: AAGAAGTTGCCCATGTCCGC Ppo-A1b 876
Ppo-D1 PPO16 F: TGCTGACCGACCTTGACTCC Ppo-D1a 713 60 [26]
R: CTCGTCACCGTCACCCGTAT
PPO29 F: TGAAGCTGCCGGTCATCTAC Ppo-D1b 490
R: AAGTTGCCCATGTCCTCGCC

表2

qRT-PCR分析引物"

基因
Gene		 上游引物序列
Forward primer sequence (5′-3′)		 下游引物序列
Reverse primer sequence (5′-3′)
Ppo-A1 GCAACTGCCAAACGCCCGAGC CAGCACGAAGTCGGTGATGCC
Ppo-D1 CCGGACCTTGAGATCCAGGTG. GAAGGTGTCGTCGCCGATGAG
Actin GTTTCCTGGAATTGCTGATCGCAT CATTATTTCATACAGCAGGCAAGC

表3

2018-2020年248份小麦品种发芽指数方差分析"

变异来源Source of variation 自由度DF 均方Mean square
基因型 Genotypes (G) 247 0.26**
年份 Years (Y) 2 10.91**
基因型 × 年份互作G × Y interaction 494 0.03**
重复 Replications 2 0.04
误差 Error 1486 0.01

图1

小麦品种年份间发芽指数相关分析 **表示在P < 0.01水平下差异显著。"

表4

248份小麦品种和轮选13 × 济麦20群体平均发芽指数、标准差、变幅和变异系数"

品种/群体
Cultivar/population		 年份
Year		 均值±标准差
Mean ± SD		 变幅
Range		 变异系数
Coefficient of variation
248份小麦品种 2018 47.46 ± 19.48 5.33-93.71 40.97
248 wheat cultivars 2019 58.39 ± 17.61 17.14-92.19 30.10
2020 34.21 ± 19.05 2.95-88.71 55.59
平均 Mean 46.70 ± 17.03 11.55-88.70 36.41
轮选13 × 济麦20群体 2019 (F2) 59.49 ± 16.59 18.10-90.76 27.88
Lunxuan 13 × Jimai 20 population 2020 (F2:3) 46.94 ± 13.22 19.14-79.53 28.18

图2

亲本轮选13 (A)和济麦20 (B)穗发芽抗性比较(发芽3 d)"

图3

分子标记PPO18、PPO16和PPO29检测部分小麦品种Ppo-A1 (A)和Ppo-D1 (B)位点的不同等位变异 M: DNA ladder 2000; 1: 京411; 2: 洛旱7号; 3: 山农20; 4: 石4185; 5: 石麦12; 6: 新麦20; 7: 开麦20; 8: 许农5号; 9: 淮麦20; 10: 邢麦13。"

表5

248份小麦品种Ppo-A1和Ppo-D1位点3年发芽指数方差分析"

变异来源
Source of variation		 自由度
DF		 均方
Mean square
年份 Years (Y) 2 3.543**
Ppo-A1 1 0.512**
Ppo-D1 1 0.009
Ppo-A1 × Ppo-D1互作Ppo-A1 × Ppo-D1 interaction 1 0.173*
Ppo-A1 × 年份互作 Ppo-A1 × Y interaction 2 0.010
Ppo-D1 × 年份互作 Ppo-D1 × Y interaction 2 0.001
Ppo-A1 × Ppo-D1 × 年份互作 Ppo-A1 × Ppo-D1 × Y interaction 2 0.010
误差 Error 732 0.035

图4

小麦品种Ppo-A1 (A)和Ppo-D1 (B)位点不同等位变异间发芽指数比较 **表示在P < 0.01水平下差异显著。圆点: 极端值。"

图5

小麦品种Ppo-A1和Ppo-D1位点不同等位变异组合间发芽指数比较 不同字母表示等位变异间发芽指数在0.01水平显著。圆点: 极端值。"

图6

轮选13 × 济麦20群体Ppo-A1位点不同等位变异间发芽指数比较 不同字母表示等位变异间发芽指数在0.01水平显著。"

图7

不同基因型品种(A)和轮选13 × 济麦20群体F2:3株系(B) PPO活性比较 不同字母表示基因型间发芽指数或者PPO活性在0.01水平显著。"

图8

不同基因型品种(A和B)和轮选13 × 济麦20群体F2:3株系(C) Ppo-A1和Ppo-D1基因实时定量表达分析 不同字母表示基因型间发芽指数或者基因相对表达水平在0.01水平显著。"

[1] Li H J, Zhou Y, Xin W L, Wei Y Q, Zhang J L, Guo L L. Wheat breeding in northern China: achievements and technical advances. Crop J, 2019, 7: 718-729.
doi: 10.1016/j.cj.2019.09.003
[2] Ali A, Cao J J, Jiang H, Chang C, Zhang H P, Sheikh S W, Shah L, Ma C X. Unraveling molecular and genetic studies of wheat (Triticum aestivum L.) resistance against factors causing pre-harvest sprouting. Agronomy, 2019, 9: 117.
doi: 10.3390/agronomy9030117
[3] Depauw R M, Knox R E, Singh A K, Fox S L, Humphreys D G, Hucl P. Developing standardized methods for breeding pre-harvest sprouting resistant wheat, challenges and successes in Canadian wheat. Euphytica, 2012, 188: 7-14.
doi: 10.1007/s10681-011-0611-y
[4] Liu C X, Ding F, Hao F H, Yu M, Lei H H, Wu X Y, Zhao Z X, Guo H X, Yin J, Wang Y L. Reprogramming of seed metabolism facilitates pre-harvest sprouting resistance of wheat. Sci Rep, 2016, 6: 20593.
doi: 10.1038/srep20593
[5] Zhu Y L, Wang S X, Wei W X, Xie H Y, Liu K, Zhang C, Wu Z Y, Jiang H, Cao J J, Zhao L X. Genome-wide association study of pre-harvest sprouting tolerance using a 90K SNP array in common wheat (Triticum aestivum L.). Theor Appl Genet, 2019, 132: 2947-2963.
doi: 10.1007/s00122-019-03398-x
[6] Liu Y J, Liu Y X, Zhou Y, Wight C, Pu Z, Qi P F, Jiang Q T, Deng M, Wang Z X, Wei Y M. Conferring resistance to pre-harvest sprouting in durum wheat by a QTL identified in Triticum spelta. Euphytica, 2017, 213: 19.
doi: 10.1007/s10681-016-1796-x
[7] Shao M Q, Bai G H, Rife T W, Jesse P, Lin M, Liu S B, Cai H, Tadele K, Allan F, Harold T. QTL mapping of pre-harvest sprouting resistance in a white wheat cultivar Danby. Theor Appl Genet, 2018, 131: 1683-1697.
doi: 10.1007/s00122-018-3107-5
[8] Vetch J M, Stougaard R N, Martin J M, Giroux M J. Revealing the genetic mechanisms of pre-harvest sprouting in hexaploid wheat (Triticum aestivum L.). Plant Sci, 2019, 281: 180-185.
doi: 10.1016/j.plantsci.2019.01.004
[9] Zhang Y J, Xia X C, He Z H. The seed dormancy allele TaSdr-A1a associated with pre-harvest sprouting tolerance is mainly present in Chinese wheat landraces. Theor Appl Genet, 2017, 130: 81-89.
doi: 10.1007/s00122-016-2793-0
[10] Nakamura S, Abe F, Kawahigashi H, Nakazono K, Tagiri A, Matsumoto T, Utsugi S, Ogawa T, Handa H, Ishida H. A wheat homolog of MOTHER OF FT AND TFL1 acts in the regulation of germination. Plant Cell, 2011, 23: 3215-3229.
doi: 10.1105/tpc.111.088492
[11] Himi E, Noda K. Red grain colour gene (R) of wheat is a Myb-type transcription factor. Euphytica, 2005, 143: 239-242.
doi: 10.1007/s10681-005-7854-4
[12] Yang G H, Zhao X Q, Li B, Liu J Z, Li Z S. Molecular cloning and characterization of a DFR from developing seeds of blue-grained wheat in anthocyanin biosynthetic pathway. Acta Bot Sin, 2003, 45: 1329-1338.
[13] Bailey P C, Mckibbin R S, Lenton J R, Holdsworth M J, Flintham J E, Gale M D. Genetic map locations for orthologous Vp1 genes in wheat and rice. Theor Appl Genet, 1999, 98: 281-284.
doi: 10.1007/s001220051069
[14] Torada A, Koike M, Ogawa T, Takenouchi Y, Tadamura K, Wu J, Matsumoto T, Kawaura K, Ogihara Y. A causal gene for seed dormancy on wheat chromosome 4A encodes a MAP kinase kinase. Curr Biol, 2016, 26: 782-787.
doi: 10.1016/j.cub.2016.01.063
[15] Lin M, Liu S B, Zhang G R, Bai G H. Effects of TaPHS1 and TaMKK3-A genes on wheat pre-harvest sprouting resistance. Agronomy, 2018, 8: 210.
doi: 10.3390/agronomy8100210
[16] 吴玉良, 杨煜峰. 无原花色素大麦突变体穗发芽的研究. 浙江农业大学学报, 1996, 22: 647-650.
Wu Y L, Yang Y F. Study on pre-harvest sprouting of barley mutants without proanthocyanidins. J Zhejiang Agric Univ, 1996, 22: 647-650 (in Chinese with English abstract).
[17] Weidner S, Krupa U, Amarowicz R, Karamac M, Abe S. Phenolic compounds in embryos of triticale caryopses at different stages of development and maturation in normal environment and after dehydration treatment. Euphytica, 2002, 126: 115-122.
doi: 10.1023/A:1019607302792
[18] 魏海蓉, 高东升, 李宪利. 植物生长调节剂对甜樱桃休眠的调控及花芽酚类物质含量的影响. 园艺学报, 2005, 32: 17-21.
Wei H R, Gao D S, Li X L. Effects of plant growth regulators on the content of phenolics in sweet cherry dormant flower buds and dormancy. Acta Hortic Sin, 2005, 32: 17-21 (in Chinese with English abstract).
[19] 宋亮, 潘开文, 王进闯, 马玉红. 酚酸类物质对苜蓿种子萌发及抗氧化物酶活性的影响. 生态学报, 2006, 10: 3393-3403.
Song L, Pan K W, Wang J C, Ma Y H. Effects of phenolic acids on seed germination and seedling antioxidant enzyme activity of alfalfa. Acta Ecol Sin, 2006, 10: 3393-3403 (in Chinese with English abstract).
[20] Esmaeili N, Ebrahimzadeh H, Abdi K. Correlation between polyphenol oxidase (PPO) activity and total phenolic contents in Crocus sativus L. Corms during dormancy and sprouting stages. Pharmacogn Mag, 2017, 13: S519-S524.
[21] Demeke T, Morris C F, Campbell K G, King G E, Anderson J A, Chang H G. Wheat polyphenol oxidase: distribution and genetic mapping in three inbred line populations. Crop Sci, 2001, 41: 1750-1757.
doi: 10.2135/cropsci2001.1750
[22] Baik B, Czuchajowska Z, Pomeranz Y. Comparison of polyphenol oxidase activities in wheats and flours from Australian and US cultivars. J Cereal Sci, 1994, 19: 291-296.
doi: 10.1006/jcrs.1994.1036
[23] 王宝凤, 付金锋, 董立峰. 多酚氧化酶活性与小麦抗穗发芽的关系及抗穗发芽新品种秦麦3号的选育. 麦类作物学报, 2006, 29: 97-100.
Wang B F, Fu J F, Dong L F. The Relationship between activity of polyphenol oxidase and resistance to pre-harvest sprouting in wheat and breeding of Qinmai 3 with high resistance to PHS. J Triticeae Crops, 2006, 29: 97-100 (in Chinese with English abstract).
[24] 王宝凤, 杨雪, 付金锋, 董立峰, 马理. 低酚酶活性选择对小麦穗发芽的影响. 核农学报, 2015, 29: 899-907.
Wang F B, Yang X, Fu J F, Dong L F, Ma L. Effects of consecutive selection for low polyphenol oxidase activity on wheat pre-harvest sprouting. J Nucl Agric Sci, 2015, 29: 899-907 (in Chinese with English abstract).
[25] Sun D J, He Z H, Xia X C, Zhang L P, Morris C F, Appels R, Ma W J, Wang H. A novel STS marker for polyphenol oxidase activity in bread wheat. Mol Breed, 2005, 16: 209-218.
doi: 10.1007/s11032-005-6618-0
[26] He X Y, He Z H, Zhang L P, Sun D J, Morris C F, Fuerst E P, Xia X C. Allelic variation of polyphenol oxidase (PPO) genes located on chromosomes 2A and 2D and development of functional markers for the PPO genes in common wheat. Theor Appl Genet, 2007, 115: 47-58.
pmid: 17426955
[27] 买春艳, 李洪杰, 刘宏伟, 杨丽, 于立强, 周阳, 张宏军. 北方冬麦区小麦品种产量相关性状和幼穗分化特点研究. 麦类作物学报, 2018, 7: 773-781.
Mai C Y, Li H J, Liu H W, Yang L, Yu L Q, Zhou Y, Zhang H J. Characterization of yield related traits and inflorescence differentiation in representative wheat cultivars from the northern China winter wheat region. J Triticeae Crops, 2018, 7: 773-781 (in Chinese with English abstract).
[28] Martinez S A, Godoy J, Huang M, Zhang Z W, Carter A H, Garland Campbell K A, Steber C M. Genome-wide association mapping for tolerance to pre-harvest sprouting and low falling numbers in wheat. Front Plant Sci, 2018, 9: 141.
doi: 10.3389/fpls.2018.00141 pmid: 29491876
[29] Anderson J V, Morris C F. An improved whole-seed assay for screening wheat germplasm for polyphenol oxidase activity. Crop Sci, 2001, 41: 1697-1705.
doi: 10.2135/cropsci2001.1697
[30] Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 2001, 25: 402-408.
pmid: 11846609
[31] Jin H, Wen W E, Liu J D, Zhai S N, Zhang Y, Yan J, Liu X Y, Xia X C, He Z H. Genome-wide QTL mapping for wheat processing quality parameters in a Gaocheng 8901/Zhoumai 16 recombinant inbred line population. Front Plant Sci, 2016, 7: 1032.
[32] 张立平, 葛秀秀, 何中虎, 王德森, 闫俊, 夏先春, Sutherland M W. 普通小麦多酚氧化酶活性的QTL分析. 作物学报, 2005, 31: 7-10.
Zhang L P, Ge X X, He Z H, Wang D S, Yan J, Xia X C, Sutherland M W. Mapping QTLs for polyphenol oxidase activity in a DH population from common wheat. Acta Agron Sin, 2005, 31: 7-10 (in Chinese with English abstract).
[33] Nilthong S, Graybosch R A, Baenziger P S. Inheritance of grain polyphenol oxidase (PPO) activity in multiple wheat (Triticum aestivum L.) genetic backgrounds. Theor Appl Genet, 2012, 125: 1705-1715.
doi: 10.1007/s00122-012-1947-y
[34] 张晓, 高德荣, 李曼, 刘大同, 吴素兰, 江伟, 吕国锋. 小麦面粉和鲜面片色泽及Psy-A1与Ppo-A1等位变异检测. 麦类作物学报, 2019, 39: 415-422.
Zhang X, Gao D R, Li M, Liu D T, Wu S L, Jiang W, Lyu G F. Color of flour and fresh dough sheet of wheat varieties and detection of allelic variations for genes Psy-A1 and Ppo-A1. J Triticeae Crops, 2019, 39: 415-422 (in Chinese with English abstract).
[35] Kato K, Maruyama-Funatsuki W, Yanaka M, Ban Y, Takata K. Improving preharvest sprouting resistance in durum wheat with bread wheat genes. Breed Sci, 2017, 67: 466-471.
doi: 10.1270/jsbbs.17042
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