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作物学报 ›› 2021, Vol. 47 ›› Issue (11): 2080-2090.doi: 10.3724/SP.J.1006.2021.01089

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

小麦多酚氧化酶基因Ppo-A1和Ppo-D1位点等位变异与穗发芽抗性的关系

黄义文1(), 代旭冉1, 刘宏伟1, 杨丽1, 买春艳2, 于立强3, 于广军3, 张宏军1,*(), 李洪杰1,*(), 周阳1,*()   

  1. 1中国农业科学院作物科学研究所 / 作物分子育种国家工程实验室, 北京 100081
    2新乡县矮败小麦育种技术创新中心, 河南新乡 453731
    3石家庄市农林科学研究院赵县实验基地, 河北赵县 051530
  • 收稿日期:2020-11-20 接受日期:2021-03-19 出版日期:2021-11-12 网络出版日期:2021-04-01
  • 通讯作者: 张宏军,李洪杰,周阳
  • 作者简介:E-mail: 18838916683@163.com
  • 基金资助:
    河北省现代种业科技专项(19226351D);国家重点研发计划项目(2017YFD0101000);中国农业科学院创新工程项目

Relationship between the allelic variations at the Ppo-A1 and Ppo-D1 loci and pre-harvest sprouting resistance in wheat

HUANG Yi-Wen1(), DAI Xu-Ran1, LIU Hong-Wei1, YANG Li1, MAI Chun-Yan2, YU Li-Qiang3, YU Guang-Jun3, ZHANG Hong-Jun1,*(), LI Hong-Jie1,*(), ZHOU Yang1,*()   

  1. 1Institute of Crop Sciences, Chinese Academy of Agricultural Sciences / National Engineering Laboratory for Crop Molecular Breeding, Beijing 100081, China
    2Center for Technological Innovation of Dwarf-male-sterile Wheat Breeding (Xinxiang), Xinxiang 453731, Henan, China
    3Zhaoxian Experiment Station, Shijiazhuang Academy of Agricultural and Forestry Sciences, Zhaoxian 051530, Hebei, China
  • Received:2020-11-20 Accepted:2021-03-19 Published:2021-11-12 Published online:2021-04-01
  • Contact: ZHANG Hong-Jun,LI Hong-Jie,ZHOU Yang
  • Supported by:
    Science and Technology Project for Modern Seed Industry of Hebei(19226351D);National Key Research and Development Program of China(2017YFD0101000);Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences

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摘要：

Ppo-A1和Ppo-D1是控制小麦多酚氧化酶(polyphenol oxidase, PPO)活性的关键基因。有报道PPO活性与穗发芽抗性有关, 但Ppo-A1和Ppo-D1位点不同等位变异对穗发芽抗性的影响尚不明确。本研究利用248份我国主栽小麦品种3年发芽指数, 结合Ppo-A1和Ppo-D1位点等位变异分型结果, 研究两个位点不同等位变异及其等位变异组合与穗发芽抗性的关系。结果表明, 发芽指数主要受年份、Ppo-A1位点和Ppo-A1 × Ppo-D1互作共同影响。在Ppo-A1位点, 携带低PPO活性等位变异Ppo-A1b的小麦品种发芽指数显著低于携带高PPO活性等位变异Ppo-A1a的品种, 平均发芽指数相差5.22%; 相反, 在Ppo-D1位点携带低PPO活性等位变异Ppo-D1a品种的发芽指数高于携带高PPO活性等位变异Ppo-D1b的品种, 但差异不显著。在4种等位变异组合中, Ppo-A1bPpo-D1b组合类型品种的发芽指数最低。上述Ppo-A1位点等位变异与穗发芽抗性的关系在轮选13 × 济麦20 F2及F2:3分离群体中得到验证。PPO活性和Ppo-A1相对表达量均与发芽指数呈显著正相关。本研究表明, Ppo-A1b等位变异的分子标记可以有效地用于穗发芽抗性辅助选择。

关键词: 小麦, 抗穗发芽育种, 多酚氧化酶, Ppo-A1b等位变异, 分子标记辅助选择

Abstract:

Ppo-A1 and Ppo-D1 are the major genes that control the activity of polyphenol oxidase (PPO) in wheat. It has been reported that the activity of polyphenol oxidase affects pre-harvest sprouting (PHS) resistance, but the effect of different alleles/allelic combinations at the Ppo-A1 and Ppo-D1 loci on PHS resistance remains unclear. The current study was carried out to elucidate the effects based on the germination index obtained from 248 Chinese wheat cultivars in a three-year trial in combination with genotypic data at the Ppo-A1 and Ppo-D1 loci. Analysis of variation for the Ppo-A1 and Ppo-D1 loci showed that year, Ppo-A1 locus and Ppo-A1 × Ppo-D1 interaction had significant effects on germination index. At locus Ppo-A1, germination index of cultivars carrying the allele Ppo-A1b of low PPO activity was 5.22% lower than that carrying the allele Ppo-A1a of high PPO activity on average. In contrast, the cultivars carrying the allele Ppo-D1a of low PPO activity had higher germination index than that carrying the allele Ppo-D1b of high PPO activity at locus Ppo-D1, but no significant differences between two alleles. Among the four allelic combinations, the cultivars with the Ppo-A1bPpo-D1b had the lowest germination index. The relationship between the alleles at locus Ppo-A1 and PHS resistance had been verified in the Lunxuan 13 × Jimai 20 F2 and F2:3 segregation populations. There were significantly positive correlations between PPO activity / relative expression level of Ppo-A1 gene and germination index. This study suggests that functional markers of allele Ppo-A1b can be effectively applied in marker-assisted selection for PHS resistance.

Key words: Triticum aestivum, the breeding for resistance to pre-harvest sprouting, polyphenol oxidase, Ppo-A1b allele, molecular marker-assisted selection

表1

Ppo-A1和Ppo-D1位点不同等位变异检测的功能标记信息"

位点
Locus		 标记
Marker		 引物序列
Primer sequence (5′-3′)		 等位基因
Allele		 扩增片段
Amplified fragment (bp)		 退火温度
Annealing temperature (℃)		 参考文献
Reference
Ppo-A1 PPO18 F: AACTGCTGGCTCTTCTTCCCA Ppo-A1a 685 60 [25]
R: AAGAAGTTGCCCATGTCCGC Ppo-A1b 876
Ppo-D1 PPO16 F: TGCTGACCGACCTTGACTCC Ppo-D1a 713 60 [26]
R: CTCGTCACCGTCACCCGTAT
PPO29 F: TGAAGCTGCCGGTCATCTAC Ppo-D1b 490
R: AAGTTGCCCATGTCCTCGCC

表2

qRT-PCR分析引物"

基因
Gene		 上游引物序列
Forward primer sequence (5′-3′)		 下游引物序列
Reverse primer sequence (5′-3′)
Ppo-A1 GCAACTGCCAAACGCCCGAGC CAGCACGAAGTCGGTGATGCC
Ppo-D1 CCGGACCTTGAGATCCAGGTG. GAAGGTGTCGTCGCCGATGAG
Actin GTTTCCTGGAATTGCTGATCGCAT CATTATTTCATACAGCAGGCAAGC

表3

2018-2020年248份小麦品种发芽指数方差分析"

变异来源Source of variation 自由度DF 均方Mean square
基因型 Genotypes (G) 247 0.26**
年份 Years (Y) 2 10.91**
基因型 × 年份互作G × Y interaction 494 0.03**
重复 Replications 2 0.04
误差 Error 1486 0.01

图1

小麦品种年份间发芽指数相关分析 **表示在P < 0.01水平下差异显著。"

表4

248份小麦品种和轮选13 × 济麦20群体平均发芽指数、标准差、变幅和变异系数"

品种/群体
Cultivar/population		 年份
Year		 均值±标准差
Mean ± SD		 变幅
Range		 变异系数
Coefficient of variation
248份小麦品种 2018 47.46 ± 19.48 5.33-93.71 40.97
248 wheat cultivars 2019 58.39 ± 17.61 17.14-92.19 30.10
2020 34.21 ± 19.05 2.95-88.71 55.59
平均 Mean 46.70 ± 17.03 11.55-88.70 36.41
轮选13 × 济麦20群体 2019 (F2) 59.49 ± 16.59 18.10-90.76 27.88
Lunxuan 13 × Jimai 20 population 2020 (F2:3) 46.94 ± 13.22 19.14-79.53 28.18

图2

亲本轮选13 (A)和济麦20 (B)穗发芽抗性比较(发芽3 d)"

图3

分子标记PPO18、PPO16和PPO29检测部分小麦品种Ppo-A1 (A)和Ppo-D1 (B)位点的不同等位变异 M: DNA ladder 2000; 1: 京411; 2: 洛旱7号; 3: 山农20; 4: 石4185; 5: 石麦12; 6: 新麦20; 7: 开麦20; 8: 许农5号; 9: 淮麦20; 10: 邢麦13。"

表5

248份小麦品种Ppo-A1和Ppo-D1位点3年发芽指数方差分析"

变异来源
Source of variation		 自由度
DF		 均方
Mean square
年份 Years (Y) 2 3.543**
Ppo-A1 1 0.512**
Ppo-D1 1 0.009
Ppo-A1 × Ppo-D1互作Ppo-A1 × Ppo-D1 interaction 1 0.173*
Ppo-A1 × 年份互作 Ppo-A1 × Y interaction 2 0.010
Ppo-D1 × 年份互作 Ppo-D1 × Y interaction 2 0.001
Ppo-A1 × Ppo-D1 × 年份互作 Ppo-A1 × Ppo-D1 × Y interaction 2 0.010
误差 Error 732 0.035

图4

小麦品种Ppo-A1 (A)和Ppo-D1 (B)位点不同等位变异间发芽指数比较 **表示在P < 0.01水平下差异显著。圆点: 极端值。"

图5

小麦品种Ppo-A1和Ppo-D1位点不同等位变异组合间发芽指数比较 不同字母表示等位变异间发芽指数在0.01水平显著。圆点: 极端值。"

图6

轮选13 × 济麦20群体Ppo-A1位点不同等位变异间发芽指数比较 不同字母表示等位变异间发芽指数在0.01水平显著。"

图7

不同基因型品种(A)和轮选13 × 济麦20群体F2:3株系(B) PPO活性比较 不同字母表示基因型间发芽指数或者PPO活性在0.01水平显著。"

图8

不同基因型品种(A和B)和轮选13 × 济麦20群体F2:3株系(C) Ppo-A1和Ppo-D1基因实时定量表达分析 不同字母表示基因型间发芽指数或者基因相对表达水平在0.01水平显著。"

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