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作物学报 ›› 2016, Vol. 42 ›› Issue (05): 627-632.doi: 10.3724/SP.J.1006.2016.00627

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

ω-黑麦碱基因沉默对小麦1B/1R易位系加工品质的影响

柴建芳*,王海波*,马秀英,张翠绵,董福双   

  1. 河北省农林科学院遗传生理研究所 / 河北省植物转基因中心, 河北石家庄 050051
  • 收稿日期:2015-09-28 修回日期:2016-03-02 出版日期:2016-05-12 网络出版日期:2016-03-11
  • 通讯作者: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • 基金资助:

    本项目由河北省自然科学基金项目(C2014301005)资助。

Effect of ω-Secalin Gene Silencing on Processing Quality of Wheat 1B/1R Translocation Line

CHAI Jian-Fang*,WANG Hai-Bo*,MA Xiu-Ying,ZHANG Cui-Mian,DONG Fu-Shuang   

  1. Genetics and Physiology Institute, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China
  • Received:2015-09-28 Revised:2016-03-02 Published:2016-05-12 Published online:2016-03-11
  • Contact: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Hebei Province, China (C2014301005).

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摘要：

ω-黑麦碱是造成小麦1B/1R易位系加工品质差的一个重要因素, 为探索解决这一问题, 构建了ω-黑麦碱基因沉默表达载体, 并通过农杆菌介导转化小麦品种金禾9123, 获得3个T0代转基因植株, 再经连续的扩繁和PCR检测, 获得纯合转基因T4代株系。酸性PAGE检测结果表明, 这些纯合转基因株系中ω-黑麦碱的总表达量平均下降53%。这些ω-黑麦碱基因沉默的转基因株系的面筋指数、沉降值和稳定时间均显著提高, 而其农艺性状, 如株高、穗粒数、千粒重和小区产量, 均没有受到不良影响, 说明沉默ω-黑麦碱基因可以在不影响产量的前提下提高小麦1B/1R易位系的加工品质。

关键词: 小麦1B/1R易位系, 黑麦碱, RNA干扰, 基因沉默, 加工品质

Abstract:

Omega-secalin is an important factor for the poor processing quality of wheat 1B/1R translocation lines. In this study, an expression vector of silencing ω-secalin gene was constructed and transferred into wheat variety Jinhe 9123 via Agrobacterium tumefaciens strain EH105. The three T0 transgenic plants obtained were consecutively multiplied after PCR validation until the T4 lines. Acid-PAGE assay indicated that total amount of ω-secalins in the transgenic lines decreased 53% on average compared with that in their respective control. In the three homozygous transgenic lines, gluten index, precipitation value, and stabilization time increased significantly, whereas the agronomic traits, such as plant height, grain number per spike, 1000-grain weight and plot yield, had no significant changes compared with those in the wild type. These results suggest that silencing ω-secalin genes can improve the processing quality of wheat 1B/1R translocation lines on the premise of not affecting grain yield.

Key words: Wheat 1B, 1R translocation line, Secalin, RNAi, Gene silencing, Processing quality

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