欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2016, Vol. 42 ›› Issue (05): 627-632.doi: 10.3724/SP.J.1006.2016.00627

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

ω-黑麦碱基因沉默对小麦1B/1R易位系加工品质的影响

柴建芳*,王海波*,马秀英,张翠绵,董福双   

  1. 河北省农林科学院遗传生理研究所 / 河北省植物转基因中心, 河北石家庄 050051
  • 收稿日期:2015-09-28 修回日期:2016-03-02 出版日期:2016-05-12 网络出版日期:2016-03-11
  • 通讯作者: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • 基金资助:

    本项目由河北省自然科学基金项目(C2014301005)资助。

Effect of ω-Secalin Gene Silencing on Processing Quality of Wheat 1B/1R Translocation Line

CHAI Jian-Fang*,WANG Hai-Bo*,MA Xiu-Ying,ZHANG Cui-Mian,DONG Fu-Shuang   

  1. Genetics and Physiology Institute, Hebei Academy of Agriculture and Forestry Sciences / Plant Genetic Engineering Center of Hebei Province, Shijiazhuang 050051, China
  • Received:2015-09-28 Revised:2016-03-02 Published:2016-05-12 Published online:2016-03-11
  • Contact: 柴建芳, E-mail: chai87652130@163.com; 王海波, E-mail: nkywanghb@163.com
  • Supported by:

    This study was supported by the Natural Science Foundation of Hebei Province, China (C2014301005).

RichHTML

PDF

1927

被引次数

4

摘要：

ω-黑麦碱是造成小麦1B/1R易位系加工品质差的一个重要因素, 为探索解决这一问题, 构建了ω-黑麦碱基因沉默表达载体, 并通过农杆菌介导转化小麦品种金禾9123, 获得3个T0代转基因植株, 再经连续的扩繁和PCR检测, 获得纯合转基因T4代株系。酸性PAGE检测结果表明, 这些纯合转基因株系中ω-黑麦碱的总表达量平均下降53%。这些ω-黑麦碱基因沉默的转基因株系的面筋指数、沉降值和稳定时间均显著提高, 而其农艺性状, 如株高、穗粒数、千粒重和小区产量, 均没有受到不良影响, 说明沉默ω-黑麦碱基因可以在不影响产量的前提下提高小麦1B/1R易位系的加工品质。

关键词: 小麦1B/1R易位系, 黑麦碱, RNA干扰, 基因沉默, 加工品质

Abstract:

Omega-secalin is an important factor for the poor processing quality of wheat 1B/1R translocation lines. In this study, an expression vector of silencing ω-secalin gene was constructed and transferred into wheat variety Jinhe 9123 via Agrobacterium tumefaciens strain EH105. The three T0 transgenic plants obtained were consecutively multiplied after PCR validation until the T4 lines. Acid-PAGE assay indicated that total amount of ω-secalins in the transgenic lines decreased 53% on average compared with that in their respective control. In the three homozygous transgenic lines, gluten index, precipitation value, and stabilization time increased significantly, whereas the agronomic traits, such as plant height, grain number per spike, 1000-grain weight and plot yield, had no significant changes compared with those in the wild type. These results suggest that silencing ω-secalin genes can improve the processing quality of wheat 1B/1R translocation lines on the premise of not affecting grain yield.

Key words: Wheat 1B, 1R translocation line, Secalin, RNAi, Gene silencing, Processing quality

[1] 周阳, 何中虎, 张改生, 夏兰琴, 陈新民, 高永超, 井赵斌, 于广军. 1BL/1RS易位系在我国小麦育种中的应用. 作物学报, 2004,30: 531–535
Zhou Y, He Z H, Zhang G S, Xia L Q, Chen X M, Gao Y C, Jing Z B, Yu G J. Utilization of 1BL/1RS translocation in wheat breeding in China. Acta Agron Sin, 2004, 30: 531–535 (in Chinese with English abstract)

[2] 杨足君, 任正隆. 抗白粉病基因Pm8在四川小麦中遗传表达初步研究. 四川农业大学学报, 1997, 15: 452–456
Yang Z J, Ren Z L. Expression of gene Pm8 for resistance to powdery mildew in wheat for Sichuan. J. Sichuan Agric Univ, 1997, 15: 452–456 (in Chinese with English abstract)
[3] Shi Z X, Chen X M, Line R F, Leung H, Wellings C R. Development of resistance gene analog polymorphism markers for the Yr9 gene resistance to wheat stripe rust. Genome, 2001, 44: 509–516
[4] Lei M P, Li G R, Liu C, Yang Z J. Characterization of wheat-Secale africanum introgression lines reveals evolutionary aspects of chromosome 1R in rye. Genome, 2012, 55: 765–774
[5] Yang M Y, Ren T H, Yan B J, Li Z, Ren Z L. Diversity resistance to Puccinia striiformis f. sp tritici in rye chromosome arm 1RS expressed in wheat. Genet Mol Res, 2014, 13: 8783–8793
[6] Dhaliwal A S, Mares D J, Marshall D R. Measurement of dough surface stickness associated with 1B/1R chromosome translocation in bread wheats. Cereal Sci, 1990, 12: 165–175
[7] 刘建军, 何中虎, Peña R J, 赵振东. 1BL/1RS易位对小麦加工品质的影响. 作物学报, 2004, 30: 149–153
Liu J J, He Z H, Peña R J, Zhao Z D. The effects of 1B/1R translocation on grain quality and noodle quality of bread wheat. Acta Agron Sin, 2004, 30: 149–153 (in Chinese with English abstract)
[8] Graybosh R A, Peterson C J, Hansen L E, Mattern P J. Relationships between protein solubility characteristics, 1BL/1RS, high molecular weight glutenin composition, and end-use quality in winter wheat germ plasm. Cereal Chem, 1990, 67: 342–349
[9] Lukaszewski A J. Manipulation of the 1RS?1BL translocation in wheat by induced homoeologous recombination. Crop Sci, 2000, 40: 216–225
[10] Fu S, Tang Z, Ren Z, Zhang H. Transfer to wheat (Triticum aestivum) of small chromosome segments from rye (Secale cereale) carrying disease resistance genes. J Appl Genet, 2010, 51: 115–121
[11] Scott M H, Amy A C, Gregory J H. Post-transcriptional gene silencing by double-stranded RNA. Nat Rev Genet, 2001, 2: 110–119
[12] Phillip A S, RNA interference. Genes & Develop, 2001, 15: 485–490
[13] Li J R, Zhao W, Li Q Z, Ye X G, An B Y, Li X, Zhang X S. RNA silencing of waxy gene results in low levels of amylose in the seeds of transgenic wheat (Triticum aestivum L.). Acta Genet Sin, 2005, 32: 846–854
[14] 孙重霞, 杨凤萍, 张婷, 隋晓燕, 梁荣奇, LIU Qing, 张晓东, 李保云. 利用RNAi技术抑制籽粒PPO合成改良小麦面粉白度的研究. 中国农业科学, 2013, 46: 1104–1113
Sun C X, Yang F P, Zhang T, Sui X Y, Liang R Q, Liu Q, Zhang X D, Li B Y. Down-regulation of the expression of grain ppo genes to improve wheat dough whiteness by RNA interference. Sci Agric Sin, 2013, 46: 1104–1113 (in Chinese with English abstract)
[15] Chai J F, Liu X, Jia J Z. Homoeologous cloning of ω-secalin gene family in a wheat 1BL/1RS translocation. Cell Res, 2005, 15: 658–664
[16] Murai J, Taira T, Ohta D. Isolation and characterization of the three Waxy genes encoding the granule-bound starch synthase in hexaploid wheat. Gene, 1999, 234: 71–79
[17] 张学勇醇溶蛋白电泳在小麦种质资源遗传分析中的应用. 中国农业科学, 1995, 28(4): 25–32
Zhang X Y, Yang X M, Dong Y C. Genetic analysis of wheat germplasm by acid polyacrylamide gel electrophoresis of gliadins. Sci Agric Sin, 1995, 28(4): 25–32 (in Chinese with English abstract), 杨欣明, 董玉琛.
[18] 纪军, 刘冬成, 王静, 李俊明, 张爱民. 一种小麦高、低分子量麦谷蛋白亚基的提取方法. 遗传, 2008, 30: 123–126
Ji J, Liu D C, Wang J, Li J M, Zhang A M. A method of extraction and separation of wheat gluten. Hereditas (Beijing), 2008, 30: 123–126 (in Chinese with English abstract)
[19] Gil-Humanes J, Pistón F, Tollefsen S, Sollid L M, Barro F. Effective shutdown in the expression of celiac disease-related wheat gliadin T-cell epitopes by RNA interference. Proc Natl Acad Sci USA, 2010, 107: 17023–17028
[20] Hussain A, and Lukow O M. Characterization of the 1B/1R translocation in wheat using water extractable protein concentrate. Euphytica, 1994, 78: 109–113
[21] Sharma S, Bhat P R, Ehdaie B, Close T J, Lukaszewski A J, Waines J G. Integrated genetic map and genetic analysis of a region associated with root traits on the short arm of rye chromosome 1 in bread wheat. Theor Appl Genet,2009, 119: 783–793
[22] 相微微, 张怀刚, 王道文, 柳觐, 刘宝龙. 转基因小麦沉默的HMW-GS基因的遗传及表达. 麦类作物学报, 2009, 29: 185–188
Xiang W W, Zhang H G, Wang D W, Liu J, Liu B L. Inheritance and expression of silenced HMW-GS genes in wheat. J Triticeae Crops, 2009, 29: 185–188 (in Chinese with English abstract)
[23] 武茹, 高德荣, 别同德, 张晓, 赵芸, 程顺和. 转基因小麦1Dx5基因沉默的遗传表达和品质效应分析. 麦类作物学报, 2010, 30: 991–996
Wu R, Gao D R, Bie T D, Zhang X, Zhao Y, Cheng S H. Genetic analysis and quality effects of 1Dx5 subunit silent in transgenic wheat. J Triticeae Crops, 2010, 30: 991–996 (in Chinese with English abstract)
[1] 苏强,荣玮,张增艳. 小麦类受体蛋白激酶基因TaPK3A的克隆与抗纹枯病功能初步分析[J]. 作物学报, 2019, 45(8): 1158-1165.
[2] 王旭虹,李鸣晓,张群,金峰,马秀芳,姜树坤,徐正进,陈温福. 籼型血缘对籼粳稻杂交后代产量和加工及外观品质的影响[J]. 作物学报, 2019, 45(4): 538-545.
[3] 周香艳, 杨江伟, 唐勋, 文义凯, 张宁, 司怀军. amiRNA技术沉默C-3氧化酶编码基因StCPD对马铃薯抗旱性的影响[J]. 作物学报, 2018, 44(04): 512-521.
[4] 方雅洁，朱亚军，吴志超，陈凯，申聪聪，石英尧，徐建龙. 全基因组关联定位籼稻种质资源外观和加工品质QTL[J]. 作物学报, 2018, 44(01): 32-42.
[5] 程伟,李和平,何水林,廖玉才. 寄主诱导的基因沉默提高植物真菌病害抗性研究进展[J]. 作物学报, 2017, 43(08): 1115-1121.
[6] 杨向东,牛陆,张伟,杨静,杜茜,邢国杰,郭东全,李启云,董英山*. RNAi介导SMV-P3基因沉默增强大豆对花叶病毒病的抗性[J]. 作物学报, 2016, 42(11): 1647-1655.
[7] 张平平,马鸿翔*,姚金保,周淼平,张鹏. 高分子量谷蛋白单亚基缺失对软质小麦宁麦9号加工品质的影响[J]. 作物学报, 2016, 42(05): 633-640.
[8] 周露, 沈贝贝, 白苏阳, 刘喜, 江玲, 翟虎渠, 万建民. 以RNA干扰γ-氨基丁酸转氨酶1基因(OsGABA-T1)表达提高稻米γ-氨基丁酸(GABA)含量[J]. 作物学报, 2015, 41(09): 1305-1312.
[9] 胡丹丹,张帆,黄立钰,卓大龙,张帆,周永力,石英尧,黎志康. 胁迫相关蛋白激酶基因OsSAPK2调控水稻抗白叶枯病反应[J]. 作物学报, 2015, 41(08): 1191-1200.
[10] 任琴,王亚军,郭志鸿,李继平,谢忠奎,王若愚,王立,惠娜娜. 植物介导的RNA干扰引起马铃薯晚疫病菌基因的沉默[J]. 作物学报, 2015, 41(06): 881-888.
[11] 郭保卫，朱大伟，朱聪聪，许轲，周兴涛，张洪程，陈厚存，张军，陈京都，戴其根，霍中洋，魏海燕，曹利强，李明银. 有序摆抛栽对粳型超级稻稻米品质的影响[J]. 作物学报, 2015, 41(03): 487-498.
[12] 陈坤梅,李宏伟,林凡云,陈耀锋,李滨,郑琪,李振声. 利用病毒介导基因沉默方法研究小麦抗光氧化相关基因[J]. 作物学报, 2014, 40(11): 1905-1913.
[13] 董剑,杨华,赵万春,李晓燕,陈其皎,高翔. 普通小麦中国春–簇毛麦易位系T1DL•1VS和T1DS•1VL的农艺和品质特性[J]. 作物学报, 2013, 39(08): 1386-1390.
[14] 刘光快,曹珍珍,韦克苏,潘刚,苏达,张春娇,程方民. 水稻蛋白二硫键异构酶基因沉默载体构建及其转基因后代的高温结实特性分析[J]. 作物学报, 2013, 39(05): 816-826.
[15] 张勇，申小勇，张文祥，陈新民，阎俊，张艳，王德森，王忠伟，刘悦芳，田宇兵，夏先春，何中虎. 高分子量谷蛋白5+10亚基和1B/1R易位分子标记辅助选择在小麦品质育种中的应用[J]. 作物学报, 2012, 38(10): 1743-1751.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2