作物学报 ›› 2017, Vol. 43 ›› Issue (12): 1767-1773.
张晓琼,王晓雯,田维江,张孝波,孙莹,李杨羊,谢佳,何光华,桑贤春*
ZHANG Xiao-Qiong, WANG Xiao-Wen, TIAN Wei-Jiang, ZHANG Xiao-Bo, Sun Ying, LI Yang-Yang, Xie Jia, HE Guang-Hua, and SANG Xian-Chun*
摘要:
叶夹角的大小直接影响水稻叶面积指数,进而调控群体光合作用,是水稻株型育种中重要的指标,研究其发育机制对水稻株型育种具有重要的意义。利用EMS诱变籼型水稻保持系西大1B,获得一个植株散生且叶夹角变大的突变体s524。田间种植条件下,苗期s524的叶夹角极显著大于野生型;分蘖期s524的分蘖角极显著增大,株型松散;成熟期s524整个植株呈匍匐状生长。而野生型株型在整个生育期均保持相对紧凑,叶夹角较小。石蜡切片分析显示,s524叶夹角增大是由叶枕近轴面细胞变大造成的。s524的主要农艺性状与野生型相比无明显变化。遗传分析表明该性状受1对隐性核基因控制,利用SSR标记进行基因定位,最终将S524定位在第11染色体标记RM4746和RM26742之间324 kb的物理范围内,包含散生基因LAZY1。测序结果显示s524突变体在LAZY1第3外显子上发生了一个T到C的碱基替换,导致第143位氨基酸由野生型的缬氨酸突变为丙氨酸,表明s524是一个新的LAZY1等位突变体。s524对外源油菜素内酯(BR)的敏感性降低,BR信号传导途径关键基因BU1在s524中的表达上调了近10倍,早期研究表明BU1基因的过表达可导致叶夹角变大。推测LAZY1/S524可能通过BR信号传导途径调控水稻叶夹角的发育。
[1]Li Z K, Paterson A H, Pinson S R, Stansel J W. RFLP facilitated analysis of tiller and leaf angles in rice (Oryza sativa L.). Euphytica, 1999, 109(6): 79–84 [2]钱前, 何平, 滕胜, 曾大力, 朱立煌. 水稻分蘖角度的QTLs分析. 遗传学报, 2001, 28: 29–32 Qian Q, He P, Teng S, Zeng D L, Zhu L H. QTLs analysis of tiller angle in rice (Oryza sativa L.). Acta Genet Sin, 2001, 28: 29–32 (in Chinese with English abstract) [3]余传元, 刘裕强, 江玲, 王春明, 翟虎渠, 万建民. 水稻分蘖角度的QTL定位和主效基因的遗传分析. 遗传学报, 2005, 32: 948–954 Yu C Y, Liu Y Q, Jiang L, Wang C M, Zhai H Q, Wan J M. QTLs mapping and genetic analysis of tiller angle in rice (Oryza sativa L.). Acta Genet Sin, 2005, 32: 948–954 (in Chinese with English abstract) [4]Yu B, Lin Z, Li H, Li X, Li J, Wang Y, Zhang X, Zhu Z, Zhai W, Wang X, Xie D, Sun C. TAC1, a major quantitative trait locus controlling tiller angle in rice. Plant J, 2007, 52: 891–898 [5]Li P, Wang Y, Qian Q, Fu Z, Wang M, Zeng D, Li B, Wang X, Li J. LAZY1 controls rice shoot gravitropism through regulating polar auxin transport. Cell Res, 2007, 17: 402–410 [6]Yoshihara T, Lino M. Identification of the gravitropism-related rice gene LAZY1 and elucidation of LAZY1 dependent and independent gravity signaling pathways. Plant Cell Physiol, 2007, 48: 678–688 [7]Zhang C, Xu Y Y, Guo S Y, Zhu J Y, Huan Q, Liu H H, Wang L, Luo G Z, Wang X J, Chong K. Dynamics of brassinosteroid response modulated by negative regulator LIC in rice. PLoS Genet, 2012, 8(4): e1002686 [8]Zhao S Q, Xiang J J, Xue H W. Studies on the rice LEAF INCLINATION1 (LC1), an IAA-amido synthetase, reveal the effects of auxin in leaf Inclination control. Mol Plant, 2013, 6: 174–187 [9]Zhao S Q, Hu J, Guo L B, Qian Q, Xue H W. Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res, 2010, 20: 935–947 [10]Zhang S N, Wang S K, Xu Y X, Yu C L, Shen C J, Qian Q, Geisler M, Jiang D A, Qi Y H. The auxin response factor, OsARF19, controls rice leaf angles through positively regulating OsGH3-5 and OsBRI1. Plant Cell Environ, 2015, 38: 638–654 [11]Wu X R, Tang D, Li M, Wang K J, Cheng Z K. Loose plant architecture 1, an indeterminate domain protein involved in shoot gravitropism, regulates plant architecture in rice. Plant Physiol, 2013, 161: 317–329 [12]Ning J, Zhang B C, Wang N L, Wang N, Zhou Y H, Xiong L Z. Increased leaf angle 1, a Raf-Like MAPKKK that interacts with a nuclear protein family, regulates mechanical tissue formation in the lamina joint of rice. Plant Cell, 2011, 23: 4334–4347 [13]Sang X C, Li Y F, Luo Z K, Ren D Y, Fang L K, Wang N, Zhao F M, Ling Y H, Yang Z L, Liu Y S, He G H. CHIMERIC FLORAL ORGANS1, encoding a monocot-specific MADS box protein, regulates floral organ identity in rice. Plant Physiol, 2012, 160: 788–807 [14]Porebski S, Bailey L.G, Baum B.R. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Rep, 1997, 15: 8–15 [15]Tanaka A, Nakagawa H, Tomita C, Shimatani Z, Ohtake M, Nomura T, Jiang C J, Dubouzet J G, Kikuchi S, Sekimoto H, Yokota T, Asami T, Kamakura T, Mori M. BRASSINOSTEROID UPREGULATED1, encoding a helix-loop-helix Protein, is a novel gene involved in brassinosteroid signaling and controls bending of the lamina joint in rice. Plant Physiol, 2009, 151: 669–680 [16]Bai M Y, Zhang L Y, Gampala S S, Zhu S W, Song W Y, Chong K, Wang Z Y. Functions of OsBZR1 and 14-3-3 proteins in brassinosteroid signaling in rice. Proc Natl Acad Sci USA, 2007, 104: 13839–13844 [17]Zhang L Y, Bai M Y, Wu J, Zhu J Y, Wang H, Zhang Z, Wang W, Sun Y, Zhao J, Sun X, Yang H, Xu Y, Kim S H, Fujioka S, Lin W H, Chong K, Lu T, Wang Z Y. Antagonistic HLH/bHLH transcription factors mediate brassinosteroid regulation of cell elongation and plant development in rice and arabidopsis. Plant Cell, 2009, 21: 3767–3780 [18]Hu X M, Qian Q, Xu T, Zhang Y E, Dong G J, Gao T, Xie Q,?Xue Y B. The U-box E3 ubiquitin ligase TUD1 functions with a heterotrimeric g α subunit to regulate brassinosteroid-mediated growth in rice. PLoS Genet, 2013, 9(3): e1003391 [19]Sakamoto T, Morinaka Y, Ohnishi T, Sunohara H, Fujioka S, Ueguchi-Tanaka M, Mizutani M, Sakata K, Takatsuto S, Yoshida S, Tanaka H, Kitano H, Matsuoka M. Erect leaves caused by brassinosteroid deficiency increase biomass production and grain yield in rice. Nat Biotechnol, 2006, 24: 105–109 [20]Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S,?Ashikari M,?Kitano H, Matsuok M. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome p450. Plant Cell, 2003, 15: 2900–2910 [21]Sakamoto T, Morinaka Y, Inukai Y, Kitano H, Fujioka S. Auxin signal transcription factor regulates expression of the brassinosteroid receptor gene in rice. Plant J, 2013, 73: 676–688 [22]Sun S, Chen D, Li X, Qiao S, Shi C, Li C, Shen H, Wang X. Brassinosteroid signaling regulates leaf erectness in Oryza sativa via the control of a specific U-type cyclin and cell proliferation. Dev Cell, 2015, 34: 220–228 [23]Duan K, Li L, Hu P, Xu S P, Xu Z H, Xue H W. A brassinolide-suppressed rice MADS-box transcription factor, OsMDP1, has a negative regulatory role in BR signaling. Plant J, 2006, 47: 519–531 |
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