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作物学报 ›› 2009, Vol. 35 ›› Issue (2): 279-285.doi: 10.3724/SP.J.1006.2009.00279

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗水稻纹枯病qSB-9Tq基因效应及作用方式分析

殷跃军;左示敏**;王辉;张亚芳;陈宗祥;马玉银;顾世梁;潘学彪   

  1. 扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室，江苏扬州225009
  • 收稿日期:2008-07-18 修回日期:2008-10-10 出版日期:2009-02-12 网络出版日期:2008-12-11
  • 通讯作者: 潘学彪
  • 基金资助:

    本研究由国家技术研究发展计划（863计划）项目（2006AA10Z165，2006AA10A103），公益性行业（农业）科研专项经费（nyhyzx07-049)资助

Effect and Action Analysis of qSB-9Tq Conferring Resistance to Rice Sheath Blight

YIN Yue-Jun,ZUO Shi-Min**,WANG Hui,ZHANG Ya-Fang,CHEN Zong-Xiang,MA Yu-Yin,GU Shi-Liang,PAN Xue-Biao*   

  1. Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genetics,Ministry of Education,Yangzhou University, Yangzhou 22509,china
  • Received:2008-07-18 Revised:2008-10-10 Published:2009-02-12 Published online:2008-12-11
  • Contact: PAN Xue-Biao

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1553

被引次数

18

摘要：

水稻第9染色体上存在1个抗纹枯病QTL，被命名为qSB-9，水稻品种特青在该QTL上携带抗性等位基因qSB-9Tq，而Lemont携带相对感病等位基因qSB-9Le。为精确地评价qSB-9Tq的抗病效应，分析其作用方式，利用分子标记进行前景选择和背景选择，从轮回亲本Lemont与特青回交后代群体中筛选到1个目标单株。连续3年对该单株的扩繁后代(BC6F2)及随后获得的近等基因系采用嵌入法进行接种鉴定试验。田间试验采取2种不同的设计。第一种是完全随机试验，即从BC6F2分离群体中筛选出目标区间为qSB-9TqTq纯合型、qSB-9LeLe纯合型和qSB-9TqLe杂合型个体，并对3种基因型个体间的病级平均数差异进行统计分析。第二种设计为随机区组设计，即在BC6F3和BC6F4代，分别对上述3种基因型的近等基因系群体，按3次重复的随机区组设计进行移栽和接种鉴定试验。结果表明，3年的试验结果表现出一致的趋势，即qSB-9Tq存在于分子标记RM242~Y92.5之间，可减轻病级1.0级(0~9级病情分级系统)左右，且其抗性表现为几乎完全的显性特征。本研究的结果为qSB-9Tq的精细定位和育种利用奠定了基础。

关键词: 水稻(Oryza sativa L.), 纹枯病, QTL, 近等基因系, 标记辅助选择, 抗性效应；作用方式

Abstract:

A quantitative trait locus (QTL) contributing partial resistance to rice sheath blight (SB), named as qSB-9, has been confirmed on chromosome 9 of rice. A rice cultivar, Teqing, possesses the relatively resistant allele, qSB-9Tq, and Lemont has the relatively susceptible allele, qSB-9Le. To evaluate the resistance effect and action mode of qSB-9Tq accurately, a BC6F1 plant, from a backcross of Lemont (recurrent parent)/Teqing, with heterozygous genotype of qSB-9TqLe and similar genetic background to Lemont was screened out by using marker-assisted selection. Artificial inoculation with strong pathogenic Rhizoctonia solani line, RH-9, was conducted on the BC6F2 population and the near-isogenic lines (NILs) selected from BC6F3 and BC6F4 generation in a three-year test. Two experimental designs were adopted. One was completely randomized experiment, in which the plants with three genotypes (qSB-9TqTq, qSB-9LeLe, and qSB-9TqLe) on the locus of qSB-9 were distinguished by detecting marker genotypes flanking the qSB-9 in BC6F2 segregation population. The other was a randomized block design with 3 replicates for three NILs in BC6F3 and BC6F4 generations. Results from the two experiments all suggested that the qSB-9Tq was a dominant resistance allele in the interval between the markers of RM242 and Y92.5, and could reduce disease rating about 1.0 score under ‘0–9’ SB-disease rating system. The result of the research laid a strong foundation for the fine mapping and breeding utilization of the qSB-9Tq.

Key words: Rice(Oryza sativa L.), Sheath blight, QTL, Near-isogenic line, Marker-assisted selection, Resistant effect, Gene action

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