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作物学报 ›› 2018, Vol. 44 ›› Issue (04): 614-619.doi: 10.3724/SP.J.1006.2018.00614

• 研究简报 • 上一篇    下一篇

玉米大斑病广谱抗性外引自交系的发掘与抗病基因初步鉴定

肖明纲1,2(), 宋凤景3, 孙兵2, 左辛4, 赵广山5, 辛爱华2, 李柱刚2,*()   

  1. 1黑龙江省农业科学院博士后科研工作站, 黑龙江哈尔滨 150086
    2黑龙江省农业科学院耕作栽培研究所/黑龙江省寒地作物生理生态实验室/黑龙江省作物分子设计与种质创新重点实验室, 黑龙江哈尔滨 150086
    3青岛市农业科学研究院, 山东青岛 266109
    4黑龙江省农业科学院农村能源研究所, 黑龙江哈尔滨 150086
    5佳木斯市农业技术推广总站, 黑龙江佳木斯 154002
  • 收稿日期:2017-08-30 接受日期:2018-01-08 出版日期:2018-01-30 网络出版日期:2018-01-30
  • 通讯作者: 李柱刚
  • 作者简介:

    xiaoyang8076@163.com

  • 基金资助:
    本研究由黑龙江省博士后基金项目(LB-Z14186)资助

Exploration of Foreign Maize Inbred Lines with Broad Spectrum Resistance to Northern Corn Leaf Blight and Preliminary Identification of Resistance Genes

Ming-Gang XIAO1,2(), Feng-Jing SONG3, Bing SUN2, Xin ZUO4, Guang-Shan ZHAO5, Ai-Hua XIN2, Zhu-Gang LI2,*()   

  1. 1 Postdoctoral Programme, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    2 Heilongjiang Province Cold Region Crop Physiology and Ecology Laboratory / Key Laboratory of Crop Molecular Design and Germplasm Innovation in Heilongjiang Province Tillage and Cultivation Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    3 Qingdao Academy of Agricultural Science, Qingdao 266109, Shandong, China
    4 Ruaral Energy Sources Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, Heilongjiang, China
    5 Agricultural Technology Extension Station of Jiamusi City, Jiamusi 154002, Heilongjiang, China
  • Received:2017-08-30 Accepted:2018-01-08 Published:2018-01-30 Published online:2018-01-30
  • Contact: Zhu-Gang LI
  • Supported by:
    This study was supported by Heilongjiang Postdoctoral Fund (LB-Z14186).

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摘要：

从2014—2016年连续3年对43份来自美国、法国、俄罗斯和德国的玉米资源进行了抗大斑病人工接种鉴定, 筛选到高抗玉米大斑病材料7份, 抗病材料1份, 中抗材料6份, 抗性材料占鉴定总材料的比例为32.6%。利用F2群体, 对7份高抗材料进行了大斑病抗性遗传分析, 抗感植株分离比例和适合性测验证明, 自交系A04、F02、F05和R01对大斑病的抗性可能是由一对显性基因控制的。抗谱分析表明, 自交系A04、F02、F05和R01携带的抗大斑病基因不同于Ht1、Ht2、Ht3和HtN, 可能是新的抗病基因。该研究结果可为今后我国玉米大斑病抗性种质的引进及改良提供重要参考。

关键词: 玉米自交系, 大斑病, 抗性评价, 遗传分析

Abstract:

From 2014 to 2016, forty-three maize inbred lines introduced from the United States, France, Russia and Germany, were identified and evaluated for resistance to northern corn leaf blight (NCLB). The results showed that seven, one and six maize inbreds were highly resistant, resistant or moderately resistant to NCLB, accounting for 32.6%. Exserohilum turcicum was used to test the F2 populations for analyzing the inheritance of NCLB resistance in the seven highly resistance maize inbred lines. Genetic analysis suggested that A04, F02, F05, and R01 of these inbred lines probably carried a single dominant gene conferring their resistance to NCLB. The reaction patterns to isolates 0, 1, 2, N, and 123N of E. turcicum showed that new genes resistance to NCLB were most likely present in A04, F02, F05, and R01. The results provided useful information on the introduction of maize germplasms and genetic improvement for resistance to NCLB.

Key words: maize inbred lines, northern corn leaf blight, resistance evaluation, inheritance

图1

外引材料2014年大斑病抗性级别分布 HR: 高抗; R: 抗; MR: 中抗; S: 感; HS: 高感。"

图2

外引材料高抗和高感大斑病田间表型 A04、G03: 自交系; HR: 高抗; HS: 高感。"

表1

14份外引材料对玉米大斑病多年抗性鉴定"

自交系
Inbred lines		 来源
Origin		 2014 2015 2016
病斑面积
Lesion area
(%)		 抗性评价
Resistance
evaluation		 病斑面积
Lesion area
(%)		 抗性评价
Resistance
evaluation		 病斑面积
Lesion area
(%)		 抗性评价
Resistance
evaluation
A02 美国America 15 MR 10 R 17 MR
A04 美国America 1 HR 1 HR 1 HR
A05 美国America 12 MR 12 MR 12 MR
A08 美国America 23 MR 20 MR 25 MR
A11 美国America 5 HR 5 HR 5 HR
A18 美国America 11 MR 11 MR 10 R
A20 美国America 25 MR 25 MR 20 MR
A24 美国America 0 HR 0 HR 0 HR
A28 美国America 13 MR 15 MR 10 R
F02 法国France 1 HR 3 HR 3 HR
F03 法国France 9 R 7 R 9 R
F05 法国France 2 HR 1 HR 1 HR
R01 俄罗斯Russia 4 HR 4 HR 4 HR
G02 德国Germany 0 HR 1 HR 0 HR

表2

7个F2群体对大斑凸脐蠕孢的抗性反应"

杂交组合
Cross		 抗病植株数
R		 感病植株数
S		 分离比
χ2		 P值
P-value
获白/A04 Huobai/A04 448 132 1.5540 0.2125
获白/A11 Huobai/A11 416 168 4.4201 0.0355
获白/A24 Huobai/A24 400 160 3.8095 0.0510
获白/F02 Huobai/F02 438 130 1.3521 0.2449
获白/F05 Huobai/F05 438 142 0.0828 0.7736
获白/R01 Huobai/R01 454 158 0.2179 0.6407
获白/G02 Huobai/G02 432 172 3.8940 0.0485

表3

4份玉米自交系对0、1、2、N和123N的反应型"

自交系
Inbred line		 小种鉴定结果Identification of species
0 1 2 N 123N
A619 S S S S S
A619Ht1 R S R R S
A619Ht2 R R S R S
A619Ht3 R R R R S
A619HtN R R R S S
A04 R R R R R
F02 R R R R R
F05 R R R R R
R01 R R R R R
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