作物学报 ›› 2018, Vol. 44 ›› Issue (11): 1640-1649.doi: 10.3724/SP.J.1006.2018.01640
尚维1,赵申清玉1,党江波1,郭启高1,梁国鲁1,*(),杨超2,张艳2,陈益银2
Wei SHANG1,Shen-Qing-Yu ZHAO1,Jiang-Bo DANG1,Qi-Gao GUO1,Guo-Lu LIANG1,*(),Chao YANG2,Yan ZHANG2,Yi-Yin CHEN2
摘要:
以烟草(Nicotiana tabacum)品种云烟87的八倍体(2n = 8x = 96)和野生烟草N. plumbaginifolia (2n = 2x = 20)的基因组DNA为模板, 对340对烟草SSR引物进行筛选以获得能扩增多态性条带的引物。利用多态性引物对种间杂交后代及190株回交后代的基因组DNA进行扩增, 并对N. plumbaginifolia中的SSR标记的连锁情况进行简要分析。经筛选获得了多态性引物29对。结果显示, 在190株后代中, 159株的基因组DNA能扩增出N. plumbaginifolia的特异SSR位点, 可以判定该159株为N. tabacum的N. plumbaginifolia异源染色体植株, 其余31株植株可能不含有N. plumbaginifolia的染色体。经UPGMA聚类分析, 本群体中植株的遗传多样性较为丰富, 部分分子标记在后代中的出现具有完全相关性。29个标记中14个可确定来源于5条不同染色体, N. plumbaginifolia的29个位点在回交后代中的扩增效率并不相同, 且效率均较低(低于31.00%), 说明该杂种中N. plumbaginifolia基因组的垂直传递效率较低。利用SSR分子标记可以判定云烟87八倍体与N. plumbaginifolia杂交获得的后代为真杂种, 且自该远缘杂种回交后代中筛选获得大量异源染色体植株。这些结果和筛选获得异源染色体植株为进一步创制N. tabacum-N. plumbaginifolia抗黑胫病单体附加系以及易位系奠定了基础。
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