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作物学报 ›› 2020, Vol. 46 ›› Issue (4): 484-490.doi: 10.3724/SP.J.1006.2020.94112

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

抗青枯病兼大果和高出仁率的花生新种质创制

李威涛,徐志军,蔡岩,郭建斌,喻博伦,黄莉,陈玉宁,周小静,罗怀勇,刘念,陈伟刚,任小平,姜慧芳()   

  1. 中国农业科学院油料作物研究所 / 农业部油料作物生物学与遗传育种重点实验室, 湖北武汉 430062
  • 收稿日期:2019-08-04 接受日期:2019-12-26 出版日期:2020-04-12 网络出版日期:2020-01-15
  • 通讯作者: 姜慧芳
  • 作者简介:E-mail: 965301631@qq.com
  • 基金资助:
    本研究由国家自然科学基金项目资助(31601340);本研究由国家自然科学基金项目资助(31571713);本研究由国家自然科学基金项目资助(31761143005);本研究由国家自然科学基金项目资助(31461143022)

Development of novel peanut genotypes with resistance to bacterial wilt disease, large pod, and high shelling percentage

LI Wei-Tao,XU Zhi-Jun,CAI Yan,GUO Jian-Bin,YU Bo-Lun,HUANG Li,CHEN Yu-Ning,ZHOU Xiao-Jing,LUO Huai-Yong,LIU Nian,CHEN Wei-Gang,REN Xiao-Ping,JIANG Hui-Fang()   

  1. Oil Crops Research Institute, China Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs, Wuhan 430062, Hubei, China
  • Received:2019-08-04 Accepted:2019-12-26 Published:2020-04-12 Published online:2020-01-15
  • Contact: Hui-Fang JIANG
  • Supported by:
    This study was supported by the National Natural Science Foundation of China(31601340);This study was supported by the National Natural Science Foundation of China(31571713);This study was supported by the National Natural Science Foundation of China(31761143005);This study was supported by the National Natural Science Foundation of China(31461143022)

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摘要：

青枯病是影响花生产量和品质的重要土传性细菌病害, 百果重和出仁率是与花生产量相关的重要性状。本研究利用远杂9102和徐州68-4杂交构建的RIL群体, 在B02染色体上定位到青枯病抗性主效QTL qBWRB02。结合前期对百果重和出仁率QTL的定位结果发现, 所涉及的3个性状的主效QTL分布在不同的染色体上。以RIL群体基因型数据和多个环境的青枯病抗性、百果重和出仁率表型数据为基础, 利用与主效QTL紧密连锁分子标记筛选出6份聚合抗青枯病、荚果大、出仁率高3种优良性状的新种质, 可以作为育种中间材料或亲本培育高产抗病新品种。本研究利用分子标记辅助选择和表型鉴定相结合有效筛选抗病高产种质, 为未来花生育种提供了新思路。

关键词: 花生新种质, 青枯病抗性, 百果重, 出仁率

Abstract:

Bacterial wilt is an important soil-borne bacterial disease effecting yield and quality of peanut. Hundred pod weight and shelling percentage are also related to peanut yield. In this study, the major QTL qBWRB02 related to bacterial wilt resistance was identified by using the RIL population derived from the cross between Yuanza 9102 and Xuzhou 68-4. Combining with the QTL mapping results for shelling percentage and hundred pod weight in previous research, it was found that the major QTL for these three traits were distributed on different chromosomes. Based on genotype data of RIL population and phenotypic data of bacterial wilt resistance, hundred pod weight and shelling percentage in multi-environment, We screened out six novel accessions with resistance to bacterial wilt disease, large pod, and high shelling percentage from RIL population by molecular markers closely linked with major QTL, which can be used as breeding intermediates or parents in peanut breeding for high yield and high disease resistance. The results of effectively screening disease-resistant and high-yield germplasm through the combination of molecular marker-assisted selection and phenotypic identification provide a new idea for peanut breeding in the future.

Key words: novel peanut genotype, bacterial wilt diseases resistance, hundred pod weight, shelling percentage

表1

利用QTLNetwork 2.0检测到的青枯病抗性QTL及参数"

QTL名称
QTL name		 环境
Environment		 范围
Range (cM)		 加性效应
Additive effect		 贡献率
PVE (%)
(A)		 贡献率
PVE (%)
(AE)
qBWRA01.1 2013HA 17.4-18.7 -9.5732 16.57
qBWRA01.2 ME 17.4-18.7 -6.16 8.77 0.52
(qBWRA01)
qBWRB02.1 2013HA 91.5-92.5 -15.95 21.45
qBWRB02.2 2014NC 91.5-92.5 -19.51 27.76
qBWRB02.3 2015NC 91.5-92.5 -21.18 45.21
qBWRB02.4 ME 91.5-92.5 -18.44 27.87 0.22
(qBWRB02)
qBWRB05 ME 4.5-8.5 5.66 2.54 0.79
qBWRB10.1 2013HA 87.9-98.9 -14.79 8.36
qBWRB10.2 2015NC 87.9-101.9 -11.81 6.49
qBWRB10.3 ME 90.9-100.9 -10.30 3.80 0.35
(qBWRB10)

图1

抗青枯病QTL在遗传图谱上的位置 方框内为主效QTL。"

表2

不同青枯病抗性的品系和品种中分子标记AHGS2344的基因型"

材料 Material 亲本 Parent AHGS2344 表型 Phenotype
远杂9102 Yuanza 9102 — A 抗 Resistant
中花21 Zhonghua 21 远杂9102 Yuanza 9102 A 抗 Resistant
J8 远杂9102 Yuanza 9102 A 抗 Resistant
J11 远杂9102 Yuanza 9102 A 抗 Resistant
J64 远杂9102 Yuanza 9102 A 抗 Resistant
J91 远杂9102 Yuanza 9102 A 抗 Resistant
J92 远杂9102 Yuanza 9102 A 抗 Resistant
J12 远杂9102 Yuanza 9102 B 不抗 Susceptible
中花6号 Zhonghua 6 — A 抗 Resistant
QT1319 中花6号Zhonghua 6 A 抗 Resistant
QT1315 中花6号Zhonghua 6 A 抗 Resistant
QT1299 中花6号Zhonghua 6 B 不抗 Susceptible
QT1322 中花6号Zhonghua 6 B 不抗 Susceptible
QT1306 中花6号Zhonghua 6 B 不抗 Susceptible
QT1314 中花6号Zhonghua 6 B 不抗 Susceptible
徐花13 Xuhua 13 — B 不抗 Susceptible
徐州68-4 Xuzhou 68-4 — B 不抗 Susceptible

表3

通过与QTL紧密连锁的分子标记创制的兼具3种优良性状的新种质"

家系
Line		 青枯病抗性(qBWB02)
Resistance to bacterial wilt (qBWB02)		 百果重(qHPWA05)
Hundred pod weight (qHPWA05)		 出仁率(qSPA09)
Shelling percentage (qSPA09)
AhTE0775 AHGS2344 均值
Mean (%)		 AD05A20499 Ad05A20262 均值
Mean (g)		 AGGS0957 GNB652 均值
Mean (%)
QT0718 A A 81.89 B B 239.09 A A 81.77
QT0719 A A 80.36 B B 257.07 A A 80.19
QT0748 A A 92.88 B B 238.22 A A 83.68
QT0752 A A 94.89 B B 228.44 A A 79.41
QT0793 A A 88.10 B B 235.49 A A 81.63
QT0845 A A 88.89 B B 222.45 A A 80.83
徐州68-4
Xuzhou 68-4		 B B 20.00 B B 251.84 B B 76.93
远杂9102
Yuanza 9102		 A A 89.80 A A 187.43 A A 81.84
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