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作物学报 ›› 2017, Vol. 43 ›› Issue (05): 701-707.doi: 10.3724/SP.J.1006.2017.00701

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

花生出仁率QTL分析及其与荚果大小的相关性

蔡岩,徐志军,李振动,李新平,郭建斌,任小平,黄莉,陈伟刚,陈玉宁,周小静,罗怀勇,姜慧芳*   

  1. 中国农业科学院油料作物研究所 / 农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062
  • 收稿日期:2016-06-22 修回日期:2016-11-03 出版日期:2017-05-12 网络出版日期:2016-12-14
  • 通讯作者: 姜慧芳, E-mail: peanut@oilcrops.cn, Tel: 027-86711550
  • 基金资助:

    本研究由国家自然科学基金项目(31271764, 31371662, 31471534, 31461143022), 农业部农作物种质资源保护项目(2015NWB035)和国家现代农业产业技术体系建设专项(CARS-14-花生种质资源评价)资助。

Quantitative Trait Locus Shelling Percentage and Correlation Between Shelling Percentage with Pod Traits in Cultivated Peanut (A. hypogaea L.)

CAI Yan,XU Zhi-Jun,LI Zhen-Dong,LI Xin-Ping,GUO Jian-Bin,REN Xiao-Ping,HUANG Li,CHEN Wei-Gang,CHEN Yu-Ning,ZHOU Xiao-Jing,LUO Huai-Yong,JIANG Hui-Fang*   

  1. Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences / Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
  • Received:2016-06-22 Revised:2016-11-03 Published:2017-05-12 Published online:2016-12-14
  • Contact: Jiang Huifang, E-mail: peanut@oilcrops.cn, Tel: 027-86711550
  • Supported by:

    This study was supported by the National Natural Science Foundation of China (31271764, 31371662, 31471534, 31461143022), the Crop Germplasm Resources Protection Project (2015NWB035), and the China Agriculture Research System (CARS-14-peanut resource evaluation).

摘要:

花生是重要的油料作物,成熟花生的出仁率不仅与花生的产油量相关,还与果壳厚度及脱壳难易程度相关,是花生遗传改良的重要成分。本研究以远杂9102×徐州68-4杂交后代衍生的重组自交系(RIL)的188个家系为材料,2013—2014年连续2年考察出仁率和荚果表型,发现有29份材料的出仁率稳定高于高值亲本远杂9102。出仁率与荚果长、荚果宽、荚果厚和百果重之间呈显著或极显著负相关。利用已构建的包含365个标记22个连锁群的遗传连锁图,采用WinQTLcart 2.5软件的复合区间作图法对出仁率进行QTL定位和效应估计,2年共检测到22个出仁率QTL,遗传贡献率为2.75%~13.49%,其中2年重复检测到的区间有5个(AHGS0344~AGGS2438、AGGS0957~AHGA7048、AGGS0058~AHGA72558、AHTE0446~AHGA363492和AGGS0311~AGGS2287),分布在连锁群LG02、LG03和LG10上,遗传贡献率为3.61%~13.49%。结合前期对该群体荚果大小的定位结果,有4个与出仁率相关的区间同时存在荚果大小QTL,其遗传效应均相反。在2年能检测到的出仁率QTL中,LG02上的区间AHGS0344~AGGS2438有与荚果长相关的QTL。在1年能检测到的出仁率QTL中,LG13上的区间AHTE0470~AGGS1233有与荚果长、百果重相关的QTL,LG06上的区间AGGS1363~AHGA24894有与荚果长相关的QTL,LG18上的区间AHTE0381~AGGS0100有与荚果宽、荚果厚相关的QTL。

关键词: 花生, 出仁率, QTL, 相关分析

Abstract:

Peanut is an important oil crops, the shelling percentage of mature pods in peanut is not only associated with peanut oil, also related to the thickness of shell and the extent of easy-shelling. Therefore the shelling percentage is a crucial component for peanut genetic breeding. In this study, 188 recombinant inbred lines (RIL), derived from a cross between two Spanish type peanut cultivars (Yuanza 9102 × Xuzhou 68-4) were used to analyze their in 2013 and 2014 years phenotype data and shelling percentage. Compared with their parents, 29 lines had higher shelling percentage. There were significantly or very significantly negative correlation between shelling percentage and pod size related traits. On the basis of a linkage map (containing 365 markers and 22 linkage groups) constructed before, QTL mapping of shelling percentage traits was conducted by using CIM model in WinQTLcart 2.5. A total of 22 QTLs were detected in the two environments, every single QTL explained the phenotypic variation ranging from 3.61% to 13.49%. A total of five intervals (AHGS0344–AGGS2438, AGGS0957–AHGA7048, AGGS0058–AHGA72558, AHTE0446–AHGA363492, AGGS0311–AGGS2287) were detected in both environments to be located on the linkage group 2, linkage group 3 and linkage group 10 explaining phenotypic variation of 3.68%–13.99%. There were QTLs both related to pod and shelling percentage in four intervals, including AHGS0344–AGGS2438 on linkage group 2 and AGGS1363–AHGA24894 on linkage group 6 containing QTLs related to pod length; AHTE0470–AGGS1233 on linkage group 13 containing QTLs related to pod length and weight of 100-pod; AHTE0381–AGGS0100 on linkage group 18 containing QTLs related to pod, besides genetic effects between shelling percentage and pod traits in the same intervals were opposite.

Key words: Cultivated peanut, Shelling percentage, QTL, Correction analysis

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