Welcome to Acta Agronomica Sinica,

Acta Agron Sin ›› 2007, Vol. 33 ›› Issue (04): 646-651.

• ORIGINAL PAPERS • Previous Articles     Next Articles

Application of Electronic Probe on Element Content Measurement in Plant Leaves and Microzone Analysis

LI Mao-Teng**,FU Chun-Hua**,LIU Jian-Min,LI Qiang,LI Wei,LI Tao,YU Long-Jiang*   

  1. College of Life Science and Technology, Huazhong Unversity of Science and Technology, Wuhan 430074, Hubei, China
  • Received:2006-06-13 Revised:1900-01-01 Online:2007-04-12 Published:2007-04-12

Abstract:

The element analysis is most important in agriculture production. There are many methods for analyzing the element content, which are complicated in sample-treatment and cannot be used for analyzing the element content in the leaf surface microzones. The electronic probe method one with the merits of simple pre-treatment without sample destruction and the analyses of the element content and their distribution in microzones which made it is widely used in metallurgical, geological, archeological fields. In this paper, we analyzed the element composition and contents in soil and mesophyll cells of four species of plant by using electronic probe. The results showed that the element composition and contents in mesophyll cells could be well measured through this method. The elemental contents in mesophyll cells were not certainly correlated with those in the soil, which indicated that the elements absorption of plants was determined by its heredity and also affected by the element content of the soil. The linear scanning method can be show the element content in every scanning point along the restricted line, the results indicated that the element content was varied a lot in the different scanning points of leaves. The map scanning technique also could demonstrate the element content and their distribution in a particular region, for example, in the particle structure of leaf surface of B. napus. In conclusion, the electronic probe technology can be effectively used in analyzing element content in microzone of plant leaf surface.

Key words: Electronic probe, Element pontent, Microzone analysis

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