Abstract: Wheat (Triticum aestivum L.) breeding line Tm3314 is a T-type non-1B/1R material carrying 1BS of T. macha. KTm3314A, a K-type non-1B/1R male sterile line developed from Tm3314, is easily restored in fertility, does not produce haploids in reproduction, and has high temperature-sensitivity, thus has potential in wheat hybrid breeding. The T-type restorer gene Rf3, which is closely linked to K-type male sterile gene rfv1 on 1BS in T. spelta, is used as a marker of rfk1 under the background of T. timophvee cytoplasm. However, some male sterile materials with substitutive chromosome fragments of the 1BS of T. macha have been found the absence of rfv1 under T. timophvee cytoplasm background. To understand the linkage relationship between Rf3 and rfv1 in T. macha, the segregation generations of (T504A/Tm3314) F₂ and T504A//KTm3314A/90(13)21 as well as their backcross populations with a K-type sterile line K119A were used in field experiments in 2003–2006. The results showed that crossing-over value between Rf3 and rfv1 was 16.54%.

Key words: Triticum macha L., Major T-type restorer gene Rf3, Major K-type male sterile gene rfv1