Welcome to Acta Agronomica Sinica，

Acta Agron Sin ›› 2009, Vol. 35 ›› Issue (7): 1374-1378.doi: 10.3724/SP.J.1006.2009.01374

• RESEARCH ACTIVITIES • Previous Articles    

Polymorphism Analysis of Seedling Storage Proteins in Foxtail Millet（Setaria italic L.Beauv.）with A-PAGE

YANG Yan-Bing,ZHANG Hua-Wen,QIN Ling,WANG Hai-Lian,GUAN Yan-An*,DUAN Nai-Bin,ZHANG Han   

  1. Crop Research Institute,Shandong Academy of Agricultural Science,Jinan250100,China
  • Received:2008-12-05 Revised:2009-03-20 Online:2009-07-12 Published:2009-05-19
  • Contact: GUAN Yan-An, E-mail: yguan65@yahoo.com.cn

PDF

2027

Cited

5

Abstract:

The polymorphism of water-soluble protein, salt-soluble protein, acid-soluble protein and prolamin was analyzed by A-PAGE in 24 foxtail millet varieties from different ecological zones. The results revealed that the banding patterns of the water-soluble protein in different millet varieties were similar to those of the acid-soluble protein. There existed over twenty bands of the salt-soluble protein in each millet variety, and only four varieties contained polymorphic bands, showing that bands of the water-soluble protein, the salt-soluble protein and the acid-soluble protein obtained by A-PAGE are not available to use in millet varieties identification. There existed a certain degree of heterogeneity in millet prolamin bands. Seven to ten prolamin bands appeared in tested varieties, five of which were the common ones, two to five of which were polymorphic, so prolamin patterns of millet varieties can be used in variety identification and evaluation. The results showed that there was less variance for proteins in tested millet varieties from different ecological zones compared with other major cereal crops like wheat and corn, which implies that only a limited heterogeneity in the genetic background of millet varieties. Therefore, it is very important to enrich the genetic basis of millet genetic stocks to develop elite varieties.

Key words: Foxtail millet(Setaria italica L.Beauv.), Seed storage protein, Prtein polymorphism, A-PAGE


[1] Yang T-Y(杨天育), Huang X-G(黄相国), He J-H(何继红), Shen Y-H(沈裕琥), Wu G-Z(吴国忠). Advance in diversity of genetic resource of foxtail millet. Acta Agric Boreali-Occident Sin (西北农业学报), 2003, 12(1): 43-47(in Chinese with English abstract)


[2] Metakovsky E V, Branlard G. Genetic diversity of French wheat germplasm base on gliadin alleles. Theor Appl Genet,1998, 96: 209-218


[3] Liu H(刘华), Jia J-Z(贾继增). Fingerprint application in crop variety identification. Crop Genet Resour (作物品种资源), 1997, (2): 45-48(in Chinese)


[4] Zhang F(张芳), Chen J-T(陈景堂), Zhu L-Y(祝丽英), Song Z-Q(宋占权), Huang Y-Q(黄亚群), Liu Z-Z(刘志增), Chi S-M(池书敏). Preliminary studies on identification of sweet corn hybrids with technique of polyacrylamide gel electrophoresis of albumins. Chin Agric Sci Bull (中国农学通报), 2005, 21(8): 46-48 (in Chinese with English abstract)


[5] Song T-M(宋同明), Zheng D-J(郑大浩), Liu Y(刘岩). Identification of corn varieties using lactate polyacrylamide gel electrophoresis of seed albumins and globulins. Acta Bot Sin (植物学报), 1996, 38(8): 599-604(in Chinese with English abstract)


[6] Du Y-H(杜月红), Yu Y-T(于永涛), Shi Y-S(石云素), Song Y-C(宋燕春), Li Y(黎裕), Wang T-Y(王天宇). Genetic diversity of seed storage proteins in maize germplasm by acid polyacrylamide gel electrophoresis (A-PAGE). J Plant Genet Resour (植物遗传资源学报), 2007, 8(4): 430-435 (in Chinese with English abstract)


[7] Niu Y-Z(牛应泽), Liu R-S(刘镕山), Li F-A(李方安). Identification of maize varieties by seed protein electrophoresis: Comparison of seed zeins and salt soluble protein.J Sichuan Agric Univ (四川农业大学学报), 1995, 13(2): 150-153(in Chinese with English abstract)


[8] Yang T-Y(杨天育), Shen Y-H(沈裕琥), Huang X-G(黄相国), He J-H(何继红), Wu G-Z(吴国忠). Identification of genetic diversity in the foxtail millet (Setaria italic L. Beauv.) by A-PAGE. Acta Agron Sin (作物学报), 2005, 31(1): 131-133(in Chinese with English abstract)


[9] Kumar K K, Parvathy P K. Characterization of storage protein from selection varieties of foxtail millet

[Setaria italica (L.) Beauv.]. J Sci Food Agric, 1998, 77: 535-542


[10] Montoiro V P, Virupaksha T K, Rajagopol R D. Proteins of Italian millet: Amino acid composition, solubility fractionation and electrophoresis of protein fraction. J Sci Food Agric, 1982, 33: 1072-1079


[11] Li Y(黎裕), Wang Y-R(王雅儒). Varibility for seed protein in foxtail millet and relative varieties. Crop Genet Resour (作物品种资源),1998, (2): 10-12(in Chinese)


[12] Zhang H-W(张华文), Yang Y-B(杨延兵), Qin L(秦岭), Guan Y-A(管延安), Duan N-B(段乃彬), Ni D-P(倪大鹏), Yu D-A(于东安). Optimization of A-PAGE for isolation foxtail millet (Setaria italica L.Beauv) salt-soluble proteins. Shandong Agric Sci (山东农业科学), 2007, (5): 34-37(in Chinese with English abstract)


[13] Zhang C-Q(张春庆), Yin Y-P(尹燕枰). Theories and Technologies of Seed Quality Test (种子质量检验的理论与技术). Beijing: Chinese Agriculture Science and Technology Press, 1995. pp 105-120(in Chinese)


[14] Zhang Y-B(张延滨), Xin W-L(辛文利), Sun L-F(孙连发), Xiao Z-M(肖志敏), Qi S-Y(祁适雨). Flour quality differences between near-isogenic lines with HMW glutenin subunits 2+12 and 5+10 in wheat. Acta Agron Sin (作物学报), 2003, 29(1): 93-96 (in Chinese with English abstract)


[15] Yang Y-B(杨延兵), Gao R-Q(高荣岐), Yin Y-P(尹燕枰), Guan Y-A(管延安). Dynamic changes of free amino acid, protein and its components in wheat with different quality traits. J Triticeae Crops (麦类作物学报), 2007, 27(5): 864-86(in Chinese with English abstract)


[16] Yang Y-B(杨延兵), Guan Y-A(管延安), Zhang H-W(张华文), Xu P-P(徐平平), Zhang W-L(张文兰), Chen L-R(陈利容), Qin L(秦岭). Genetic variation among varieties of foxtail millet (Setaria italica L. Beauv.) based on RAPD markers. Acta Agric Boreal-Sin (华北农学报), 2007, 22(4): 134-136 (in Chinese with English abstract)


[17] Yang T-Y(杨天育), Dou Q-W(窦全文), Shen Y-H(沈裕琥), Huang X-G(黄相国), He J-H(何继红), Wu G-Z(吴国忠). Genetic variation among varieties of foxtail millet from different regions in China based on RAPD markers. Acta Bot Boreal-Occident Sin (西北植物学报), 2003, 23(5): 765-770 (in Chinese with English abstract)
[1] ZHOU Li-Hui, LIU Qiao-Quan, ZHANG Chang-Quan, XU Yong, TANG Su-Zhu, et al.. Variation and Distribution of Seed Storage Protein Content and Composition among Different Rice Varieties [J]. Acta Agron Sin, 2009, 35(5): 884-891.
[2] ZHOU Li-Hui,LIU Qiao-Quan,GU Ming-Hong*. Milling Characteristics and Distribution of Seed Storage Proteins in Rice with Various Grain shapes [J]. Acta Agron Sin, 2009, 35(2): 317-323.
[3] Liu Hua;Wang Yusheng;Zhang Hui;Cao Yongsheng;Zhou Ronghua;Jia Jizeng. Preliminary Construction and Application of GIiadin Fingerprints Database of Chinese Wheat Germplasm [J]. Acta Agron Sin, 1999, 25(06): 674-682.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Add: No. 80 Xueyuan South Rd, Beijing 100081, P. R. China E-mail: zwxb301@caas.cn
Supported by Beijing Magtech Co.Ltd