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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1148-1154.doi: 10.3724/SP.J.1006.2012.01148

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Polymorphism of Starch Granule-Associated Proteins and 5′ Leader Sequence of GBSSI Gene in Indigenous Naked Barley (Hordeum vulgare L.) from Qinghai-Tibetan Plateau in China

WANG Chun-Ping1,2,**,GAO Li-Juan1,2,**,PAN Zhi-Fen1,*,NIMA Zha-Xi3,TANG Ya-Wei3,ZENG Xing-Quan3,LI Qiao1,2,DENG Guang-Bing1,2,LONG Hai1,YU Mao-Qun1,*   

  1. 1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China; 3 Tibet Academy of Agriculture and Animal Sciences / Key Laboratory of Biology and Genetic Breeding of Tibetan Naked Barley, Ministry of Agriculture, Lhasa 850032, China
  • Received:2011-12-05 Revised:2012-04-16 Online:2012-07-12 Published:2012-05-11
  • Contact: 潘志芬, E-mail: panzf@cib.ac.cn, Tel: 028-85226136; 余懋群, E-mail: yumq@cib.ac.cn, Tel: 028-85229053 E-mail:wcp49930000@sina.com

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Abstract: Starch granule-associated proteins (SGAPs) are minor components tightly bound with starch granules, most of which are believed to be starch biosynthetic enzymes. We separated SGAPs from 261 indigenous naked barley accessions in Qinghai-Tibetan Plateau of China using 1D-SDS-PAGE technique. A total of 20 polymorphic banding patterns with molecular weights of 45–100 kD and 78 SGAPs patterns were detected, of which bands 2, 3, 5, 10, and 11 were novel protein bands. The 5′ leader sequences of GBSSI gene were cloned using 236 accessions of indigenous naked barley. Two types of length polymorphisms (1 000 bp and 800 bp) were found with frequencies of 80.1% and 19.9%, respectively. According to sequencing results, there were 32 polymorphic sites in the 5′ leader sequences of GBSSI gene, including nine InDels and 23 SNPs. Specific mutations were found in Qinghai-Tibetan Plateau accessions. For example, the 400 bp deletion reported corresponding to low amylose content in barley was absent in this study; and special SNPs were detected in accessions yf127, yf70, 011Z1396, and 09Z586. These results imply that the SGAPs of the Qinghai-Tibetan Plateau indigenous naked barley are abundant and polymorphic. The novel sequence characteristics in the GBSSI5′ leader sequence of the low amylose accessions suggest a different mechanism for amylose biosynthesis in Qinghai-Tibetan Plateau indigenous naked barley.

Key words: Indigenous variety, Naked barley, Starch granule-associated proteins (SGAPs), GBSSI gene

[1]Paul M B. Starch granule-associated proteins and polypeptides: a review. Starch, 2001, 53: 475–503

[2]Wang C P, Pan Z F, Nima Z X, Tang Y W, Cai P, Liang J J, Deng G B, Long H, Yu M Q. Starch granule-associated proteins of hull-less barley (Hordeum vulgare L.) from the Qinghai-Tibet Plateau in China. J Sci Food Agric, 2011, 91: 616–624

[3]Pan Z-F(潘志芬), Zou Y-X(邹奕星), Zhao T(赵桃), Deng G-B(邓光兵), Zhai Z-G(翟旭光), Wu F(吴芳), Yu M-Q(余懋群). SGP polymorphism in cultivated naked barley from Qinghai-Tibet Plateau in China and the relationship between SGPs and starch content. Hereditas (遗传), 2007, 29(5): 599–606 (in Chinese with English abstract)

[4]Ge H F, Wang Z Y, Hong M M. A 31 bp fragment within the 5′upstream region of rice waxy gene enhances gene expression. Acta Phytophysiol Sin, 2000, 26: 159–163

[5]Van de Wal M H B J, Jacobsen E, Visser R G F. Multiple allelism as a control mechanism in metabolic pathways: GBSSI allelic composition affects the activity of granule-bound starch synthase I and starch composition in potato. Mol Genet Genomics, 2001, 265: 1011–1021

[6]Zhu C-M(朱彩梅), Zhang J(张京). Single nucleotide polymorphism of Wx gene associated with amylose content in barley germplasm. Sci Agric Sin (中国农业科学), 2010, 43(5): 889–898 (in Chinese with English abstract)

[7]Wu K-L(吴昆仑), Zhao Y(赵媛), Chi D-Z(迟德钊). Relationship between polymorphism of Wx gene and amylose content in hull-less barley. Acta Agron Sin (作物学报), 2012, 38(1): 71–79 (in Chinese with English abstract)

[8]Domon E, Fuijita M, Ishikawa N. The insertion/deletion polymorphisms in the waxy gene of barley genetic resources from East Asia. Theor Appl Genet, 2002, 104: 132–138

[9]Patron N J, Smith A M, Fahy B F, Hylton C M, Naldrett M J, Rossnagel M J, and Denyer K. The altered pattern of amylose accumulation in the endosperm of low-amylose barley cultivars is attributable to a single mutant allele of granule-bound starch synthase I with a deletion in 5,-non-coding region. Plant Physiol, 2002, 130: 190–198

[10]Pan Z-F(潘志芬), Deng G-B(邓光兵), Yu M-Q(余懋群). An improved 1D-SDS-PAGE method for identification of wheat waxy protein. Chin J Appl Environ Biol (环境与应用生物学报), 2000, 6(5): 487–489 (in Chinese with English abstract)

[11]Wang G-L(王关林), Fang H-J(方宏筠). Theory of Plant Genetic Engineering (植物基因工程原理), 2nd Edn. Beijing: Science Press, 2005 (in Chinese)

[12]Peng M, Gao M, Båga M, Hucl P, Chibbar R N. Starch-branching enzymes preferentially associated with A-type starch granules in wheat endosperm. Plant Physiol, 2000, 124: 265–272

[13]Borén M, Larsson H, Falk A, Jansson C. The barley starch granule proteome-internalized granule polypeptides of the mature endosperm. Plant Sci, 2004, 166: 617–626

[14]Regina A, Kosar-Hashemi B, Li Z, Pedler A, Mukai Y, Yamamoto M, Gale K, Sharp P J, Morell M K, Rahman S. Starch branching enzyme IIb in wheat is expressed at low levels in the endosperm compared to other cereals and encoded at a non-synthetic locus. Planta, 2005, 222: 899–909

[15]Alexander R D, Morris P C. A proteomic analysis of 14-3-3 binding proteins from developing barley grains. Proteomics, 2006, 6: 1886–1896

[16]Hennen-Bierwagen T A, Lin Q H, Grimaud F, Planchot V, Peter L K, Martha G J, Alan M M. Proteins from multiple metabolic pathways associate with starch biosynthetic enzymes in high molecular weight complexes: a model for regulation of carbon allocation in maize amyloplasts. Plant Physiol, 2009, 149: 1541–1559

[17]Miural H, Sugawara A. Effects of the three Wx genes on amylose synthesis in wheat endosperm. Theor Appl Genet, 1996, 93: 1066–1070
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