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Acta Agron Sin ›› 2012, Vol. 38 ›› Issue (07): 1148-1154.doi: 10.3724/SP.J.1006.2012.01148

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Polymorphism of Starch Granule-Associated Proteins and 5′ Leader Sequence of GBSSI Gene in Indigenous Naked Barley (Hordeum vulgare L.) from Qinghai-Tibetan Plateau in China

WANG Chun-Ping1,2,**,GAO Li-Juan1,2,**,PAN Zhi-Fen1,*,NIMA Zha-Xi3,TANG Ya-Wei3,ZENG Xing-Quan3,LI Qiao1,2,DENG Guang-Bing1,2,LONG Hai1,YU Mao-Qun1,*   

  1. 1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China; 2 Graduate School of Chinese Academy of Sciences, Beijing 100039, China; 3 Tibet Academy of Agriculture and Animal Sciences / Key Laboratory of Biology and Genetic Breeding of Tibetan Naked Barley, Ministry of Agriculture, Lhasa 850032, China
  • Received:2011-12-05 Revised:2012-04-16 Online:2012-07-12 Published:2012-05-11
  • Contact: 潘志芬, E-mail: panzf@cib.ac.cn, Tel: 028-85226136; 余懋群, E-mail: yumq@cib.ac.cn, Tel: 028-85229053 E-mail:wcp49930000@sina.com

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Abstract: Starch granule-associated proteins (SGAPs) are minor components tightly bound with starch granules, most of which are believed to be starch biosynthetic enzymes. We separated SGAPs from 261 indigenous naked barley accessions in Qinghai-Tibetan Plateau of China using 1D-SDS-PAGE technique. A total of 20 polymorphic banding patterns with molecular weights of 45–100 kD and 78 SGAPs patterns were detected, of which bands 2, 3, 5, 10, and 11 were novel protein bands. The 5′ leader sequences of GBSSI gene were cloned using 236 accessions of indigenous naked barley. Two types of length polymorphisms (1 000 bp and 800 bp) were found with frequencies of 80.1% and 19.9%, respectively. According to sequencing results, there were 32 polymorphic sites in the 5′ leader sequences of GBSSI gene, including nine InDels and 23 SNPs. Specific mutations were found in Qinghai-Tibetan Plateau accessions. For example, the 400 bp deletion reported corresponding to low amylose content in barley was absent in this study; and special SNPs were detected in accessions yf127, yf70, 011Z1396, and 09Z586. These results imply that the SGAPs of the Qinghai-Tibetan Plateau indigenous naked barley are abundant and polymorphic. The novel sequence characteristics in the GBSSI5′ leader sequence of the low amylose accessions suggest a different mechanism for amylose biosynthesis in Qinghai-Tibetan Plateau indigenous naked barley.

Key words: Indigenous variety, Naked barley, Starch granule-associated proteins (SGAPs), GBSSI gene

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