Abstract:

Stylosanthes guianensis is regarded as one of the newest green leguminous forage in tropical and subtropical regions of China. It is a species of stylosanthes (Stylosanthes spp.), which has good traits including high dry matter yield, cold tolerance, anthracnose resistance and late maturity. But there were few reports on optimal media recipes for tissue culture in Stylosanthes guianensis. To investigate the main factors affecting tissue culture, callus was induced with a cultivar Reyan 10 (Stylosanthes guianensis cv.), surviving callus was cultured on different media after 2 weeks, then, the buds with 2–3 cm in length were regenerated on root induction medium after 3 weeks, finally they were transplanted into soil as the roots had formed after 2–3 weeks. Meanwhile the main medium components affecting the tissue culture were determined with orthogonal design, and mathematical model between the medium components and traits was established. The results was showed that there was a real regression relation between regulated factors and rate of callus induction, coefficient of bud differentiation, rate of root induction, with the equations of Y = 86.90+3.34X1+1.36X3+2.86X1X3, Y=7.70+1.52X2 and Y=299.30 +6.39X1+4.32X1X2 respectively. For callus induction, NAA was the most efficient factor, interaction between NAA and sucrose was the second factor, and sucrose was the last one. Also, the important factors were KT for bud induction as well as NAA and NAA/IAA for root induction.

Key words: Stylosanthes, Cotyledon culture, Multifactor Combination, Mathematical model, Orthogonal design