Abstract:

To investigate the mechanism with which the herbicides works, chloroplast proteome was analyzed in Chinese cabbage seedlings treated with PSⅡinhibiting herbicide Atrazine by using 2-DE techniques and MALDI-TOF MS. The results indicated that 23 protein spots showed significant and reproducible changes in treated-seedlings, of which 4 protein spots were down–regulated (spots 15-18) and 5 protein spots up-regulated (spots 19-23). Fourteen protein spots showed qualitative changes between control and treated samples. Ten out of 14 proteins (spots 5-14) were newly synthesized in the treated seedlings. Four out of 14 protein spots (spots 1-4) disappeared treated with high concentration Atrazine (10 mg L^-1), and not with low concentration Atrazine (0.01, 0.1, and 1 mg L^-1, respectively). From the 14 protein spots, spots 1, 2, 5, 6, 8, 10, and 13 were respectively identified as glycos-transf-Ⅰ, isoprene synthase, hypothetic protein, proline dehydrogenase, putative 6-phospho-fructo kinase isozymeⅡ, calcium transporting ATPase, and maturase K, respectively. Changes in chlorophyll content and soluble protein content were also determined in treated seedlings. It was found that chlorophyll a content decreased, while chlorophyll b content showed a little increase without significant difference. The soluble protein content increased with increasing treatment concentration.

Key words:

PSⅡinhibiting herbicide Atrazine, Chloroplast proteome, Qualitative change, MALDI-TOF MS, Chinese cabbage