欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1366-1376.doi: 10.3724/SP.J.1006.2013.01366

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

从携带S5n基因的水稻种质中发掘San、Sbn和Scn基因

李宏岩**,王思哲**,SHAHID Muhammad Qasim**,陈志雄,王兰,陈凤宜,刘向东*,卢永根*   

  1. 华南农业大学 / 亚热带农业生物资源保护与利用国家重点实验室, 广东广州 510642
  • 收稿日期:2012-11-30 修回日期:2013-04-22 出版日期:2013-08-12 网络出版日期:2013-05-20
  • 通讯作者: 刘向东, E-mail: xdliu@scau.edu.cn; 卢永根, yglu@scau.edu.cn
  • 基金资助:

    本研究由国家自然科学基金项目(30971756)和教育部高等学校博士学科点专项科研基金项目资助。

Excavation of Neutral Alleles San, Sbn, and Scn from the Rice Germplasm Harboring S5n Gene

LI Hong-Yan**,WANG Si-Zhe**,SHAHID Muhammad Qasim**,CHEN Zhi-Xiong,WANG Lan,CHEN Feng-Yi,LIU Xiang-Dong*,LU Yong-Gen*   

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou 510642, China?
  • Received:2012-11-30 Revised:2013-04-22 Published:2013-08-12 Published online:2013-05-20
  • Contact: 刘向东, E-mail: xdliu@scau.edu.cn; 卢永根, yglu@scau.edu.cn

RichHTML

PDF

1017

摘要：

花粉不育是籼粳杂种F1优势利用的主要障碍之一。包括Sa、Sb和Sc等至少6个基因座位内的等位基因互作会引起花粉不育，这些座位上的中性等位基因可以克服不育性。所以，发掘和利用中性等位基因具有重要意义。本文用携带S5n的水稻种质，分别与台中65及其携带花粉不育基因的一套近等基因系杂交，组配具有单个座位互作和多个座位同时互作的杂种F1，首先通过观察杂种F1的花粉育性并比较相应杂种F1育性的差异，初步判断是否具有中性等位基因，然后，采用与Sa、Sb和Sc座位紧密连锁的分子标记对F2植株基因型的分离进行检测，并分析其分离比例的符合度，确定存在中性等位基因的真实性。结果发现在所鉴定的6份材料中有2份(灰背子和Madhukar)同时携带San和Sbn，3份(饭毫皮、秕五升和粤泰B)携带Sbn，1份(Jackson)携带Scn。这些材料同时携带可克服杂种F1胚囊不育和花粉不育的基因，是克服籼粳杂种F1不育性的重要基因来源。

关键词: 水稻(Oryza sativa L.), 等位基因互作, 杂种不育性, 雄性不育, S5n

Abstract:

One of the principal barriers in utilization of heterosis between indica and japonica subspecies of Asian cultivated rice is F1 pollen sterility. According to the “specific compatibility gene” theory, there are at least six loci, which cause indica and japonica hybrid pollen sterility. Excavation and utilization of neutral alleles for overcoming F1 hybrid sterility have great importance in hybrid breeding. Varieties carrying S5n gene were used to cross with Taichung 65 and its near isogenic lines (NILs) to produce F1 hybrids with single-locus interaction, double-loci interaction and triple-loci interaction. Pollen fertility and seed setting rate of these hybrids were observed in F1 to pre-judge the existence of neutral alleles and then F2generation was genotyped with molecular markers tightly linked to Sa, Sb and Scloci to verify the presence of San, Sbn and Scn by detecting segregation ratio. The results showed that two materials (Huibeizi and Madhukar) carried San and Sbn, three materials (Fanhaopi, Biwusheng and YuetaiB) carried Sbn and one material (Jackson) carried Scn. These materials have wide compatibility and neutral alleles are important germplasm for hybrid breeding and knowing the molecular mechanisms underlying hybrid male sterility.

Key words: Rice (Oryza sativa L.), Allelic interaction, Hybrid sterility, Male sterility, Wide compatibility gene (S5n)

[1]Ji Q, Zhang M J, Lu J F, Wang H M, Lin B, Liu Q Q, Chao Q, Zhang Y, Liu C X, Gu M H, Xu M L. Molecular basis underlying the S5-dependent reproductive isolation and compatibility of indica/japonica rice hybrids. Plant Physiol, 2012, 158: 1319–1328



[2]Zeng Y X, Hu C Y, Lu Y G, Li J Q, Liu X D. Diversity of abnormal embryo sacs in indica/japonica hybrids in rice demonstrated by confocal microscopy of ovaries. Plant Breed, 2007, 126: 574–580



[3]Zeng Y X, Hu C Y, Lu Y G, Li J Q, Liu X D. Abnormalities occurring during female gametophyte development result in the diversity of abnormal embryo sac and leads to abnormal fertilization in indical/japonica hybrids in rice. J Integr Plant Biol, 2009, 51: 3–12



[4]Takahiko K, Atsushi Y , Nori K. Hybrid male sterility in rice is due to epistatic interactions with a pollen killer locus. Genetics, 2011, 189: 1083–1092



[5]Chen J J, Ding J H, Ou-Yang Y D, Du H Y, Yang J Y, Cheng K, Zhao J, Qiu S Q, Zhang X L, Yao J L, Liu K D, Wang L, Xu C G, Li X H, Xue Y B, Xia M, Ji Q, Lu J F, Xu M L, Zhang Q F. A triallelic system of S5 is a major regulator of the reproductive barrier and compatibility of indica-japonica hybrids in rice. Proc Natl Acad Sci USA, 2008, 105:11436–11441



[6]Yang Y X, Wu J W, Chen Z X, Wang L, Li J Q, Liu X D, Lu Y G. Mining rice new germ plasm containing S5n gene by functional molecular marker and sequencing. Chin Sci Bull, 2009, 54: 3258–3264



[7]Yang Y X, Li Y H, Tong J F, Shahid M Q, Chen Z X, Wang L, Li J Q, Liu X D, Lu Y G. Wide-compatibility gene S5n exploited by functional molecular markers and its effect on fertility of intersubspecific rice hybrids. Crop Sci, 2012, 52: 669–675



[8]Wei C M, Wang L, Yang Y X, Shahid M Q, Li J Q, Liu X D, Lu Y G. Identification of an S5n allele in Oryza rufipogon Griff. and its effect on embryo sac fertility. Chin Sci Bull, 2010, 55: 1255–1262



[9]Tong J F, Li Y H, Yang Y X, Shahid M Q, Chen Z X, Wang L, Li J Q, Liu X D, Lu Y G. Molecular evolution of rice S5n and functional comparison among different sequences. Chin Sci Bull, 2011, 56: 2016–2024



[10]Long Y M, Zhao L F, Niu B X, Su J, Wu H, Chen Y L, Zhang Q Y, Guo J X, Zhuang C X, Mei M T, Xia J X, Wang L, Wu H B, Liu Y G. Hybrid male sterility in rice controlled by interaction between divergent alleles of two adjacent genes. Proc Natl Acad Sci USA, 2008, 105: 18871–18876



[11]Zhang G-Q(张桂权), Lu Y-G(卢永根). Genetic studies on the hybrid sterility in cultivated rice(Oryza sativa): I. Diallel analysis of the hybrid sterility among isogenic F1 sterile lines. Chin J Rice Sci(中国水稻科学), 1989, 3(3): 97–101(in Chinese with English abstract)



[12]Zhang G-Q(张桂权), Lu Y-G(卢永根). Genetic studies of the hybrid sterility in cultivated rice(Oryza sativa): II. A genic model for F1 pollen sterility. Acta Genet Sin (遗传学报), 1993, 20(3): 222–228 (in Chinese with English abstract)



[13]Zhang G-Q(张桂权), Lu Y-G(卢永根), Zhang H(张华), Yang J-C(杨进昌), Liu G-F(刘桂富). Genetic studies on the hybird sterility in culitivated rice (Oryza sativa): IV. Genotypes for F1 pollen sterillity. Acta Genet Sin (遗传学报), 1994, 21(1): 34–41 (in Chinese with English abstract)



[14]Ding X-H(丁效华), Zhang Z-M(张泽民), Zeng R-Z(曾瑞珍), Li W-T(李文涛), Zhang G-Q(张桂权). Genotypic identification of the S-b locus in the indica-compatible japonica lines of rice (Oryza sativa). Chin J Rice Sci (中国水稻科学), 2003, 17(4): 297–300 (in Chinese with English abstract)



[15]Ding X-H(丁效华), Zhang Z-M(张泽民), Zeng R-Z(曾瑞珍), Zhang G-Q(张桂权). Genotypic identification of the Sc locus in the indica-compatible japonica lines of rice (Oryza sativa L.). Acta Agric Boreali-Sin (华北农学报), 2007, 22(4): 5-8 (in Chinese with English abstract)



[16]Shi L G, Liu X D, Liu B, Zhao X J, Wang L, Li J Q, Lu Y G. Identifying neutral allele Sb at pollen-sterility loci in cultivated rice with Oryza rufipogon origin. Chin Sci Bull, 2009, 54: 3813–3821



[17]Wang Y, Zhong Z Z, Zhao Z G, Jiang L, Bian X F, Zhang W W, Liu L L, Ikehashi H, Wan J M. Fine mapping of a gene causing hybrid pollen sterility between Yunnan weedy rice and cultivated rice (Oryza sativa L.) and phylogenetic analysis of Yunnan weedy rice. Planta, 2010, 231: 559–570



[18]Li J Q, Shahid M Q, Feng J H, Liu X D, Zhao X J, Lu Y G. Identi?cation of neutral alleles at pollen sterility gene loci of cultivated rice (Oryza sativa L.) from wild rice (O. ru?pogon Griff.). Plant Syst Evol, 2012, 298:33–42



[19]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sativa L). Mol Gen Genet, 1996, 252: 597–607



[20]Huang C-F(黄朝峰), Zhang G-Q(张桂权). Development of position-specific microsatellite markers and molecular mapping of insect resistant genes in rice (Oryza sativa L.). Mol Plant Breed (分子植物育种), 2003, 11(4): 572–574 (in Chinese with English abstract)



[21]Shahid M Q, Chen F Y, Li H Y, Wang S Z, Chen P F, Lin S Q, Liu X D and Lu Y G. Double-neutral genes, Sa-n and Sb-n , for pollen fertility in rice to overcome indica × japonica hybrid sterility.Crop Sci, 2013, 53:164–176



[22]Li W T, Zeng R Z, Zhang Z M, Ding X H, Zhang G Q. Fine mapping of F1 pollen sterility locus S-b in rice(Oryza sativa L.). Chin Sci Bull, 2006, 51: 675–680



[23]Yang C Y, Chen Z Z, Zhuang C X, Mei M T, Liu Y G. Genetic and physical fine-mapping of the Sc locus conferring indica-japonica hybrid sterility in rice (Oryza sativa L.). Chin Sci Bull, 2004, 49: 1718–1721



[24]Li W T, Zeng R Z, Zhang Z M, Ding X H, Zhang G Q. Identification and fine mapping of S-d , a new locus conferring the partial pollen sterility of intersubspecific F1 hybrids in rice(Oryza sativa L.). Theor Appl Genet, 2008, 116: 915–922



[25]Zhu W-Y(朱文银), Li W-T(李文涛), Ding X-H(丁效华), Zhang Z-M(张泽民), Zeng R-Z(曾瑞珍), Zhu H-T(朱海涛), Zhang G-Q(张桂权). Preliminary identification of F1 pollen sterility gene S-e in Oryza sativa. J South China Agric Univ (华南农业大学学报), 2008, 29(1): 1–5 (in Chinese with English abstract)



[26]Zhang Z S, Lu Y G, Liu X D, Feng J H, Zhang G Q. Cytological mechanism of pollen abortion resulting from allelic interaction of F1 pollen sterility locus in rice (Oryza sativa L.). Genetica, 2006, 7: 295–302
[1] 王娜, 白建芳, 马有志, 郭昊宇, 王永波, 陈兆波, 赵昌平, 张立平. 小麦lncRNA27195及其靶基因TaRTS克隆及表达分析[J]. 作物学报, 2021, 47(8): 1417-1426.
[2] 周步进, 李刚, 金刚, 周瑞阳, 刘冬梅, 汤丹峰, 廖小芳, 刘一丁, 赵艳红, 王颐宁. 利用红麻HcPDIL5-2a非全长基因创制雄性不育新种质[J]. 作物学报, 2021, 47(6): 1043-1053.
[3] 吴然然, 林云, 陈景斌, 薛晨晨, 袁星星, 闫强, 高营, 李灵慧, 张勤雪, 陈新. 绿豆雄性不育突变体msm2015-1的遗传学与细胞学分析[J]. 作物学报, 2021, 47(5): 860-868.
[4] 唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[5] 李京琳, 李佳林, 李新鹏, 安保光, 曾翔, 吴永忠, 黄培劲, 龙湍. 水稻ptc1隐性核不育系的创制及其配合力分析[J]. 作物学报, 2021, 47(11): 2173-2183.
[6] 蒋会兵,杨盛美,刘玉飞,田易萍,孙云南,陈林波,唐一春. 厚轴茶雄性不育株花药败育的生物学特性和细胞学研究[J]. 作物学报, 2020, 46(7): 1076-1086.
[7] 田士可, 秦心儿, 张文亮, 董雪, 代明球, 岳兵. 玉米雄性不育突变体mi-ms-3的遗传分析及分子鉴定[J]. 作物学报, 2020, 46(12): 1991-1996.
[8] 牟碧涛,赵卓凡,岳灵,李川,张钧,李章波,申汉,曹墨菊. 两份玉米CMS-C恢复系的育性恢复力测定及恢复基因的分子标记定位[J]. 作物学报, 2019, 45(2): 225-234.
[9] 王晓娟,潘振远,刘敏,刘忠祥,周玉乾,何海军,邱法展. 一个新的玉米silky1基因等位突变体的遗传分析与分子鉴定[J]. 作物学报, 2019, 45(11): 1649-1655.
[10] 孙辉**,张风廷**,王永波,叶志杰,秦志列,白秀成,杨吉芳,高建刚,赵昌平*. 雄性不育小麦BS210育性转换特性[J]. 作物学报, 2017, 43(02): 171-178.
[11] 陶瑶，王瑜，钟思荣，吴凌敏，谢丽娟，聂亚平，周玮，王建革，刘齐元. 烟草ATP合酶F0部分4个亚基基因转录本编辑位点分析[J]. 作物学报, 2016, 42(12): 1743-1753.
[12] 郭国强，孙学武，孙平勇，尹建英，何强，袁定阳，邓华凤，袁隆平. 水稻斑马叶突变体zebra1349的表型鉴定及基因精细定位[J]. 作物学报, 2016, 42(07): 957-965.
[13] 杨波,夏敏,张孝波,王晓雯,朱小燕,何沛龙,何光华,桑贤春*. 水稻早衰突变体esl6的鉴定与基因定位[J]. 作物学报, 2016, 42(07): 976-983.
[14] 张小军，岳福良，张小红，侯睿，张相琼，李文均. 利用宿根进行棉花不育系制种的技术体系研究[J]. 作物学报, 2015, 41(12): 1836-1843.
[15] 冯萍,邢亚迪,刘松,郭爽,朱美丹,娄启金,桑贤春,何光华,王楠. 水稻卷叶突变体rl28的特性与基因定位[J]. 作物学报, 2015, 41(08): 1164-1171.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2