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作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1377-1385.doi: 10.3724/SP.J.1006.2013.01377

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

山东省和河北省小麦白粉菌毒性与遗传多样性分析

赵紫慧1,2,黄江2,3,陆鸣1,王晓鸣2,吴龙飞2,武小菲2,赵鑫4,李洪杰2,*   

  1. 1 河北科技师范学院生命科技学院, 河北秦皇岛066600; 2 中国农业科学院作物科学研究所 / 农作物基因资源与基因改良国家重大科学工程,北京100081; 3 桂林医学院生物技术学院, 广西桂林541004; 4 西南大学植物保护学院, 重庆400700
  • 收稿日期:2013-01-07 修回日期:2013-04-22 出版日期:2013-08-12 网络出版日期:2013-05-20
  • 通讯作者: 李洪杰, E-mail: lihongjie@caas.cn
  • 基金资助:

    本研究由国家现代农业产业体系建设项目(CARS-3-1)，国家重点基础研究计划(973计划)项目(2009CB118300)，农业部作物种质资源保护子项目主要作物抗病虫、抗逆和品质性状鉴定评价项目(NB2012-2130135-25-15)资助。

Virulence and Genetic Diversity of Blumeria graminis f. sp. tritici Collected from Shandong and Hebei Provinces

ZHAO Zi-Hui1,3,HUANG Jiang2,3,LU Ming1,WANG Xiao-Ming2,WU Long-Fei2,WU Xiao-Fei2,ZHAO Xin4,LI Hong-Jie2,*   

  1. 1 College of Life Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China; 2 National Key Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 3 College of Biotechnology, Guilin Medical University, Guilin 541004, China; 4 College of Plant Protection, Southwest University, Chongqing 400700, China?
  • Received:2013-01-07 Revised:2013-04-22 Published:2013-08-12 Published online:2013-05-20
  • Contact: 李洪杰, E-mail: lihongjie@caas.cn

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摘要：

由Blumeria graminis f. sp. tritici引起的白粉病是危害我国小麦安全生产的重要病害之一。分析菌株毒性结构和抗病基因有效性对于利用寄主控制白粉病具有重要意义。本研究对2011年从山东和河北两省分离的41个菌株进行了毒性分析，并采用SSR标记对其遗传多样性进行了分析。测试菌株的毒性频率在0.35 (Bg40-2，山东烟台)至0.74 (Bg46-1，山东平度)之间。山东省菌株的平均毒性频率与河北省菌株没有显著差异。除别菌株外(例如Pm17)，山东省和河北省的菌株对大多数抗病基因的毒性差异不大。全部测试菌株对来自地方品种齿牙糙的Pm24基因都没有毒性。极少数菌株对Pm1c、Pm16、Pm20、PmH和Mlxbd的毒性频率低于0.1，在河北省邯郸市和黄骅市发现对Pm21基因具有毒性的菌株，但在山东省没有检测到对Pm21具有毒性的菌株。对Pm5e、 Pm6、 Pm12、 Pm13、 Pm17、 Pm40、 Pm2+6和Pm5+6的毒性频率在0.18~0.48之间，对Pm1a、 Pm3a、 Pm3c、P m3g、 Pm4a、 Pm4b、 Pm5a、 Pm7、 Pm8、 Pm19、 Pm33、 Pm43、 PmY39、 PmPS5A和Pm1+2+9的毒性频率超过0.6。遗传多样性分析可见，小麦白粉菌群体的遗传变异主要发生在群体内部，菌株间具有一定程度的基因交流。同一地点采集的不同单孢分离菌株有些可聚为一类，但有些不能聚为一类，说明其遗传基础可能存在差异。供试菌株对不同抗病基因的毒性多态性与DNA多态性之间不存在一一对应的关系。

关键词: 小麦, 白粉菌, 毒性, 抗病基因, 遗传多样性

Abstract:

Powdery mildew caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most important diseases that damages wheat (Triticum aestivum L.) production in China. Analysis of virulence structure and effectiveness of resistance genes is important in the control of powdery mildew with host resistance. This study was conducted to test virulence structures of 41 isolates collected from Shandong and Hebei provinces in 2011. The genetic diversity of these isolates was also analyzed using SSR markers. The virulence frequencies of isolates tested ranged from 0.35 (Bg40-2, Yantai, Shandong) to 0.74 (Bg46-1, Pingdu, Shandong). The mean virulence frequency for the isolates from Shandong was not significantly different from that of the isolates from Hebei Province. Except for a few resistance genes such as Pm17, the virulence frequencies of isolates from Shandong and Hebei provinces on most resistance genes were not different. None of the isolates tested was virulent on Pm24 carried by the Chinese landrace Chiyacao. A few isolates were virulent to Pm1c, Pm16, Pm20, PmH, and Mlxbd. Two isolates from Handan and Huanghuang of Hebei Province were virulent on Pm21, but none of the isolates from Shandong Province was virulent on Pm21. The virulence frequencies for Pm5e, Pm6, Pm12, Pm13, Pm17, Pm40, Pm2+6, and Pm5+6 ranged from 0.18 to 0.48. Genes Pm1a, Pm3a, Pm3c, Pm3g, Pm4a, Pm4b, Pm4c, Pm5a, Pm7, Pm8, Pm19, Pm33, Pm43, PmY39, PmPS5A, and PmDR147 had the virulence frequencies over 0.6. Analysis of genetic diversity indicated that the genetic variation of Bgt isolates occurred within the population, and gene flow occurred among isolates. Although some single spore progenies from the same location were clustered together, some were not classified into the same cluster, indicating the variation in their genetic bases. The polymorphism of virulence patterns for various resistance genes was not necessarily consistent with the DNA polymorphism as revealed by SSR markers.

Key words: Wheat, Blumeria graminis f. sp. tritici, Virulence, Resistance gene, Genetic diversity

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