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作物学报 ›› 2013, Vol. 39 ›› Issue (08): 1386-1390.doi: 10.3724/SP.J.1006.2013.01386

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

普通小麦中国春–簇毛麦易位系T1DL•1VS和T1DS•1VL的农艺和品质特性

董剑,杨华,赵万春*,李晓燕,陈其皎,高翔   

  1. 赵万春, E-mail:zhaowc2009@hotmail.com
  • 收稿日期:2012-12-14 修回日期:2014-01-11 出版日期:2013-08-12 网络出版日期:2013-04-23
  • 基金资助:

    本研究受到国家自然科学基金项目(31171538), 国家现代农业产业技术体系研究项目(CARS-3-2-47)和西北农林科技大学唐仲英育种基金(200902090015)资助。

Agronomic Traits and Grain Quality of Chinese Spring–Dasypyrum villosum Translocation Lines T1DL*1VS and T1DS*1VL

DONG Jian,YANG Hua,ZHAO Wan-Chun*,LI Xiao-Yan,CHEN Qi-Jiao,GAO Xiang   

  1. 赵万春, E-mail:zhaowc2009@hotmail.com
  • Received:2012-12-14 Revised:2014-01-11 Published:2013-08-12 Published online:2013-04-23

摘要:

为明确普通小麦中国春簇毛麦整臂互补易位系T1DS?1VLT1DL?1VS农艺特性和加工品质的效应, 20082010年在陕西杨凌连续2个生长季对这2个易位系和CS的主要农艺性状和加工品质性状进行了研究, 并采用SDS-PAGE法对簇毛麦高分子量麦谷蛋白亚基(HMW-GS)基因进行了进一步的染色体臂定位。结果表明, 2个易位系的抽穗期和成熟期相同, 但比CS1~2 d, T1DS?1VL其他农艺性状与中国春相似, T1DL?1VS的春季单株分蘖、千粒重和单株粒重显著高于中国春2个易位系的籽粒蛋白质含量与中国春无显著差异, T1DS?1VLZeleny沉淀值、面团形成时间、稳定时间和粉质仪质量指数显著降低;相反, T1DL?1VS的这些性状值显著提高。说明T1DS?1VL易位系对小麦的面团强度有显著的负向效应, T1DL?1VS易位系显著增强面筋强度。SDS-PAGE分析结果表明, 簇毛麦的1VS1VL上均含有簇毛麦的HMW-GS基因Glu-V1, 但该基因在T1DL?1VS中的表达可能强于在T1DS?1VL

关键词: 中国春, 簇毛麦, 易位系, 农艺性状, 加工品质

Abstract:

To understand the effect on agronomic traits and grain processing quality of Chinese Spring (CS)–Dasypyrum villosa compensating Robertsonian translocation lines T1DL?1VS and T1DS?1VL, we conducted a 2-year field trial at Yangling, Shaanxi Province from 2008 to 2011. Some agronomic traits and processing quality characteristics of T1DL?1VS and T1DS?1VL were compared with those of CS, and the high-molecular-weight glutein subunit (HMW-GS) genes of D. villosum were located on chromosome arms using SDS-PAGE method. The heading and maturity dates of both translocation lines were the same, but 1–2 d later than those of CS. Compared to CS, T1DS?1VL had similar other agronomic traits; however, T1DL?1VS showed higher values of spring tillers per plant, 1000-grain weight, and grain weight per plant. Although the seed protein contents of both translocation lines were not significantly different from those of CS, T1DS?1VL exhibited significantly lower Zeleny sedimentation value, developing time, stability, and quality index of farinograph than CS; the values of these quality parameters were significantly higher in T1DL?1VS than in CS. Therefore, T1DS?1VL has a significant negative effect on wheat dough strength, and T1DL?1VS significantly increase gluten strength. HMW-GS gene Glu-V1 of D. villosum was located on both of 1VS and 1VL, but the gene expression level might be higher in T1DL?1VS than in T1DS?1VL.

Key words: Chinese Spring, D. villosum, Translocation lines, Agronomic traits, Processing quality

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