作物学报 ›› 2020, Vol. 46 ›› Issue (4): 503-512.doi: 10.3724/SP.J.1006.2020.94082
HENG You-Qiang,YOU Xi-Long,WANG Yan()
摘要:
为明确一年生草本盐生植物费尔干猪毛菜(Salsola ferganica Drob.)病程相关蛋白基因SfPR1a (GenBank登录号为JQ670917)是否参与了植物对逆境胁迫的响应, 采用qRT-PCR检测了该基因在不同组织部位和脱落酸(ABA)、茉莉酸(JAs)、乙烯合成直接前体(ACC)等相关激素胁迫及NaCl处理下的表达规律, 同时对转基因烟草在盐、旱及丁香假单胞菌等胁迫下的抗性进行了鉴定。结果显示, SfPR1a基因在费尔干猪毛菜根中的表达量显著高于茎叶中, 且受到ABA、JAs、ACC、NaCl的积极诱导; 干旱胁迫下, 转基因烟草的丙二醛(MDA)含量显著低于野生型烟草, 显示出较强的抗旱表型; 盐胁迫下, 异源表达SfPR1a的转基因烟草幼苗生长显著优于野生型烟草; 丁香假单胞菌攻毒后的转基因烟草叶片呈现严重的坏死反应, 但植株的整体抗性表型显著优于野生型烟草; 亚细胞定位结果显示该蛋白定位于植物细胞质外体空间。以上结果表明, 费尔干猪毛菜病程相关蛋白SfPR1a基因参与了植物对非生物及生物胁迫的抗性。
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