甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

doi:10.3724/SP.J.1006.2020.94171

作物学报 ›› 2020, Vol. 46 ›› Issue (6): 844-857.doi: 10.3724/SP.J.1006.2020.94171

• 作物遗传育种·种质资源·分子遗传学 • 上一篇下一篇

甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

郑清雷¹,余陈静¹,姚坤存¹,黄宁¹,阙友雄^1,⁴,凌辉^2,^3,^*(),许莉萍^1,^4,^*()

¹福建农林大学 / 农业农村部福建甘蔗生物学与遗传育种重点实验室, 福建福州 350002
²福建农林大学生命科学学院, 福建福州 350002
³玉林师范学院农学院, 广西玉林 537000
⁴福建农林大学 / 教育部作物遗传育种与综合利用重点实验室, 福建福州 350002

收稿日期:2019-11-10 接受日期:2020-01-15 出版日期:2020-06-12 网络出版日期:2020-02-17
通讯作者: 凌辉,许莉萍
作者简介:E-mail: zhengqinglei1666@163.com.
基金资助:
国家自然科学基金项目(31801424);国家现代农业产业技术体系建设专项(CARS-17)

Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC

ZHENG Qing-Lei¹,YU Chen-Jing¹,YAO Kun-Cun¹,HUANG Ning¹,QUE You-Xiong^1,⁴,LING Hui^2,^3,^*(),XU Li-Ping^1,^4,^*()

¹Key Laboratory of Sugarcane Biology and Genetic Breeding (Fujian), Ministry of Agriculture and Rural Area / Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
²College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China
³College of Crop Science, Yulin Normal University, Yulin 537000, Guangxi, China
⁴Key Laboratory of Crop Genetics and Breeding and Comprehensive Utilization, Ministry of Education / Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian, China

Received:2019-11-10 Accepted:2020-01-15 Published:2020-06-12 Published online:2020-02-17
Contact: Hui LING,Li-Ping XU
Supported by:
National Natural Science Foundation of China(31801424);China Agriculture Research System(CARS-17)

摘要/Abstract

摘要：

细胞色素b₆f复合体还原型铁硫蛋白前体(Cytochrome b₆f complex Rieske Fe/S precursor protein, PetC)是由细胞核PetC基因编码的蛋白, 其成熟蛋白参与构成细胞色素b₆f复合体, 是电子传递的重要元件。基于前期构建的受高粱花叶病毒(Sorghum mosaic virus, SrMV)侵染的甘蔗转录组数据库, 从主栽甘蔗品种‘新台糖22号’叶片中成功克隆到该基因, 并命名为ScPetC (GenBank登录号为MH333037.1)。生物信息学分析发现, ScPetC基因cDNA全长824 bp, 包含了一个678 bp的开放阅读框, 编码226个氨基酸。ScPetC属于PRK13473超家族, 其C末端具有典型的Rieske保守结构域; 蛋白理论等电点为8.19, 属于稳定的、亲水性蛋白; 二级结构多为无规则卷曲, 三级结构预测其比其他植物PetC多出一段α螺旋结构。在本氏烟(Nicotiana benthamiana)叶片瞬时表达中, ScPetC定位于叶绿体、细胞质和细胞膜。尽管前人研究表明, ScPetC的表达量会受SrMV侵染的影响, 不同于半夏(Pinellia ternata) PetC与大豆花叶病毒(Soybean mosaic virus, SMV) P1蛋白之间的互作, ScPetC不能与SrMV-P1蛋白互作, 但能与甘蔗黄叶病毒(Sugarcane yellow leaf virus, SCYLV) P0蛋白互作。实时荧光定量PCR分析表明, ScPetC基因在甘蔗不同组织中均有表达, 但在叶片中的表达量最高。甘蔗受脱落酸胁迫3 h时, ScPetC表达量显著上调, 但是随着处理时间的延长, 表达受到抑制; 在茉莉酸甲酯、水杨酸、氯化铜、氯化镉和氯化钠胁迫下, ScPetC表达量均显著下调。本研究通过对ScPetC生物学功能、环境外源激素与重金属等胁迫下的表达模式及其与甘蔗病原病毒蛋白互作的初步探究, 增加了对甘蔗PetC的了解, 也为深入研究其在甘蔗受黄叶病毒侵染中的作用奠定基础。

关键词: 甘蔗, 细胞色素b₆f复合体还原型铁硫蛋白前体, 生物信息学, 亚细胞定位, 蛋白互作, 实时荧光定量PCR

Abstract:

Cytochrome b₆f complex Rieske Fe/S precursor protein (PetC) is encoded by the nuclear PetC gene, and its mature protein involved in the formation of the cytochrome b₆f complex, which is important for electron transfer. Based on our previous transcriptome data of sugarcane (Saccharum spp. hybrids) infected by Sorghum mosaic virus (SrMV), a cytochrome b₆f complex reduced iron-sulfur precursor protein gene was cloned from leaves of sugarcane superior elite cultivar ‘ROC22’, and named as ScPetC (GenBank accession number: MH333037.1). Bioinformatics analysis showed that the ScPetC gene was 824 bp in length, containing a 678 bp open reading frame (ORF), and encoding a peptide of 226 amino acids. ScPetC belongs to the PRK13473 superfamily and has a typical Rieske domain at its C-terminus of the amino acid chain. ScPetC is a stable and hydrophilic protein with pI 8.19. Most of the secondary structural elements in ScPetC were random coil. Compared to the PetC from the other plants, ScPetC contained one more fragment of helix in its third dimensional structure. The transient expression of YFP-fused protein in Nicotiana benthamiana leaves showed that ScPetC was located in the chloroplast, cytoplasm and cell membrane. Although the previous study indicated that the expression of ScPetC was affected by SrMV infection in sugarcane, different to the interaction between Pinellia ternata PetC and SMV-P1, without interaction between ScPetC and the SrMV-P1, it did interact with SCYLV-P0, i.e. the P0 protein of Sugarcane yellow leaf virus (SCYLV). Real-time quantitative PCR analysis showed that ScPetC gene was expressed constitutively in different tissues of sugarcane, and the highest level of its expression was found in leaves. When sugarcane plant was exposed to abscisic acid stress for 3 h, the expression of ScPetC was significantly up-regulated, but the expression level was then inhibited with longer treatment time. Under the stress of methyl jasmine, salicylic acid, copper chloride, cadmium chloride and sodium chloride, the expression of ScPetC was significantly down-regulated. The study on biological function, expression pattern and interaction of ScPetC with the proteins of sugarcane pathogenic virus will improve the understanding of the happening of yellow leaf symptom, which caused by SCYLV.

Key words: sugarcane, cytochrome b₆f complex Rieske Fe/S precursor protein, bioinformatics, subcellular location, protein interaction, real-time flourescent quantitative PCR

郑清雷,余陈静,姚坤存,黄宁,阙友雄,凌辉,许莉萍. 甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析[J]. 作物学报, 2020, 46(6): 844-857.

ZHENG Qing-Lei,YU Chen-Jing,YAO Kun-Cun,HUANG Ning,QUE You-Xiong,LING Hui,XU Li-Ping. Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC[J]. Acta Agronomica Sinica, 2020, 46(6): 844-857.

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://zwxb.chinacrops.org/CN/10.3724/SP.J.1006.2020.94171

https://zwxb.chinacrops.org/CN/Y2020/V46/I6/844

图/表 11

表1

表2

图1

表3

图2

图3

图4

图5

图6

图7

图8

参考文献 47

[1]	Yadav R L, Solomon S . Potential of developing sugarcane by product based industries in India. Sugar Tech, 2006,8:104-111.
[2]	何炎森, 李瑞美 . 甘蔗花叶病研究现状. 中国糖料, 2006, ( 1):47-49.
	He Y S, Li R M . Research status of Sugarcane mosaic virus disease in China. Sugar Crops China, 2006, ( 1):47-49 (in Chinese with English abstract).
[3]	iaz O D L C A, Hechavarra M D L L O, Izaguirre Y P, Navarro M D L A Z, Lopez J M M L, Lissbrant S, Arencibia A . Genotyping of Sugarcane yellow leaf virus in commercial cultivars and the Cuban germplasm collection. Sugar Tech, 2016,19:102-108.
[4]	王伯辉 . 我国甘蔗病害的发生现状与研究进展. 中国糖料, 2007, ( 3):261-265.
	Wang B H . The occurrence status and research progress of surgarcane disease in China. Sugar Crops China, 2007, ( 3):261-265 (in Chinese with English abstract).
[5]	Shukla D D, Frenkel J M, McKern M N, Ward C W, Jilka J, Tosic M, Ford R E, . Present status of the sugarcane mosaic subgroup of potyviruses. Arch Virol Suppl, 1992,5:363-373.
[6]	Elsayed A I, Komor E . Investigation of ORF0 as a sensitive alternative diagnostic segment to detect Sugarcane yellow leaf virus. J Gen Plant Pathol, 2012,78:207-216.
[7]	Mirkov T E, Ingelbrecht I, Castillon A, Hernandez J C . Identification of sugarcane proteins involved in posttranscriptional gene silencing. Proc Int Soc Sugarcane Technol, 2001,24:327-330.
[8]	Shan H Y, Pasin F, Valli A, Castillo C, Rajulu C, Carbonell A, Mateo S C, García J A, Rodamilans B . The Potyviridae P1a leader protease contributes to host range specificity. Virology, 2015,476:264-270.
[9]	Fabio P, Carmen S M, Antonio G J . The hypervariable amino terminus of P1 protease modulates potyviral replication and host defense responses. PLoS Pathog, 2014,10:e1003985. doi 10.1371/journal.ppat.1003985. doi: 10.1371/journal.ppat.1003985
[10]	Mangwende T, Wang M L, Borth W, Hu J, Moore P H, Mirkov T E, Albert H H . The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities. Virology, 2009,384:38-50. doi: 10.1016/j.virol.2008.10.034
[11]	Veidt I, Bouzoubaa S E, Leiser R M, Zieglergraff V A, Guilley H, Richards K, Jonard G . Synthesis of full-length transcripts of Beet western yellows virus RNA messenger properties and biological activity in protoplasts. Virology, 1992,186:192-200. doi: 10.1016/0042-6822(92)90073-X
[12]	燕照玲, 段俊枝, 冯丽丽, 陈海燕, 齐红志, 杨翠苹, 施艳, 张会芳 . 马铃薯Y病毒属病毒编码蛋白与寄主植物叶绿体蛋白互作研究进展. 河南农业科学, 2017,46(11):1-5.
	Yan Z L, Duan J Z, Feng L L, Chen H Y, Qi Z H, Yang C P, Shi Y, Zhang H F . Progress on interaction between potyviruses coded proteins and chloroplast proteins of host plants. J Henan Agric Sci, 2017,46(11):1-5 (in Chinese with English abstract).
[13]	Zhao J P, Zhang X, Hong Y G, Liu Y L . Chloroplast in plant virus interaction. Front Microbiol, 2016,7:1565. doi: 10.3389/fmicb.2016.01565.
[14]	Lin L, Luo Z P, Yan F, Lu Y W . Interaction between potyvirus P3 and ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of host plants. Virus Genes, 2011,43:90-92. doi: 10.1007/s11262-011-0596-6
[15]	翟玉山, 赵贺, 张海, 邓宇晴, 程光远, 杨宗桃, 王彤, 彭磊, 徐倩, 董萌, 徐景升 . 甘蔗NAD(P)H脱氢酶复合体O亚基基因克隆及其与甘蔗花叶病毒VPg互作研究, 作物学报, 2019,45:1478-1487. doi: 10.3724/SP.J.1006.2019.94002
	Zhai Y S, Zhao H, Zhang H, Deng Y Q, Cheng G Y, Yang Z T, Wang T, Peng L, Xu Q, Dong M, Xu J S . Cloning of NAD(P)H complex O subunit gene and its interaction with VPg of Sugarcane mosaic virus. Acta Agron Sin, 2019,45:1478-1487 (in Chinese with English abstract). doi: 10.3724/SP.J.1006.2019.94002
[16]	Shi Y, Chen J, Hong X, Chen J, Adams M J . A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host. Mol Plant Pathol, 2010,8:785-790. doi: 10.1111/mpp.2007.8.issue-6
[17]	Pazhouhandeh M, Dieterle M, Marrocco K, Lechner E, Berry B, Brault V, Hemmer O, Kretsch T, Richards K E, Genschik P, Graff V Z . F-box-like domain in the polerovirus protein P0 is required for silencing suppressor function. Proc Natl Acad Sci USA, 2006,103:1994-1999. doi: 10.1073/pnas.0510784103
[18]	Sun Q Q, Li Y Y, Wang Y, Zhao H H, Zhao T Y, Zhang Z Y, Li D W, Yu J L, Wang X B, Zhang Y L, Han C G . BrYV P0 protein impairs the antiviral activity of NbRAF2 in Nicotiana Benthamiana. J Exp Bot, 2018,69:3127-3139. doi: 10.1093/jxb/ery131
[19]	李合生 . 现代植物生理学(第3版). 北京: 高等教育出版社, 2012. pp 114-118.
	Li H S . Modern Plant Physiology, 3rd edn. Beijing: Higher Education Press, 2012. pp 114-118(in Chinese).
[20]	Schottler M A, Tóth S Z, Boulouis A . Photosynthetic complex stoichiometry dynamics in higher plants: biogenesis, function, and turnover of ATP synthase and the cytochrome b₆f complex. J Exp Bot, 2015,66:2373-2400. doi: 10.1093/jxb/eru495
[21]	Rieske J S, Zaugg W S, Hansen R E . Studies on the electron transfer system. J Biol Chem, 1964,242:2051-2061.
[22]	Rieske J S . Composition, structure, and function of complex III of the respiratory chain. Biochim Biophys Acta, 1976,456:195-247.
[23]	Steppuhn J, Rother C, Hermans J, Jansen T . The complete amino acid sequence of the Rieske Fe/S precursor protein from spinach chloroplasts deduced from cDNA analysis. Mol Gen Genet, 1987,210:171-177. doi: 10.1007/BF00337775
[24]	Salter A H, Newman B J, Napier J, Gray J C . Import of the precursor of the chloroplast Rieske iron-sulfur protein by pea chloroplasts. Plant Mol Biol, 1992,20:569-574. doi: 10.1007/BF00040617
[25]	Knight J S, Duckett C M, Sullivan J A, Walker A R, Gray J C . Tissue-specific, light-regulated and plastid-regulated expression of the single-copy nuclear gene encoding the chloroplast Rieske Fe/S protein of Arabidopsis thaliana. Plant Cell Physiol, 2002,43:522-531. doi: 10.1093/pcp/pcf062
[26]	Madueno F, Bradshaw S A, Gray J C . The thylakoid-targeting domain of the chloroplast Rieske iron-sulfur protein is located in the N-terminal hydrophobic region of the mature protein. J Biol Chem, 1994,26:17458-17463.
[27]	Vitry C D . Characterization of the gene of the chloroplast Rieske iron-sulfur protein in Chlamydomonas reinhardtii indications for an uncleaved lumen targeting sequence. J Biol Chem, 1994,269:7603-7609.
[28]	Carrell C J, Zhang H, Cramer W A, Smith J L . Biological identity and diversity in photosynthesis and respiration: structure of the lumen side domain of the chloroplast Rieske protein. Structure, 1997,5:1613-1625.
[29]	Hasan S S, Yamashita E, Cramer W A . Transmembrane signaling and assembly of the cytochrome b₆f-lipidic charge transfer complex. Biochim Biophys Acta, 2013,1827:1295-1308.
[30]	Cramer W A, Hasan S S, Yamashita E, Kahlau S . The Q cycle of cytochrome bc₁ complexes: a structure perspective. Biochim Biophys Acta, 2011,1807:788-802.
[31]	Yamori W, Kondo E, Sugiura D, Terashima I, Suzuki Y, Makino A . Enhanced leaf photosynthesis as a target to increase grain yield: insights from transgenic rice lines with variable Rieske Fe/S protein content in the cytochrome b₆f complex. Plant Cell Environ, 2016,39:80-87.
[32]	Simkin A J, Lorna M, Lawson T, Raines C A . Over-expression of the Rieske Fe/S protein increases electron transport rates and biomass yield. Plant Physiol, 2017,175:134-145.
[33]	Sanda S, Munekage Y N, Yoshida K, Akashi K, Yokota A . Excess light-induced molecular responses of chloroplast Rieske Fe/S protein. Photosynth Energy Sun, 2008,14:941-944.
[34]	梁潘霞, 李杨瑞 . 甘蔗细胞色素b₆f复合体铁硫亚基(SoCYT)基因的克隆和表达分析. 西南农业学报, 2016,29:1032-1037.
	Liang P X, Li L R . Cloning of cytochrome b6/f complex iron-sulfur subunit( SoCYT) from sugarcane and analysis of its expression under PEG stress. Acta Agric Boreali-occident Sin, 2016,29:1032-1037 (in Chinese with English abstract).
[35]	Luo M, Kong X Y, Jiang T, Jia C, Zhou R H, Jia J Z . Analysis of resistance to powdery mildew in wheat based on Expressed Sequence Tags (EST) technique. Acta Bot Sin, 2002,44:567-572.
[36]	阙友雄, 林剑伟, 徐景升, 阮秒鸿, 许莉萍, 张木清, 陈由强, 陈如凯 . 甘蔗与黑穗病菌互作的叶片差异蛋白分析. 热带作物学报, 2008,29:136-140.
	Que Y X, Lin W J, Xu J S, Ruan M H, Xu L P, Zhang M Q, Chen Y Q, Chen R K . Analysis of protein expression patterns of leaves from interaction between sugarcane and Ustilago scitaminea. Chin J Trop Crops, 2008,29:136-140 (in Chinese with English abstract).
[37]	Ling H, Huang N, Wu Q B, Su Y C, Peng Q, Ahmed W, Gao S W, Su W H, Que Y X, Xu L P . Transcriptional insights into the sugarcane- Sorghum mosaic virus interaction. Trop Plant Biol, 2018,11:163-176.
[38]	黄宁, 张玉叶, 凌辉, 罗俊, 吴期滨, 阙友雄 . 甘蔗二氨基庚二酸异构酶基因的克隆与表达分析. 热带作物学报, 2013,34:2200-2208.
	Huang N, Zhang Y Y, Ling H, Luo J, Wu Q B, Que Y X . Cloning and expression analysis of a diaminopimelate epimerase gene in sugarcane. Chin J Trop Crops, 2013,34:2200-2208 (in Chinese with English abstract).
[39]	苏亚春, 黄珑, 凌辉, 王竹青, 刘峰, 苏炜华, 黄宁, 吴期滨, 高世武, 阙友雄 . 甘蔗CDK基因的cDNA全长克隆与表达分析. 作物学报, 2017,43:42-50.
	Su Y C, Huang L, Ling H, Wang Z Q, Liu F, Su W H, Huang N, Wu Q B, Gao S W, Que Y X . Cloning and expression analysis of CDK Gene in sugarcane. Acta Agron Sin, 2017, 43:42-50 (in Chinese with English abstract).
[40]	Liu F, Huang N, Wang L, Ling H, Sun T T, Ahmad W, Muhammad K, Guo J X, Xu L P, Gao S W, Que Y X, Su Y C . A novel L-ascorbate peroxidase 6 gene, ScAPX6, plays an important role in the regulation of response to biotic and abiotic stresses in sugarcane. Front Plant Sci, 2017,8:2262. doi: 10.3389/fpls.2017.02262.
[41]	Schmidt C L, Shaw L . A comprehensive phylogenetic analysis of Rieske and Rieske-type iron-sulfur proteins. J Bioenerg Biomembr, 2001,33:9-26.
[42]	Schütze K, Harter K, Chaban C . Bimolecular fluorescence complementation (BiFC) to study protein-protein interactions in living plant cells. Metho Mol Biol, 2009,479:189-202.
[43]	Guo J L, Ling H, Wu Q B, Xu L P, Que Y X . The choice of reference genes for assessing gene expression in sugarcane under salinity and drought stresses. Sci Rep, 2014,4:7042. doi: 10.1038/srep07042.
[44]	郑红英 . TuMV HC-Pro蛋白自身互作及其与拟南芥编码Rieske Fe/S蛋白的互作研究. 华中农业大学博士学位论文, 湖北武汉, 2011.
	Zheng H Y . The Self Interaction of TuMV HC-Pro and Its Interaction with Rieske Fe/S Protein Encoded by Arabidopsis thaliana. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2011 (in Chinese with English abstract).
[45]	Dashevskaya S, Horn R, Chudobova I, Schillberg S, Velez S M R, Capell T, . Abscisic acid and the herbicide safener cyprosulfamide cooperatively enhance abiotic stress tolerance in rice. Mol Breed, 2013,32:463-484.
[46]	Liu B, Xue X D, Cui S P, Zhang X Y, Han Q M, Zhu L, Liang X F, Wang X J, Huang L L, Chen X M, Kang Z S . Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici. Mol Biol Rep, 2010,37:1045-1052.
[47]	严蓓 . 钙缓解黄瓜幼苗NaCl胁迫伤害的蛋白质组学及光合碳代谢研究. 南京农业大学硕士学位论文,江苏南京, 2013.
	Yan P . Proteomics and Photosynthetic Carbon Metabolism Research on Calcium Alleviating Salt Stress of Cucumber Seedlings. MS Thesis of Nanjing Agricultural University, Nanjing, Jiangsu, China, 2013 (in Chinese with English abstract).

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

引物名称 Primer name	引物序列 Primer sequence (5°-3°)	用途 Usage
ScPetC-F	TGGACGCCAGGGAGTGAAC	基因克隆 Gene cloning
ScPetC-R	TGTAGCAACAACGCCTTCGG	基因克隆 Gene cloning
ScPetC-QF	GTACAACAACCAGGGCAAGG	实时荧光定量PCR qRT-PCR
ScPetC-QR	CAGGGACAGGAGGCATACAT	实时荧光定量PCR qRT-PCR
CAC-F	ACAACGTCAGGCAAAGCAAA	内参基因 Reference gene
CAC-R	AGATCAACTCCACCTCTGCG	内参基因 Reference gene
CUL-F	TGCTGAATGTGTTGAGCAGC	内参基因 Reference gene
CUL-R	TTGTCGCGCTCCAAGTAGTC	内参基因 Reference gene
ScPetC-Subloc-F	CGAACGATACTCGAGGTCGACATGGCGACCTCCGCG	亚细胞定位载体 Vector for subcellular localization
ScPetC-Subloc-R	GCTCACCATACTAGTGGATCCTGCCTTCCACCACGG	亚细胞定位载体 Vector for subcellular localization
ScPetC-AD-F	CCATGGAGGCCAGTGAATTCATGGCGACCTCCGCGGCGCT	酵母双杂交载体 Vector for Y2H
ScPetC-AD-R	AGCTCGAGCTCGATGGATCCTGCCTTCCACCACGGGTCCT	酵母双杂交载体 Vector for Y2H
SrMV-P1-BK-F	TGGCCATGGAGGCCGAATTCATGGCAGGAGCATGGAGCAC	酵母双杂交载体 Vector for Y2H
SrMV-P1-BK-R	CGCTGCAGGTCGACGGATCC AAAATAAGTAATTTCATTGA	酵母双杂交载体 Vector for Y2H
SCYLV-P0-BK-F	TGGCCATGGAGGCCGAATTCATGCTTTTCAACGAATTCTC	酵母双杂交载体 Vector for Y2H
SCYLV-P0-BK-R	CGCTGCAGGTCGACGGATCCTATATCATGAGAATAGGTGC	酵母双杂交载体 Vector for Y2H
ScPetC-YN-F	TCTGAGGAGGATCTTCCCGGGATGGCGACCTCCGCGGCGCT	双分子荧光互补实验载体 Vector for BiFC
ScPetC-YN-R	AGGGCATGCCTGCAGGTCGACGTGCCTTCCACCACGGGTCCT	双分子荧光互补实验载体 Vector for BiFC
SrMV-P1-YC-F	TCTAGGAGCTCGGTACCCGGGATGGCAGGAGCATGGAGCAC	双分子荧光互补试验载体 Vector for BiFC
SrMV-P1-YC-R	ATCGTATGGGTACATACTAGTAAAATAAGTAATTTCATTGA	双分子荧光互补试验载体 Vector for BiFC
SCYLV-P0-YC-F	CTAGGAGCTCGGTACCCGGGATGCTTTTCAACGAATTCTC	双分子荧光互补实验载体 Vector for BiFC
SCYLV-P0-YC-R	TCGTATGGGTACATACTAGTTATATCATGAGAATAGGTGC	双分子荧光互补实验载体 Vector for BiFC

工具Tool	网址Website
ORF Finder	https://www.ncbi.nlm.nih.gov/orffinder/
ExPaSy	http://web.expasy.org/protparam/
Prabi	https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_hnn.html
SWISSMODEL	http://swissmodel.expasy.org/interactive
SignalP 4.1 Server	http://www.cbs.dtu.dk/servers/SignalP/
NCBI conserved domain	http: //blast.ncbi.nlm.nih.gov/
WoLFPSORT	https://wolfpsort.hgc.jp/
TMHMM 2.0 Server	http://www.cbs.dtu.dk/servers/TMHMM/
ProtScale	https://web.expasy.org/protscale/
MEME	http://meme-suite.org/

一级结构特性Primary structure characteristic	预测结果Predicted result
编码的氨基酸个数Number of coding amino acids	225
等电点 Theoretical pI	8.19
分子量 Molecular weight (×10³)	23.85
分子式 Formula	C₁₀₅₄H₁₆₆₈N₂₉₄O₃₁₅S₁₁
不稳定系数 Instability index (II)	21.61
平均疏水性Grand average of hydropathicity (GRAVY)	-0.146

甘蔗Rieske Fe/S蛋白前体基因ScPetC的克隆及表达分析

Cloning and expression analysis of sugarcane Fe/S precursor protein gene ScPetC

RichHTML

PDF

摘要/Abstract

引用本文

使用本文

图/表 11

参考文献 47

相关文章 15

Metrics

本文评价

推荐阅读 0

关于本刊

在线期刊

作者中心

相关单位

联系我们

[1]	崔致远, 秦晨展, 刘星雨, 张海, 曾康, 黄国强, 徐景升. 甘蔗类四跨膜蛋白ScTSPAN18与6K2互作应答SCMV侵染研究[J]. 作物学报, 2026, 52(6): 1618-1630.
[2]	胡赵, 钱润, 谢丰璞, 应素平. 水稻SPX基因家族鉴定及响应磷处理的表达分析[J]. 作物学报, 2026, 52(6): 1902-1912.
[3]	田春艳, 陆鑫, 吴才文, 徐超华, 刘家勇, 边芯, 桃联安. 基于荧光SSR的甘蔗创新种质遗传多样性分析及育种潜力评估[J]. 作物学报, 2026, 52(4): 1057-1072.
[4]	牛丽, 王勇胜, 王长杰, 张宏, 孟亚雄, 李葆春, 杨轲, 马小乐, 姚立蓉, 司二静, 王化俊, 汪军成. 大麦NAC基因家族鉴定分析及HvNAC38的耐盐功能验证[J]. 作物学报, 2026, 52(3): 688-707.
[5]	杨宗桃, 杨婷, 王禹童, 艾静, 李燕烨, 刘家勇, 邓军, 赵勇, 张跃彬. 甘蔗CLC基因家族鉴定与表达分析[J]. 作物学报, 2026, 52(3): 722-734.
[6]	于永超, 刘明, 靳容, 赵鹏, 张强强, 王静, 朱晓亚, 唐忠厚. 甘薯高氮徒长的生理机制和转录组分析研究[J]. 作物学报, 2026, 52(3): 813-824.
[7]	覃奕琰, 付瑶, 苏畅, 李娜, 徐静茹, 程笑然, 张琪, 赵明辉. OsST41调控水稻苗期耐盐性的功能分析[J]. 作物学报, 2026, 52(3): 802-812.
[8]	张晴, 杨昱, 郭茜, 岳霈尧, 殷丛丛, 牛景萍, 赵晋忠, 杜维俊, 岳爱琴. 大豆GmARA6a的克隆及响应盐胁迫的功能分析[J]. 作物学报, 2026, 52(2): 480-493.
[9]	余开航, 周洪斌, 罗亮扎, 王玫郦, 姜瑞梅, 董陈文华, 李仕金, 毛孝强, 陈升位. 大麦亮氨酸富集重复型类受体激酶基因HvLRR-RLK-510的克隆和表达分析[J]. 作物学报, 2026, 52(2): 421-432.
[10]	万慧兰, 吴华英, 曾丹, 钱禛锋, 赵昌祖, 廖然超, 何丽莲, 李富生. 蔗茅耐寒相关基因EfWRKY51克隆分析及功能验证[J]. 作物学报, 2025, 51(8): 2048-2059.
[11]	刘晓宁, 张颖, 蔡曼蕾, 马昊, 苗智博, 曹宁, 连荣芳, 徐全乐. LsSAT2与LsAAE3互作调控山黧豆ODAP水平[J]. 作物学报, 2025, 51(8): 2220-2227.
[12]	凤舞剑, 冼晓青, 张新钵, 曹丹, 强承魁. 基于转录组和AlphaFold对稻瘟菌经典效应蛋白和水稻受体的快速鉴定[J]. 作物学报, 2025, 51(6): 1480-1488.
[13]	周恩强, 缪亚梅, 周瑶, 姚梦楠, 赵娜, 王永强, 朱宇翔, 薛冬, 李宗迪, 石宇欣, 李波, 汪凯华, 顾春燕, 王学军, 魏利斌. 基于种子发育转录组的豌豆bZIP基因家族分析及种子发育候选基因的鉴定[J]. 作物学报, 2025, 51(4): 914-931.
[14]	潘炬忠, 韦萍, 朱德平, 邵胜雪, 陈珊珊, 韦雅倩, 高维维. 水稻转录因子OsERF104的克隆和功能研究[J]. 作物学报, 2025, 51(4): 900-913.
[15]	张恒, 冯雅岚, 田文仲, 郭彬彬, 张均, 马超. 小麦TaSnRK基因家族鉴定及在局部根区干旱下的表达分析[J]. 作物学报, 2025, 51(3): 632-649.