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作物学报 ›› 2015, Vol. 41 ›› Issue (09): 1454-1461.doi: 10.3724/SP.J.1006.2015.01454

• 研究简报 • 上一篇    

玉米茉莉酸甲酯不敏感突变体的筛选

查象敏1, 汪海1, 路小铎2, 张春义1, 黄大昉1, *, 郎志宏1, *   

  1. 1中国农业科学院生物技术研究所, 北京 100081; 2齐鲁师范学院生命科学学院, 山东章丘 250200
  • 收稿日期:2015-02-09 出版日期:2015-09-12 网络出版日期:2015-09-12
  • 通讯作者: 黄大昉, E-mail:huangdafang@caas.cn; 郎志宏, E-mail:langzhihong@caas.cn
  • 基金资助:
    本研究由国家自然科学基金项目(31270317)资助

Screening of Methyl Jasmonate-Insensitive Mutants in Maize

ZHA Xiang-Min1, WANG Hai1, LU Xiao-Duo2, ZHANG Chun-Yi1, HUANG Da-Fang1, *, LANG Zhi-Hong1, *   

  1. 1 Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 School of Life Sciences, Qilu Normal University, Zhangqiu 250200, China
  • Received:2015-02-09 Published:2015-09-12 Published online:2015-09-12

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摘要： 植物激素茉莉酸(jasmonic acid, JA)作为一种可以长距离运动的信号分子在植物对昆虫侵害的系统抗性中起核心调控作用。为了解析玉米JA信号途径及抗虫机制, 本研究利用改进的玉米突变体的筛选方法, 筛选与JA信号途径相关的突变体用于下一步的研究。将甲基磺酸乙酯(ethyl methane sulfonate, EMS)诱变的玉米突变体库6118份种子分别置含有外源茉莉酸甲酯(methyl jasmonate, MeJA)的培养基中培养, 通过观察玉米根系的生长情况, 确定对MeJA的不同耐受性。当MeJA终浓度为50 µmol L-1时, 初步筛选到对MeJA不敏感的I级耐受性突变体61株; 当MeJA终浓度分别为100 µmol L-1和200 µmol L-1时进行验证, 得到的耐受性事件分别为37株和10株。对自交收获的子一代遗传性分析表明, 这些突变体对MeJA表现出的耐受性具有可遗传性。筛选获得的对MeJA不敏感突变体可能与JA调控有关, 是研究玉米JA信号途径的很好材料。

关键词: MeJA/JA, 玉米突变体库筛选, MeJA不敏感, 玉米虫害应答

Abstract: The phytohormone jasmonate plays a pivotal role in plant systematic resistance to herbivores attack as a long-distance mobile signal. To demonstrate the Jasmonic acid (JA) signal pathway and the insect-resistant mechanism in maize, we utilized an improved approach to screen the maize mutants associated with JA signal pathway, which will be available for further research. The 6118 lines’ seeds from EMS-mutagenic maize mutants library were cultivated in the medium containing the exogenous MeJA. The different tolerances of the mutants to MeJA were identified by observing the growth of maize roots. When the final concentration of MeJA was 50 µmol L-1, 61 MeJA-insensitive mutants with tolerance Grade I were obtained in the primary screening experiment. When the final concentration of MeJA was 100 µmol L-1 or 200 µmol L-1, 37 and 10 tolerant events were gained in the confirmation experiments, respectively. Then, we analyzed the heredity of M1 seeds tolerant to MeJA. The results showed that the tolerance of the mutants to MeJA was hereditable. The MeJA-insensitive mutants associated with JA regulation pathway are the potential materials for the further studies of JA signal pathway and insect resistant mechanism in maize.

Key words: MeJA/JA, Screening maize mutants library, MeJA-insensitive, Insect attack response

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