作物学报 ›› 2015, Vol. 41 ›› Issue (09): 1454-1461.doi: 10.3724/SP.J.1006.2015.01454
• 研究简报 • 上一篇
查象敏1, 汪海1, 路小铎2, 张春义1, 黄大昉1, *, 郎志宏1, *
ZHA Xiang-Min1, WANG Hai1, LU Xiao-Duo2, ZHANG Chun-Yi1, HUANG Da-Fang1, *, LANG Zhi-Hong1, *
摘要: 植物激素茉莉酸(jasmonic acid, JA)作为一种可以长距离运动的信号分子在植物对昆虫侵害的系统抗性中起核心调控作用。为了解析玉米JA信号途径及抗虫机制, 本研究利用改进的玉米突变体的筛选方法, 筛选与JA信号途径相关的突变体用于下一步的研究。将甲基磺酸乙酯(ethyl methane sulfonate, EMS)诱变的玉米突变体库6118份种子分别置含有外源茉莉酸甲酯(methyl jasmonate, MeJA)的培养基中培养, 通过观察玉米根系的生长情况, 确定对MeJA的不同耐受性。当MeJA终浓度为50 µmol L-1时, 初步筛选到对MeJA不敏感的I级耐受性突变体61株; 当MeJA终浓度分别为100 µmol L-1和200 µmol L-1时进行验证, 得到的耐受性事件分别为37株和10株。对自交收获的子一代遗传性分析表明, 这些突变体对MeJA表现出的耐受性具有可遗传性。筛选获得的对MeJA不敏感突变体可能与JA调控有关, 是研究玉米JA信号途径的很好材料。
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