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作物学报 ›› 2008, Vol. 34 ›› Issue (10): 1679-1687.doi: 10.3724/SP.J.1006.2008.01679

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

转Xa23基因水稻的白叶枯病抗性及其遗传分析

张小红1,2,**;王春连2,3,**;李桂芬2;张晓科1;梁云涛2;孙亮庆2;赵开军2,3,*   

  1. 1 西北农林科技大学, 陕西杨凌712100; 2 中国农业科学院作物科学研究所 / 农业部作物遗传育种重点实验室, 北京100081; 3 国家农作物基因资源与基因改良重大科学工程, 北京100081
  • 收稿日期:2008-02-26 修回日期:1900-01-01 出版日期:2008-10-12 网络出版日期:2008-10-12
  • 通讯作者: 赵开军

Genetic Analysis on Bacterial Blight Resistance of Xa23-Transgenic Rice

ZHANG Xiao-Hong12**,WANG Chun-Lian23**,LI Gui-Fen2,ZHANG Xiao-Ke1,LIANG Yun-Tao2,SUN Liang-Qing2,ZHAO Kai-Jun23*   

  1. 1 Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi; 2 Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081; 3 The National Key Facility for Crop Gene Resources and Genetic Improvement, Beijing 100081, China
  • Received:2008-02-26 Revised:1900-01-01 Published:2008-10-12 Published online:2008-10-12
  • Contact: ZHAO Kai-Jun

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摘要： 在分离克隆抗白叶枯病基因Xa23研究中获得大量转基因水稻材料。为了系统研究转Xa23基因水稻的抗病稳定性和遗传模式, 本文通过逐株进行抗白叶枯病接种鉴定、PCR和Southern blot分子检测, 对一批转Xa23基因水稻植株进行了T0代到T2代的跟踪分析。结果表明, Xa23基因的整合和表达, 使感病受体品种牡丹江8号获得抗病性。由于Xa23基因插入受体基因组的位点不同, 同是单拷贝插入的转基因T0代抗病植株, 其抗病程度有明显差异。T0代植株的抗病程度, 可以准确、稳定地遗传到T1代和T2代。单拷贝转基因植株分离群体的抗感植株分离比接近3∶1, 表明转Xa23基因遵循孟德尔单基因遗传模式。已获得2个纯合的单拷贝转基因抗病株系, 它们的抗病程度稍有差别, 将用于外源基因插入位置效应分析和杂交稻抗病育种。

关键词: 白叶枯病, Xa23基因, 转基因水稻

Abstract: Transgenic rice plants were previously generated by Agrobacterium-mediated transformation during our research on molecular cloning of Xa23 gene, a bacterial blight (BB) resistance gene from the wild rice species Oryza rufipogon. To investigate the stability and inheritance of the transgene and its corresponding BB-resistance phenotype, more than 350 Xa23-trangenic rice plants from T0, T1, and T2 generations were subjected to BB-pathogen inoculation, PCR and Southern blot analysis for the trans-gene. Results showed that, Xa23 gene was incorporated and expressed in the genome of the susceptible receptor rice variety Mu-danjiang 8 that acquired BB-resistance. Due to the difference of the transgene insertion sites, T0 plants with single-copy insertion performed quite different degrees of resistance and their resistance was accurately and stably inherited to T1 and T2 generations. We also observed that the transgene in the single-copy insertion transgenic lines inherited following the Mendelian rules. Two homozygous, single-copy insertion transgenic lines with different resistance degrees have been obtained.

Key words: Bacterial blight, Xa23, Transgenic rice

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