作物学报 ›› 2015, Vol. 41 ›› Issue (03): 386-393.doi: 10.3724/SP.J.1006.2015.00386
李定琴,陈玲,李维蛟,柯学,余腾琼,李娥贤,黄兴奇,程在全*
LI Ding-Qin,CHEN Ling,LI Wei-Jiao,KE Xue,YU Teng-Qiong,LI E-Xian,HUANG Xing-Qi,CHENG Zai-Quan*
摘要:
白叶枯病是水稻生产上最重要的细菌性病害之一,培育抗病新品种是防治白叶枯病最经济有效的途径。然而,栽培稻来源的抗病基因数量有限,并且部分抗病基因的抗病谱窄。因此,从野生稻中发掘抗病基因,将有利于培育抗病谱广且抗病能力强的水稻新品种。云南野生稻资源丰富,但其中是否含有已报道克隆的抗白叶枯病基因,目前还未见相关综合研究报道。本研究通过抗性鉴定和PCR分析,检测云南野生稻中的抗白叶枯病基因。结果表明,云南野生稻对两个代表性白叶枯病菌Y8和PXO99具有不同程度的抗性,疣粒野生稻甚至达到免疫的程度。3种野生稻中均不含xa5、xa13和Xa21抗病基因,元江普通野生稻含Xa23和Xa3/Xa26基因或其同源基因,景洪普通野生稻中含Xa1、Xa3/Xa26和Xa27基因或同源基因,药用野生稻含Xa3/Xa26基因或同源基因,而疣粒野生稻含有Xa27抗性基因。本研究结果为进一步发掘和克隆云南野生稻中的抗白叶枯病新基因提供了理论参考。
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