作物学报 ›› 2015, Vol. 41 ›› Issue (03): 378-385.doi: 10.3724/SP.J.1006.2015.00378
丁检,吴双,蔡彩平,郭旺珍*
DINGJian,WUShuang,CAICai-Ping,GUO Wang-Zhen*
摘要:
棉花油脂合成相关代谢在控制油分形成、纤维发育等进程中起着重要作用。溶血磷脂酰基转移酶(LPAT)是植物油脂代谢过程中的一个关键酶。本研究利用雷蒙德氏棉全基因组数据库,通过生物信息学技术,鉴定并获得8个棉花LPAT家族基因的全序列和染色体定位等信息。通过序列比对进行系统进化和分类分析,明确这8个家族基因分布在6条染色体上,分为4亚类,其中第I类和第III类中各含2个基因、第II类1个、第IV类3个。组织表达分析表明这8个基因在棉花营养和生殖阶段均表现表达多样性。LPAT6和LPAT8在17d胚珠中表达水平很高,推测在油脂合成代谢调控中起重要作用。8个LPAT基因均在纤维中优势表达,其中LPAT2、LPAT3、和LPAT4表达水平更高,推测LPAT家族基因的表达参与棉纤维发育进程。
[1]Chapman K D, Ohlrogge J B. Compartmentation of triacylglycerol accumulation in plants. J Biol Chem, 2012,287: 2288–2294[2]Weselake R J, Taylor D C, Rahman M H, Shah S, Laroche A, McVetty P B, Harwood J L. Increasing the flow of carbon into seed oil. Biotechnol Adv, 2009, 27: 866–878[3]Kim H U, Li Y, Huang A H. Ubiquitous and endoplasmic reticulum-located lysophosphatidyl acyltransferase, LPAT2, is essential for female but not male gametophyte development in Arabidopsis. Plant Cell, 2005, 17: 1073–1089[4]Arroyo-Caro J M, Chileh T, Kazachkov M, Zou J, Alonso D L, Garcia-Maroto F. The multigene family of lysophosphatidate acyltransferase (LPAT)-related enzymes in Ricinus communis: cloning and molecular characterization of two LPAT genes that are expressed in castor seeds. Plant Sci, 2013, 199-200: 29–40[5]陈四龙, 黄家权, 雷永, 任小平, 文奇根, 陈玉宁, 姜慧芳, 晏立英, 廖伯寿. 花生溶血磷脂酸酰基转移酶基因的克隆与表达分析. 作物学报, 2012, 38: 245–255Chen S L, Huang J Q, Lei Y, Ren X P, Wen Q G, Chen Y N, Jiang H F, Yan L Y, Liao B S. Cloning and expression analysis of lysophosphatidic acid acyltransferase (LPAT) encoding gene in peanut. Acta Agron Sin, 2012, 38: 245–255 (in Chinese with English abstract)[6]Roscoe TJ. Identification of acyltransferases controlling triacylglycerol biosynthesis in oilseeds using a genomics-based approach. Eur J Lipid Sci Tech, 2005, 107: 256–262[7]Kim H U, Huang A H. Plastid lysophosphatidyl acyltransferase is essential for embryo development in Arabidopsis. Plant Physiol, 2004, 134: 1206–1216[8]Ohlrogge J, Browse J. Lipid biosynthesis. Plant Cell, 1995, 7: 957–970[9]Zou J, Katavic V, Giblin E M, Barton D L, MacKenzie S L, Keller W A, Hu X, Taylor D C. Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell, 1997, 9: 909–923[10]Maisonneuve S, Bessoule J J, Lessire R, Delseny M, Roscoe T J. Expression of rapeseed microsomal lysophosphatidic acid acyltransferase isozymes enhances seed oil content in Arabidopsis. Plant Physiol, 2010, 152: 670–684[11]冷雪, 贾银华, 杜雄明. 棉纤维伸长阶段上、下调基因及相关通路的分析.作物学报, 2010,36: 1891–1901Leng X, Jia Y H, Du X M. Up- and down-regulated genes during cotton fiber elongation and relative pathway. Acta Agron Sin, 2010, 36: 1891–1901 (in Chinese with English abstract)[12]Qin Y M, Hu C Y, Pang Y, Kastaniotis A J, Hiltunen J K, Zhu Y X. Saturated very-long-chain fatty acids promote cotton fiber and Arabidopsis cell elongation by activating ethylene biosynthesis. Plant Cell, 2007, 19: 3692–3704[13]赵永国, 郭瑞星. 棉子含油量研究进展与高油棉花育种可行性分析. 棉花学报, 2011,23: 184–188Zhao Y G, Guo R X. Research progress on oil content of cottonseed and feasibility of high oil content breeding in Upland Cotton. Cotton Sci, 2011, 23: 184–188 (in Chinese with English abstract)[14]张欢, 孟永彪. 用棉籽油制备生物柴油. 化工进展, 2007, (1): 86–89Zhang H, Meng Y B. Mass production of bio-diesel from cottonseed oil via transesterification. Chem Ind Eng Prog, 2007, 1: 86–89 (in Chinese with English abstract)[15]Paterson A H, Wendel J F, Gundlach H, Guo H, Jenkins J, Jin D, Llewellyn D, Showmaker KC, Shu S, Udall J, Yoo MJ, Byers R, Chen W, Doron-Faigenboim A, Duke MV, Gong L, Grimwood J, Grover C, Grupp K, Hu G, Lee TH, Li J, Lin L, Liu T, Marler BS, Page JT, Roberts AW, Romanel E, Sanders WS, Szadkowski E, Tan X, Tang H, Xu C, Wang J, Wang Z, Zhang D, Zhang L, Ashrafi H, Bedon F, Bowers JE, Brubaker CL, Chee PW, Das S, Gingle AR, Haigler CH, Harker D, Hoffmann LV, Hovav R, Jones DC, Lemke C, Mansoor S, ur Rahman M, Rainville LN, Rambani A, Reddy UK, Rong JK, Saranga Y, Scheffler BE, Scheffler JA, Stelly DM, Triplett BA, Van Deynze A, Vaslin MF, Waghmare VN, Walford SA, Wright RJ, Zaki EA, Zhang T, Dennis ES, Mayer KF, Peterson DG, Rokhsar DS, Wang X, Schmutz J. Repeated polyploidization of Gossypium genomes and the evolution of spinnable cotton fibres. Nature, 2012, 492: 423–427[16]Finn R D, Bateman A, Clements J, Coggill P, Eberhardt R Y, Eddy S R, Heger A, Hetherington K, Holm L, Mistry J, Sonnhammer E L, Tate J, Punta M. Pfam: the protein families database. Nucl Acids Res, 2014, 42: D222–D230[17]Eddy S R. Accelerated Profile HMM Searches. PLoS Comput Biol, 2011, 7: e1002195[18]Guo A Y, Zhu Q H, Chen X, Luo J C. GSDS: a gene structure display server. Hereditas, 2007, 29: 1023–1026[19]Thompson J D, Gibson T J, Higgins D G. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools, Nucl Acids Res, 1997, 25: 4876–4882[20]Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol,2011, 28: 2731–2739[21]蒋建雄, 张天真. 利用CTAB/酸酚法提取棉花组织总RNA. 棉花学报, 2003, 15: 166–167Jiang J X, Zhang T Z. Extraction of total RNA in cotton tissues with CTAB-acidic phenolic method. Cotton Sci, 2003, 15: 166–167[22]Xu Y H, Wang J W, Wang S, Wang J Y, Chen X Y. Characterization of GaWRKY1, a cotton transcription factor that regulates the sesquiterpene synthase gene (+)-delta-cadinene synthase-A. Plant physiol, 2004, 135: 507–515[23]Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2–△△CT method. Methods, 2001,25:402–408[24]Shindou H, Hishikawa D, Harayama T, Yuki K, Shimizu T. Recent progress on acyl CoA: lysophospholipid acyltransferase research. J Lipid Res, 2009, 50(Suppl): S46–51[25]Zhao L,Lv Y D, Cai C P, Tong X C, Chen X D, Zhang W, Du H, Guo X H, Guo W Z. New world tetraploid cottons contain genome assembly: integration of a high-density molecular genetic linkage map with DNA sequence information.BMC Genomics, 2012, 13: 539[26]Wendel J F. New World tetraploid cottons contain old world cytoplasm. Proc Natl Acad Sci USA,1989, 86: 4132–4136[27]Wendel A A, Lewin T M, Coleman R A. Glycerol-3-phosphate acyltransferases: rate limiting enzymes of triacylglycerol biosynthesis. Biochim Biophys Acta, 2009, 1791: 501–506[28]Brown A P, Slabas A R, Denton H. Substrate selectivity of plant and microbial lysophosphatidic acid acyltransferases. Phytochemistry, 2002, 61: 493–501[29]戚维聪. 油菜发育种子中油脂积累与Kennedy途径酶活性的关系研究.南京农业大学硕士学位论文, 江苏南京, 2008Qi W C. Studies on correlations of developing seed lipid accumulation with Kennedy pathway enzyme activities in Brassica napus. MS Thesis of Nanjing Agriculture University, Nanjing, China, 2008 (in Chinese with English abstract)[30]Wanjie S W, Welti R, Moreau R A, Chapman K D. Identification and quantification of glycerolipids in cotton fibers: reconciliation with metabolic pathway predictions from DNA databases. Lipids, 2005, 40: 773–785 |
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